##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077114_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3619159 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.35190247236996 31.0 30.0 34.0 24.0 34.0 2 29.37863161027189 31.0 30.0 34.0 23.0 34.0 3 29.49088918171321 31.0 30.0 34.0 23.0 34.0 4 32.917231599938 37.0 35.0 37.0 26.0 37.0 5 32.87248474023938 37.0 35.0 37.0 26.0 37.0 6 32.93766728679232 37.0 35.0 37.0 26.0 37.0 7 32.80463168376963 37.0 35.0 37.0 25.0 37.0 8 32.72205614619308 37.0 35.0 37.0 25.0 37.0 9 34.11145628031264 39.0 35.0 39.0 25.0 39.0 10 34.053318464317265 38.0 35.0 39.0 25.0 39.0 11 34.07610994708992 38.0 35.0 39.0 25.0 39.0 12 33.983061534461456 38.0 35.0 39.0 24.0 39.0 13 33.919545673456184 38.0 34.0 39.0 23.0 39.0 14 34.88919138396517 39.0 34.0 41.0 22.0 41.0 15 34.920865593360226 39.0 34.0 41.0 23.0 41.0 16 34.891261478150035 39.0 34.0 41.0 23.0 41.0 17 34.73065980245687 39.0 34.0 41.0 21.0 41.0 18 34.705164100278544 39.0 34.0 41.0 21.0 41.0 19 34.8298704201722 39.0 34.0 41.0 22.0 41.0 20 34.804335206052016 39.0 34.0 41.0 22.0 41.0 21 34.74867697163899 39.0 34.0 41.0 21.0 41.0 22 34.6754699088932 39.0 34.0 41.0 20.0 41.0 23 34.630517200266695 39.0 34.0 41.0 20.0 41.0 24 34.63011572578049 39.0 34.0 41.0 20.0 41.0 25 34.53596899169116 39.0 34.0 41.0 19.0 41.0 26 34.4807401388002 39.0 34.0 41.0 19.0 41.0 27 34.42136253201365 39.0 34.0 41.0 18.0 41.0 28 34.30966918004984 39.0 34.0 41.0 18.0 41.0 29 34.292271768109664 39.0 34.0 41.0 18.0 41.0 30 34.13578154482851 39.0 34.0 41.0 16.0 41.0 31 34.13180686452294 39.0 34.0 41.0 16.0 41.0 32 33.996108488187446 39.0 33.0 41.0 16.0 41.0 33 33.957216856181226 39.0 33.0 41.0 16.0 41.0 34 33.87583330823542 39.0 33.0 41.0 15.0 41.0 35 33.80939798444887 38.0 33.0 41.0 15.0 41.0 36 33.72379411902047 38.0 33.0 41.0 13.0 41.0 37 33.62397396743277 38.0 33.0 40.0 9.0 41.0 38 33.534393487547796 38.0 33.0 40.0 9.0 41.0 39 33.42201738028089 38.0 33.0 40.0 9.0 41.0 40 33.35723105837572 38.0 33.0 40.0 8.0 41.0 41 33.24450735654333 38.0 33.0 40.0 8.0 41.0 42 33.09737289795778 38.0 33.0 40.0 2.0 41.0 43 33.00966467624108 38.0 32.0 40.0 2.0 41.0 44 32.854320299273944 38.0 32.0 40.0 2.0 41.0 45 32.71064355006232 38.0 32.0 40.0 2.0 41.0 46 32.571879820698676 38.0 32.0 40.0 2.0 41.0 47 32.40582522072117 38.0 32.0 40.0 2.0 41.0 48 32.2392210455523 38.0 32.0 40.0 2.0 41.0 49 32.05986086822933 38.0 31.0 40.0 2.0 41.0 50 31.849001384023193 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.3429332106821832 1101 2 1.414984308629549 1101 3 1.4254606507067855 1101 4 1.6291203131900147 1101 5 1.7170843287257682 1101 6 1.634980770101869 1101 7 1.7646222740529538 1101 8 1.7993151775475908 1101 9 2.068367376621758 1101 10 2.053456939133355 1101 11 2.061008841409496 1101 12 1.9931236197749982 1101 13 2.094138864675898 1101 14 2.4753944138127295 1101 15 2.3923660719317468 1101 16 2.5164315087631337 1101 17 2.5898062382243054 1101 18 2.486898715768362 1101 19 2.451258065731494 1101 20 2.41903098563526 1101 21 2.4074240568391687 1101 22 2.4406359621184492 1101 23 2.4686685409145213 1101 24 2.503040445235044 1101 25 2.5950185042917795 1101 26 2.6114851512233557 1101 27 2.5357839888605938 1101 28 2.656299732852503 1101 29 2.6406183025536976 1101 30 2.685863479335012 1101 31 2.650765183186735 1101 32 2.752714057506914 1101 33 2.6245307955486084 1101 34 2.7156331723982063 1101 35 2.7246249436146854 1101 36 2.820401554001016 1101 37 2.579185463073486 1101 38 2.791966317261398 1101 39 2.751838270806438 1101 40 2.7455138597575015 1101 41 2.7904036534998014 1101 42 2.7897156749462013 1101 43 2.7947211680330284 1101 44 2.6932879675901304 1101 45 2.72868870817274 1101 46 2.6611868683428668 1101 47 2.7506973297274726 1101 48 2.732793193890359 1101 49 2.6982422526772183 1101 50 2.884353887767734 1102 1 0.9059491788205563 1102 2 0.9107617792442184 1102 3 0.9607994626203897 1102 4 1.077476695022817 1102 5 1.0970296800785988 1102 6 1.0859293917327264 1102 7 1.1962103618587605 1102 8 1.1895539545746985 1102 9 1.31167274100045 1102 10 1.3466809947659968 1102 11 1.249293328265047 1102 12 1.292483524643913 1102 13 1.2815683826456734 1102 14 1.637885379915815 1102 15 1.426335581666308 1102 16 1.509736064903791 1102 17 1.7645212902322172 1102 18 1.7325119671101135 1102 19 1.5817373685189153 1102 20 1.4798478115640066 1102 21 1.4343946660713698 1102 22 1.417437179354316 1102 23 1.4182084771313157 1102 24 1.357220637642719 1102 25 1.5798926968987246 1102 26 1.6429419223917705 1102 27 1.4271278477063802 1102 28 1.5483579271664496 1102 29 1.4794578432041021 1102 30 1.6712755176143403 1102 31 1.7301610502395093 1102 32 1.7705548099265584 1102 33 1.7116897154251802 1102 34 1.636962221384188 1102 35 1.7167244903254044 1102 36 1.7128757704730262 1102 37 1.7556962730367403 1102 38 1.6428732925907354 1102 39 1.7988305525740884 1102 40 1.7207951486355881 1102 41 1.702437671209914 1102 42 1.6674248520922887 1102 43 1.5986536428314295 1102 44 1.5882578761534276 1102 45 1.547176374313409 1102 46 1.4960015305449232 1102 47 1.454737461831769 1102 48 1.4260806741656822 1102 49 1.3476066983743493 1102 50 1.3590291450739862 1103 1 0.45977212043770876 1103 2 0.5537239528697775 1103 3 0.4762600239698358 1103 4 0.48519568121108136 1103 5 0.5099872259857889 1103 6 0.5022278925980288 1103 7 0.4881041527739711 1103 8 0.5997097180277535 1103 9 0.688202055598012 1103 10 0.6055648199621402 1103 11 0.6125468348518481 1103 12 0.6178671240595293 1103 13 0.6009789713341149 1103 14 0.7458974694933573 1103 15 0.6854984749504851 1103 16 0.5466439570017343 1103 17 0.6998094153845926 1103 18 0.6367721396642381 1103 19 0.7163238960419775 1103 20 0.6597973718361203 1103 21 0.4529908578602644 1103 22 0.6291356757498718 1103 23 0.640825098436828 1103 24 0.506973589405618 1103 25 0.6627955378897568 1103 26 0.6050017295184844 1103 27 0.5870371380202499 1103 28 0.7465856282357919 1103 29 0.8122351272146133 1103 30 0.842485154566667 1103 31 0.675334162358638 1103 32 0.8691210616807012 1103 33 0.7783213253841623 1103 34 0.7663273693288559 1103 35 0.7234581954534818 1103 36 0.6685119521693181 1103 37 0.7949675992147434 1103 38 0.669434299451801 1103 39 0.8001710039211289 1103 40 0.8439339879758663 1103 41 0.7757110434261776 1103 42 0.8213141046841059 1103 43 0.767134549325533 1103 44 0.812484298467055 1103 45 0.7052013817551028 1103 46 0.7814726059947859 1103 47 0.7925186073700559 1103 48 0.7662692232962627 1103 49 0.7056939328674687 1103 50 0.6671082299199291 1104 1 0.4310770409921716 1104 2 0.518225053657261 1104 3 0.527407587596727 1104 4 0.4056487815989698 1104 5 0.4539808559698173 1104 6 0.4456523979954454 1104 7 0.4204792123112995 1104 8 0.5219270855210496 1104 9 0.5717693460909743 1104 10 0.37545697617279217 1104 11 0.46032008792032997 1104 12 0.5548120316782388 1104 13 0.5369704216595323 1104 14 0.6023951864965582 1104 15 0.6052445815470762 1104 16 0.6428460809010446 1104 17 0.6822597614464883 1104 18 0.5420933940242563 1104 19 0.6087124583515902 1104 20 0.6537484879013533 1104 21 0.643536196823554 1104 22 0.6313835362924962 1104 23 0.6306643801510106 1104 24 0.6297935166789728 1104 25 0.5733061916624607 1104 26 0.5547849741542592 1104 27 0.7173032128432197 1104 28 0.4959598651450321 1104 29 0.655216342344346 1104 30 0.6015887269590863 1104 31 0.6100022530791236 1104 32 0.6787780667996941 1104 33 0.6279310930987592 1104 34 0.6388181370615555 1104 35 0.664509936160492 1104 36 0.6844037990024887 1104 37 0.6659569050496685 1104 38 0.7541676996555182 1104 39 0.7222184240468223 1104 40 0.7701848260855684 1104 41 0.7507450902390147 1104 42 0.7012293584462626 1104 43 0.7057925646358925 1104 44 0.7547617308072958 1104 45 0.8193755273801528 1104 46 0.8262800019513179 1104 47 0.8017010213247673 1104 48 0.8542767622697909 1104 49 0.8819164517250968 1104 50 0.8398041895877135 1105 1 0.5767312907072082 1105 2 0.45930442597839516 1105 3 0.43743263889229667 1105 4 0.508677078425265 1105 5 0.5154181895897594 1105 6 0.4670623114260266 1105 7 0.43179735182305734 1105 8 0.39124158809843834 1105 9 0.5079754262029681 1105 10 0.43589063727621635 1105 11 0.414029082234741 1105 12 0.44416074673614503 1105 13 0.5610811360053063 1105 14 0.639108958080115 1105 15 0.5921537624971762 1105 16 0.5261249321142856 1105 17 0.5927301988948983 1105 18 0.4655474775755337 1105 19 0.38671353848917533 1105 20 0.5007658081219333 1105 21 0.6376502451679968 1105 22 0.6764022364180846 1105 23 0.634215710204451 1105 24 0.5794509897468245 1105 25 0.5357689735870252 1105 26 0.5813243834564616 1105 27 0.5332508276328198 1105 28 0.6859212024586938 1105 29 0.6025015400970162 1105 30 0.6687382750779705 1105 31 0.41341428876557984 1105 32 0.6574453800378919 1105 33 0.6651880712822731 1105 34 0.6592754054589278 1105 35 0.6740743448473197 1105 36 0.5969195398386873 1105 37 0.5931096738405159 1105 38 0.655210786714072 1105 39 0.6295593687918881 1105 40 0.572185243566544 1105 41 0.677925033068739 1105 42 0.6740212301672557 1105 43 0.6609967099075718 1105 44 0.6825578419220619 1105 45 0.7070054235509531 1105 46 0.7951585074327951 1105 47 0.6411228321101063 1105 48 0.681106513382403 1105 49 0.761374425916344 1105 50 0.8371619712697758 1106 1 0.32255842530821255 1106 2 0.2726080652515286 1106 3 0.24421672309150466 1106 4 0.24729886941224066 1106 5 0.2895419499555203 1106 6 0.3003994156236871 1106 7 0.36795685818698587 1106 8 0.3407610273529542 1106 9 0.3276222278965051 1106 10 0.28742982329026034 1106 11 0.2952331491418434 1106 12 0.3284953792220193 1106 13 0.4033490535215378 1106 14 0.498571119102607 1106 15 0.3743088054745556 1106 16 0.31738679200632447 1106 17 0.4996664644044486 1106 18 0.15965349692245923 1106 19 0.3608258066969867 1106 20 0.36283971167578954 1106 21 0.42641752867025673 1106 22 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1107 9 0.15998569197684986 1107 10 0.04485930976888852 1107 11 -0.0359718229603061 1107 12 -0.025837210109330044 1107 13 0.08203726335676009 1107 14 -0.06114130679719665 1107 15 -0.01799597400485453 1107 16 -0.055842203270998425 1107 17 0.028837653305394895 1107 18 0.05095694039830789 1107 19 -0.03325213306641217 1107 20 0.022952138642260422 1107 21 0.0015912402446005558 1107 22 -0.19867222665038753 1107 23 -0.21461326914072032 1107 24 -0.12417091114552647 1107 25 -0.05492251783819313 1107 26 0.13416148024595032 1107 27 0.10840658583711615 1107 28 0.04980185119904945 1107 29 0.05457060044420814 1107 30 0.03464225026355905 1107 31 -0.0010426243813839164 1107 32 0.09630893783373295 1107 33 0.10192054131816519 1107 34 0.1389830878061531 1107 35 0.23146672793275513 1107 36 0.029775188816117293 1107 37 0.008941621363341312 1107 38 0.06825300932136713 1107 39 0.13013998675561567 1107 40 0.1936895742782596 1107 41 0.1890697082373407 1107 42 0.1281567214855741 1107 43 0.004798408398315246 1107 44 0.1594456261856294 1107 45 0.2502957299465436 1107 46 0.20238679158817519 1107 47 0.17060589677292626 1107 48 0.12617539872523054 1107 49 0.21761772855062844 1107 50 0.2624251890571152 1108 1 0.017458767659878305 1108 2 -0.04082911947897472 1108 3 0.031162823377627547 1108 4 -0.08092842704918013 1108 5 0.015972549909683664 1108 6 -0.07643721009781501 1108 7 -0.10989938010300193 1108 8 -0.013050913135977282 1108 9 -0.0810596591250814 1108 10 -0.022883254826879806 1108 11 -0.07894009166911786 1108 12 -0.054697079590638964 1108 13 0.01429326130466535 1108 14 -0.05060938693521422 1108 15 -0.027183637508180425 1108 16 -0.03387511292434198 1108 17 -0.12405650954232783 1108 18 -0.054078785965394616 1108 19 1.0788415021067976E-4 1108 20 -0.19413295689519572 1108 21 -0.12357160302290993 1108 22 -0.18633095996621307 1108 23 -0.33169499692333915 1108 24 -0.2658711028141951 1108 25 -0.04656048231553456 1108 26 -0.12968046151465984 1108 27 -0.25309261892683566 1108 28 -0.27438752903783836 1108 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46 -2.331226705097137 2216 47 -2.2885282398108835 2216 48 -2.348852820508771 2216 49 -2.406409790646755 2216 50 -2.576803805790796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 164950.0 3 8515.0 4 16077.0 5 12600.0 6 10542.0 7 9199.0 8 8371.0 9 7732.0 10 7697.0 11 7633.0 12 7847.0 13 8442.0 14 8828.0 15 9399.0 16 9892.0 17 10602.0 18 11796.0 19 13139.0 20 15960.0 21 20339.0 22 24297.0 23 28059.0 24 30394.0 25 33243.0 26 37080.0 27 42506.0 28 48357.0 29 55907.0 30 65575.0 31 79279.0 32 96807.0 33 120655.0 34 154488.0 35 202912.0 36 269201.0 37 369725.0 38 574943.0 39 1016171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.99107530021858 16.307654357041088 12.22519601596446 25.476074326775873 2 16.805461135981332 19.01819807072461 37.61229629572266 26.5640444975714 3 20.521636092774397 22.600579632537976 27.416679897128777 29.46110437755885 4 13.859222918298023 14.930931940972748 34.48166939439008 36.72817574633915 5 15.603317067720102 36.240427945214115 32.4998070861209 15.656447900944878 6 35.17554152073687 33.654489477019304 16.458558886336768 14.711410115907059 7 31.052082593234488 30.23451584196271 19.652205837835208 19.061195726967593 8 27.846547416399343 33.516625854613245 18.87023647029973 19.766590258687685 9 27.209625160736834 15.768010335817294 17.84280456087568 39.179559942570194 10 15.948385841213868 27.98595809155437 31.761787355039743 24.303868712192017 11 37.136864651361314 21.83262821973006 21.64218672941551 19.388320399493114 12 24.89159656338159 23.592331196413426 28.21851213311201 23.297560107092973 13 29.32100582841734 20.42624880538593 24.54459528534862 25.708150080848107 14 23.336964998616356 19.941436287219847 25.192487014331576 31.529111699832217 15 25.173073276026507 27.548438677516152 22.053939258896392 25.224548787560952 16 25.381041994448072 26.5346943052223 23.61347602139934 24.470787678930286 17 24.313930937626566 26.554193684833287 24.91865867329801 24.213216704242136 18 24.33793829880761 26.15579841365068 25.43989538609553 24.06636790144618 19 25.212603066659955 25.856094031409793 25.266075247025437 23.665227654904815 20 25.46982442431698 25.18065916296915 24.995071360887813 24.35444505182606 21 25.682300033100486 24.99009335885878 25.12888311988543 24.19872348815531 22 25.438892196633216 24.708020507215934 24.946344023784786 24.906743272366057 23 24.915254471586298 24.912379164529767 25.212821104753797 24.959545259130138 24 24.81020106865336 25.158316711956637 25.025861875875716 25.00562034351429 25 24.92374990355528 24.977731293802844 25.145510543102013 24.953008259539867 26 24.911874239347547 25.205474678932422 25.23069494839662 24.651956133323417 27 24.90283036293993 25.114562836180955 25.16771025861392 24.814896542265195 28 24.893689932218773 25.225971988628178 25.08845689086993 24.791881188283117 29 24.861976237014815 25.388656775194107 24.994137239480494 24.755229748310583 30 24.8942530515627 25.471602630400035 25.045738873618017 24.588405444419248 31 25.08331893423644 24.934250267823202 24.790434391954935 25.191996405985417 32 24.73446576336186 25.40995389850151 24.87988066026104 24.975699677875593 33 24.938390040667826 25.154612383919762 24.83004387480613 25.076953700606285 34 25.03080333607663 25.120289895538196 24.983088228992706 24.86581853939247 35 25.146507958581594 25.11291694670696 24.98974990316665 24.7508251915448 36 25.027480791092906 25.289211826582385 24.838018153910124 24.845289228414586 37 25.13753767270139 24.884151518561332 25.16814307069736 24.81016773803991 38 25.120114425231048 25.14997025474763 24.9202738774761 24.80964144254522 39 25.17071253060385 25.220944865519996 24.86503342924567 24.743309174630486 40 25.206490931429197 25.079925730721893 25.05482280318973 24.65876053465918 41 24.897105747565547 25.290659504542994 24.98973752670662 24.822497221184843 42 25.20973328284071 25.19577409894159 24.8839176380508 24.710574980166903 43 24.966428890890164 24.906667064710284 24.97848076703005 25.14842327736951 44 24.96980143858483 25.023039360127726 24.874879827692673 25.132279373594763 45 24.800209395257873 25.268667714380005 24.735478179057267 25.195644711304855 46 24.986139355992744 24.93887773834089 24.932023421862727 25.14295948380364 47 25.009508078549995 25.093864635917985 24.882448086996707 25.014179198535313 48 24.836416510718227 25.26712993787213 24.94760491661146 24.948848634798185 49 25.053970489591315 24.900440304119666 24.922329756305725 25.123259449983294 50 24.987251726510078 25.117981989073385 25.056265144188846 24.83850114022769 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2272.0 1 1665.0 2 1058.0 3 1086.5 4 1115.0 5 1075.5 6 1036.0 7 1017.0 8 998.0 9 1133.0 10 1268.0 11 1469.0 12 1670.0 13 2311.0 14 2952.0 15 3675.0 16 4398.0 17 4698.0 18 4998.0 19 5029.0 20 5060.0 21 5451.0 22 5842.0 23 7013.5 24 8185.0 25 10362.0 26 12539.0 27 16323.5 28 20108.0 29 25845.5 30 31583.0 31 40799.0 32 50015.0 33 62207.0 34 74399.0 35 89789.5 36 105180.0 37 119609.0 38 134038.0 39 148993.0 40 163948.0 41 178616.5 42 193285.0 43 207448.5 44 221612.0 45 235651.5 46 249691.0 47 265808.5 48 281926.0 49 287457.5 50 292989.0 51 294117.0 52 295245.0 53 289170.0 54 283095.0 55 274312.5 56 265530.0 57 251289.5 58 237049.0 59 217157.5 60 197266.0 61 174464.5 62 151663.0 63 132037.5 64 112412.0 65 94801.5 66 77191.0 67 63505.5 68 49820.0 69 40820.0 70 31820.0 71 24946.5 72 18073.0 73 15416.5 74 12760.0 75 9699.0 76 6638.0 77 5067.5 78 3497.0 79 2763.5 80 2030.0 81 1620.5 82 1211.0 83 973.5 84 736.0 85 578.5 86 421.0 87 334.5 88 248.0 89 186.0 90 124.0 91 88.0 92 52.0 93 43.5 94 35.0 95 30.0 96 25.0 97 19.5 98 14.0 99 26.5 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0864012882550891 2 0.09673518074226636 3 0.10402969308615621 4 0.10532833732919719 5 0.0982548708139101 6 0.05866003676544744 7 0.05421148946481765 8 0.06001394246563912 9 0.061395478894406136 10 0.04702750003522918 11 0.04904454322122902 12 0.05843899093684472 13 0.05200103117879043 14 0.052553645750297244 15 0.052636537936023256 16 0.05631142483654352 17 0.05509567277922854 18 0.056615362850872264 19 0.06424144393766618 20 0.07023731203851502 21 0.08007385141133617 22 0.08471581381199335 23 0.059516589351283 24 0.07825022332536372 25 0.08559999712640423 26 0.08344480029752768 27 0.07744893219667885 28 0.0982548708139101 29 0.086069719512185 30 0.08231194042593873 31 0.056200901922242155 32 0.05313389105037938 33 0.056615362850872264 34 0.05128263223583158 35 0.05857714457972143 36 0.0576653305367352 37 0.05940606643698163 38 0.04904454322122902 39 0.054349643107694356 40 0.056670624308022936 41 0.04448547300629787 42 0.040589540277174894 43 0.04047901736287353 44 0.039705356962764 45 0.030974046732956468 46 0.027658359303915636 47 0.03268715190462757 48 0.026967591089532127 49 0.027022852546682807 50 0.02823860460399778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3619159.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.67059731406867 #Duplication Level Percentage of deduplicated Percentage of total 1 70.237352528646 33.48256548799367 2 13.323158350021236 12.70245833310868 3 5.839748575536189 8.351529082793755 4 3.211700670220468 6.124147573736176 5 1.9705525435380036 4.696870839460698 6 1.2628211036208818 3.6119661786251314 7 0.858390225711673 2.8643982351763575 8 0.5894773009046947 2.2480588029769435 9 0.4570239445412902 1.960794398080359 >10 2.1096915245057404 17.11381686820867 >50 0.10117302883946952 3.3102729038663012 >100 0.037798679337605584 3.0732495614483444 >500 8.190181112216168E-4 0.2674451151003392 >1k 2.92506465514795E-4 0.19242661942457884 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.526145715068058E-5 2 0.0 0.0 0.0 0.0 5.526145715068058E-5 3 0.0 0.0 0.0 2.763072857534029E-5 5.526145715068058E-5 4 0.0 0.0 0.0 2.763072857534029E-5 5.526145715068058E-5 5 0.0 0.0 0.0 2.763072857534029E-5 5.526145715068058E-5 6 0.0 0.0 0.0 2.763072857534029E-5 8.289218572602089E-5 7 0.0 0.0 0.0 2.763072857534029E-5 8.289218572602089E-5 8 0.0 0.0 0.0 2.763072857534029E-5 8.289218572602089E-5 9 0.0 0.0 0.0 2.763072857534029E-5 8.289218572602089E-5 10 0.0 0.0 0.0 2.763072857534029E-5 1.3815364287670146E-4 11 0.0 0.0 0.0 2.763072857534029E-5 1.3815364287670146E-4 12 0.0 0.0 0.0 2.763072857534029E-5 3.3156874290408354E-4 13 0.0 2.763072857534029E-5 0.0 2.763072857534029E-5 3.3156874290408354E-4 14 0.0 2.763072857534029E-5 0.0 2.763072857534029E-5 3.3156874290408354E-4 15 0.0 1.3815364287670146E-4 0.0 2.763072857534029E-5 4.144609286301044E-4 16 0.0 1.3815364287670146E-4 0.0 2.763072857534029E-5 4.144609286301044E-4 17 0.0 1.3815364287670146E-4 0.0 5.526145715068058E-5 4.144609286301044E-4 18 0.0 1.6578437145204177E-4 0.0 5.526145715068058E-5 4.4209165720544467E-4 19 0.0 1.9341510002738205E-4 0.0 5.526145715068058E-5 4.4209165720544467E-4 20 0.0 1.9341510002738205E-4 0.0 8.289218572602089E-5 4.973531143561252E-4 21 0.0 1.9341510002738205E-4 0.0 1.6578437145204177E-4 4.973531143561252E-4 22 0.0 1.9341510002738205E-4 0.0 2.763072857534029E-4 5.249838429314655E-4 23 0.0 1.9341510002738205E-4 0.0 3.3156874290408354E-4 5.249838429314655E-4 24 0.0 2.486765571780626E-4 0.0 5.802453000821462E-4 5.249838429314655E-4 25 0.0 2.486765571780626E-4 0.0 6.631374858081671E-4 5.249838429314655E-4 26 0.0 2.486765571780626E-4 0.0 0.001132859871588952 5.249838429314655E-4 27 0.0 2.486765571780626E-4 0.0 0.0012433827858903132 5.249838429314655E-4 28 0.0 2.486765571780626E-4 0.0 0.002431504114629946 5.249838429314655E-4 29 0.0 3.0393801432874323E-4 0.0 0.003702517629095599 5.249838429314655E-4 30 0.0 3.0393801432874323E-4 0.0 0.005857714457972142 5.249838429314655E-4 31 0.0 3.0393801432874323E-4 0.0 0.01414693303057423 6.078760286574865E-4 32 0.0 3.0393801432874323E-4 0.0 0.023320334917587206 6.078760286574865E-4 33 0.0 3.0393801432874323E-4 0.0 0.03661071536232589 6.078760286574865E-4 34 0.0 3.0393801432874323E-4 0.0 0.05161420097873567 6.355067572328268E-4 35 0.0 3.0393801432874323E-4 0.0 0.0703478349528164 6.355067572328268E-4 36 0.0 3.0393801432874323E-4 0.0 0.09430367662763642 6.355067572328268E-4 37 0.0 3.868302000547641E-4 0.0 0.12491852388911347 6.355067572328268E-4 38 0.0 3.868302000547641E-4 0.0 0.1621094845515215 6.631374858081671E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5845 0.0 18.337376 1 TATACCG 90 2.2133229E-4 17.114737 5 GTACATG 11505 0.0 15.188911 1 GTGTACG 415 0.0 14.849187 1 GTCCTAT 655 0.0 14.784415 1 TACATGG 11680 0.0 14.602917 2 TATCAAC 7170 0.0 14.518534 2 TCAACGC 7285 0.0 14.1075 4 CAACGCA 7315 0.0 14.048088 5 GTAATAC 330 0.0 14.005871 3 ACATGGG 11825 0.0 13.95934 3 ATCAACG 7430 0.0 13.863148 3 GTATTAT 500 0.0 13.645344 1 AACGCAG 7535 0.0 13.6334 6 CTTACAC 745 0.0 12.998738 3 CATGGGT 2240 0.0 12.968467 4 TAGCCCT 920 0.0 12.917208 4 CATGGGG 8325 0.0 12.794516 4 TAGGACA 585 0.0 12.790448 4 GTAGGAC 490 0.0 12.5767 3 >>END_MODULE