FastQCFastQC Report
Mon 6 Feb 2017
SRR2077115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348841
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.024.5519641
GTATCAA44400.022.5978181
TATCAAC61100.016.2419972
TGCGATA1958.913048E-1015.79830413
TCAACGC62900.015.7422184
ATCAACG63200.015.6674933
CAACGCA63800.015.4854285
ACCGTGC6050.014.9116628
TATACCG1351.9548323E-514.6691975
AACGCAG68100.014.5713936
CGTGCCG3500.014.46028310
GTATAGC6600.014.3353771
GTGCCGA4000.014.30310711
GCACCGT5100.014.2368976
CGTCCGA1251.6693318E-414.08305911
CGATCGT1251.6769712E-414.07549142
CGTATAC1100.001017218614.0026243
TAGACTG7500.013.7890445
GTACATG96650.013.7733031
TACATGG97900.013.2609112
Produced by FastQC (version 0.11.5)