FastQCFastQC Report
Mon 6 Feb 2017
SRR2077114_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077114_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3619159
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58450.018.3373761
TATACCG902.2133229E-417.1147375
GTACATG115050.015.1889111
GTGTACG4150.014.8491871
GTCCTAT6550.014.7844151
TACATGG116800.014.6029172
TATCAAC71700.014.5185342
TCAACGC72850.014.10754
CAACGCA73150.014.0480885
GTAATAC3300.014.0058713
ACATGGG118250.013.959343
ATCAACG74300.013.8631483
GTATTAT5000.013.6453441
AACGCAG75350.013.63346
CTTACAC7450.012.9987383
CATGGGT22400.012.9684674
TAGCCCT9200.012.9172084
CATGGGG83250.012.7945164
TAGGACA5850.012.7904484
GTAGGAC4900.012.57673
Produced by FastQC (version 0.11.5)