FastQCFastQC Report
Mon 6 Feb 2017
SRR2077113_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077113_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3282658
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC43870.13364170132861847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43900.020.9031521
CCGTATA600.007420773618.330759
TATCATA9400.017.0894722
TATAGGC12450.016.4364285
ATAGGCC12700.016.2814186
TCAACGC56550.015.9934744
ATCAACG57400.015.9099863
GGTATCA23850.015.8701231
CTTATAG14900.015.6550043
GTACTAG2252.5465852E-1115.6486791
CAACGCA58400.015.4477385
TATCAAC59500.015.2744892
AACGCAG60250.014.9690716
TCTTATA16350.014.9395952
GTACATG97250.014.8441191
TACATGG97500.014.6704132
TACCGCG1655.424372E-714.66683517
TAGGCCT14000.014.6122267
GTGTATA6000.014.3038711
TTATAGG14750.014.1731114
Produced by FastQC (version 0.11.5)