FastQCFastQC Report
Mon 6 Feb 2017
SRR2077114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3619159
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC1801.4551915E-1118.33802214
GTATCAA67550.018.1743351
GGTATCA38750.016.6926521
AACGTCG1105.6238867E-516.00077422
GCACCGT6150.015.3830716
TCTATAC3650.015.0700563
TGCGATA2203.0377123E-1015.00425113
ACCGTGC8150.014.5796088
GTACATG109800.014.3870521
CGTGCCG4450.014.34094310
TACATGG109950.014.1280812
AGCACCG7300.013.8646435
TATCAAC88350.013.6971382
CTATACG1150.001423711713.39269354
TCAACGC89950.013.3799314
ATCAACG90950.013.3295843
ACCGTCG2155.4616976E-813.3049928
CAACGCA90500.013.29879955
ACATGGG113350.013.18001653
CTAGTAC2704.6929927E-1013.0383883
Produced by FastQC (version 0.11.5)