FastQCFastQC Report
Mon 6 Feb 2017
SRR2077115_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077115_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348841
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38000.023.7438661
GGTATCA17200.023.5418471
TATCAAC49500.017.871422
TCAACGC49750.017.4274884
ATCAACG50500.017.3868033
CGTGCCG3350.017.07151410
CAACGCA50900.016.9892435
AACGCAG53250.016.2351156
GTGCCGA4500.015.6427611
ACCGTAT1552.6746056E-715.6112228
ACCGTGC7350.014.96437558
GTACATG98850.014.8713681
TACATGG99950.014.509112
CGCACGA3500.014.4542935
GTGTTAG6100.014.4304981
GCACCGT5350.014.3911896
ACATGGG104300.013.5664083
ACGCAGA64100.013.315267
GTCTTAT4800.012.83713151
CTACACG4150.012.7260864
Produced by FastQC (version 0.11.5)