##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077113_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3282658 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.61014854425895 31.0 30.0 34.0 25.0 34.0 2 29.720041807583975 31.0 30.0 34.0 25.0 34.0 3 29.799912144365937 31.0 30.0 34.0 25.0 34.0 4 33.226592901240394 37.0 35.0 37.0 27.0 37.0 5 33.200831155728075 37.0 35.0 37.0 27.0 37.0 6 33.323033956019785 37.0 35.0 37.0 27.0 37.0 7 33.20864616417549 37.0 35.0 37.0 26.0 37.0 8 33.14460690087118 37.0 35.0 37.0 26.0 37.0 9 34.586329127189 39.0 35.0 39.0 27.0 39.0 10 34.51889200763527 39.0 35.0 39.0 27.0 39.0 11 34.56726287051529 39.0 35.0 39.0 27.0 39.0 12 34.47804005168982 39.0 35.0 39.0 27.0 39.0 13 34.412776780279884 39.0 35.0 39.0 26.0 39.0 14 35.42750051939617 40.0 36.0 41.0 25.0 41.0 15 35.46551727289288 40.0 36.0 41.0 26.0 41.0 16 35.44644857916968 40.0 36.0 41.0 26.0 41.0 17 35.29313379584471 40.0 35.0 41.0 25.0 41.0 18 35.26726268773658 40.0 35.0 41.0 25.0 41.0 19 35.372743977593764 40.0 35.0 41.0 25.0 41.0 20 35.35440457093002 40.0 35.0 41.0 25.0 41.0 21 35.30777071507297 40.0 35.0 41.0 25.0 41.0 22 35.240973625641175 40.0 35.0 41.0 25.0 41.0 23 35.21178447465438 39.0 35.0 41.0 25.0 41.0 24 35.2004107646913 39.0 35.0 41.0 25.0 41.0 25 35.11102161723822 39.0 35.0 41.0 24.0 41.0 26 35.069158285755016 39.0 35.0 41.0 24.0 41.0 27 35.01206126255004 39.0 35.0 41.0 24.0 41.0 28 34.91219645787042 39.0 35.0 41.0 23.0 41.0 29 34.90249121291344 39.0 35.0 41.0 24.0 41.0 30 34.75281037500708 39.0 34.0 41.0 22.0 41.0 31 34.754810888006 39.0 34.0 41.0 23.0 41.0 32 34.63363256239304 39.0 34.0 41.0 22.0 41.0 33 34.596352102473055 39.0 34.0 41.0 22.0 41.0 34 34.51574273043369 39.0 34.0 41.0 21.0 41.0 35 34.45373109230386 39.0 34.0 41.0 20.0 41.0 36 34.3747923176889 39.0 34.0 41.0 19.0 41.0 37 34.29293822262325 39.0 34.0 41.0 18.0 41.0 38 34.212527165486016 39.0 34.0 41.0 18.0 41.0 39 34.1161567851418 39.0 34.0 41.0 16.0 41.0 40 34.05182111569344 39.0 33.0 41.0 16.0 41.0 41 33.9460196584597 39.0 33.0 41.0 15.0 41.0 42 33.8069805017763 38.0 33.0 40.0 15.0 41.0 43 33.73540892776524 38.0 33.0 40.0 14.0 41.0 44 33.60832745902863 38.0 33.0 40.0 9.0 41.0 45 33.47981909781647 38.0 33.0 40.0 8.0 41.0 46 33.353561047175795 38.0 33.0 40.0 6.0 41.0 47 33.218288045845775 38.0 33.0 40.0 2.0 41.0 48 33.05342042942031 38.0 33.0 40.0 2.0 41.0 49 32.8943703547552 38.0 33.0 40.0 2.0 41.0 50 32.69500386576975 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6235532300646263 1101 2 0.7606155354825752 1101 3 0.7980840321460967 1101 4 0.8552877775460033 1101 5 1.067140894072402 1101 6 1.1838228058951188 1101 7 1.2414344877082542 1101 8 1.2793238432279992 1101 9 1.4190121873336494 1101 10 1.4110892870939793 1101 11 1.4039820593611694 1101 12 1.4100757573904303 1101 13 1.3594604256264446 1101 14 1.7215988118502352 1101 15 1.7976590556651644 1101 16 1.7294837965693404 1101 17 1.829417529013952 1101 18 1.7489495478605974 1101 19 1.6601925455337536 1101 20 1.8014284513144787 1101 21 1.7041750193843086 1101 22 1.7309978389633116 1101 23 1.7252429984763324 1101 24 1.7723417872342395 1101 25 1.687760121600462 1101 26 1.868341214191858 1101 27 1.6136292605923117 1101 28 1.802899698805433 1101 29 1.7335570154024396 1101 30 1.8143273787175929 1101 31 1.9501278066705083 1101 32 1.8597774107345018 1101 33 1.7788440573087954 1101 34 1.864105475841093 1101 35 1.8275355786335155 1101 36 1.8693082251529347 1101 37 1.9053131068056857 1101 38 1.9923625117456467 1101 39 1.9467953748641378 1101 40 1.9120214842904915 1101 41 1.9127109044519415 1101 42 2.0293157911589006 1101 43 1.8521631767800812 1101 44 1.790780662286437 1101 45 1.7822095567298248 1101 46 1.6445070931188894 1101 47 1.7425024155659372 1101 48 1.660072188684211 1101 49 1.8037579715088512 1101 50 1.8595765594360856 1102 1 0.31670794627323673 1102 2 0.4154818711872359 1102 3 0.45609280093330895 1102 4 0.6015678669398667 1102 5 0.43142258709661263 1102 6 0.6131061196095686 1102 7 0.7206427378745914 1102 8 0.7575426193509642 1102 9 0.7949244653417367 1102 10 0.802859592636743 1102 11 0.7542993734509409 1102 12 0.7872265839424557 1102 13 0.8327181836147375 1102 14 1.0553441417033511 1102 15 0.8472137923456486 1102 16 0.9997014808588816 1102 17 1.2647554002799666 1102 18 1.0982253552202366 1102 19 1.1284926973668448 1102 20 1.0555219736714747 1102 21 1.013038489026222 1102 22 0.9960540513249114 1102 23 0.967370708867513 1102 24 0.8395663125426012 1102 25 1.090749868602181 1102 26 1.0540885229930623 1102 27 0.8374244053455442 1102 28 1.0903410554084658 1102 29 0.9489621026638133 1102 30 1.103312656026226 1102 31 1.0486410366208645 1102 32 1.1793715557777489 1102 33 1.178473004542532 1102 34 1.1851590950983066 1102 35 1.1820555984229983 1102 36 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-2.1221198702044966 2216 50 -2.2211510018832143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 128083.0 3 6537.0 4 12306.0 5 9673.0 6 8373.0 7 7340.0 8 6629.0 9 6178.0 10 6184.0 11 6306.0 12 6220.0 13 6572.0 14 6999.0 15 7261.0 16 7801.0 17 8725.0 18 9616.0 19 10707.0 20 12874.0 21 16548.0 22 20317.0 23 23176.0 24 25516.0 25 27984.0 26 31449.0 27 35856.0 28 41135.0 29 47776.0 30 55948.0 31 67358.0 32 82966.0 33 103609.0 34 133755.0 35 175727.0 36 235682.0 37 329321.0 38 530529.0 39 1023622.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.42480790608023 16.161804637084114 12.761890127339015 25.651497329496635 2 16.306698296154458 18.913447817443377 37.92461977534119 26.855234111060973 3 19.923022668427553 22.868890625652746 27.218091563505233 29.989995142414465 4 13.307804148584001 15.450279686672863 34.96379198044511 36.27812418429803 5 15.295926304353724 36.40375149672938 33.23780487618957 15.062517322727325 6 35.056491699066946 33.882370225149934 16.74155611557542 14.319581960207703 7 30.92973823738459 30.288163199621575 20.082243519330152 18.69985504366368 8 28.282801497072512 33.226316849868766 18.918813473408797 19.57206817964992 9 27.168232281143677 15.86849691865856 17.49189353293216 39.4713772672656 10 15.430730718100913 28.595182671906155 32.17361861590046 23.80046799409247 11 36.34680448177462 21.961494140247005 22.358189040236674 19.3335123377417 12 24.95053850620345 23.468659655240206 28.45108028901179 23.129721549544552 13 28.738153117677605 20.522110116573078 24.91511817013671 25.8246185956126 14 22.998415117342276 19.79481865284974 25.481834806461446 31.724931423346543 15 24.471137493279496 27.572383331443667 22.294486687611474 25.66199248766536 16 24.769416599078856 26.589578462362805 23.639214280641664 25.001790657916672 17 24.2424907420107 26.713512854508604 25.06280193236715 23.981194471113547 18 24.10165956955248 26.438236209394283 25.880129025139663 23.579975195913576 19 24.98005744553023 25.600113147515767 26.073217294134466 23.346612112819535 20 25.430455824093823 25.385492837015118 24.996494411176915 24.187556927714148 21 25.608040666730485 25.259273576685775 24.909347994558743 24.223337762024997 22 25.086508355106506 25.012027329018334 25.066478050505637 24.834986265369523 23 25.018814037451286 25.478092962224096 25.077244997020536 24.425848003304075 24 24.522570569135084 25.45698874741138 25.466805153186233 24.5536355302673 25 24.882247506305692 25.169749872941026 24.944626068568247 25.003376552185035 26 24.593873368062237 25.330076495248733 25.387971548194198 24.68807858849483 27 24.7125573018143 25.474274735924645 25.219596618396515 24.59357134386454 28 23.755310079363046 25.338790076387085 25.82412187783189 25.081777966417977 29 24.30525766039006 25.772106158708784 25.151732172098594 24.770904008802557 30 24.652772908390826 25.53709761503355 25.16223442363565 24.647895052939976 31 24.723695315507474 25.382602093517946 25.583157491765192 24.310545099209392 32 24.84425518011538 25.428709713600213 24.93486757728113 24.79216752900328 33 24.462181485345607 25.739486170381053 24.88807163171734 24.910260712556003 34 24.478537741743374 25.56992217763639 25.120555478683425 24.830984601936812 35 24.851524893068966 25.42280925113073 25.418603027932065 24.30706282786824 36 24.921659665997844 25.57800856295436 24.94586051229595 24.554471258751853 37 24.8529542203518 25.123682440248224 25.32881652265116 24.694546816748815 38 25.025083051415685 25.255188808631253 24.994026393587514 24.725701746365548 39 24.940565958375448 25.35059988759346 24.902436734748616 24.806397419282476 40 24.61078968591906 25.096544396401693 25.692208844350624 24.600457073328634 41 24.594855203553717 25.361046367045216 25.370158620252486 24.673939809148578 42 24.50327069267374 25.472578140832535 25.41056221172878 24.613588954764943 43 24.586561897929716 24.633340911431937 26.152424615695928 24.627672574942416 44 25.019945329379823 25.134558597214053 25.221623235927797 24.623872837478324 45 24.553261133543405 25.427099816651417 25.36411468717538 24.655524362629798 46 24.272027189799317 25.590015780882492 25.10659484877137 25.03136218054682 47 24.846030808160375 25.265208523849036 25.152399865555967 24.73636080243462 48 24.620586664261516 25.37622914929203 25.614327071709404 24.388857114737057 49 25.14552847312111 24.81863788274354 25.135473113797413 24.900360530337935 50 24.729819125761818 25.34835484247435 24.940009744702802 24.98181628706103 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1647.0 1 1187.0 2 727.0 3 697.0 4 667.0 5 707.5 6 748.0 7 763.0 8 778.0 9 837.0 10 896.0 11 1081.5 12 1267.0 13 1752.0 14 2237.0 15 2872.0 16 3507.0 17 3667.0 18 3827.0 19 3950.5 20 4074.0 21 4289.5 22 4505.0 23 5323.0 24 6141.0 25 7678.0 26 9215.0 27 12103.5 28 14992.0 29 20521.5 30 26051.0 31 33568.5 32 41086.0 33 51968.5 34 62851.0 35 80090.5 36 97330.0 37 108471.0 38 119612.0 39 137351.5 40 155091.0 41 177929.5 42 200768.0 43 207498.5 44 214229.0 45 226962.5 46 239696.0 47 257291.5 48 274887.0 49 282762.0 50 290637.0 51 284407.5 52 278178.0 53 268708.0 54 259238.0 55 258167.0 56 257096.0 57 234071.0 58 211046.0 59 188161.0 60 165276.0 61 142909.0 62 120542.0 63 101098.0 64 81654.0 65 66578.0 66 51502.0 67 41901.5 68 32301.0 69 25978.0 70 19655.0 71 15573.5 72 11492.0 73 9550.5 74 7609.0 75 5819.5 76 4030.0 77 3222.5 78 2415.0 79 1889.5 80 1364.0 81 1049.5 82 735.0 83 601.0 84 467.0 85 353.0 86 239.0 87 189.0 88 139.0 89 106.5 90 74.0 91 57.5 92 41.0 93 36.0 94 31.0 95 23.5 96 16.0 97 14.0 98 12.0 99 26.0 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08383450240628174 2 0.09193769195572613 3 0.09903559859114171 4 0.09973625031910117 5 0.09160259765104986 6 0.05550380210183333 7 0.050873408073579394 8 0.056509085015862145 9 0.059220302571879255 10 0.04325762842184595 11 0.04712644448492655 12 0.05553426522044027 13 0.04953303085487431 14 0.05050785065029619 15 0.05038599817586846 16 0.05303628949467169 17 0.052031006580642886 18 0.053219068206313294 19 0.06046929043476354 20 0.06647052480032949 21 0.07713261631275631 22 0.08005707569902196 23 0.056752789964717616 24 0.07417769380788374 25 0.08179347345961717 26 0.0787471615989238 27 0.07429954628231147 28 0.09279065927672027 29 0.08228088335732812 30 0.07701076383832857 31 0.053889256815665844 32 0.05026414570144072 33 0.053767404341238105 34 0.0486191372966663 35 0.05498592908551546 36 0.05425481423894905 37 0.057179273625214695 38 0.04746153878960281 39 0.05209193281785674 40 0.05434620359476985 41 0.041490767542643796 42 0.03786565642841867 43 0.03874908686801976 44 0.03619018490503732 45 0.02836116342305534 46 0.025558556511217436 47 0.030310803013899105 48 0.024827441664651024 49 0.025162535969327295 50 0.02665522878106705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3282658.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.245135458484064 #Duplication Level Percentage of deduplicated Percentage of total 1 75.26046709348441 35.55690962500453 2 12.67779637065721 11.979284136935549 3 4.840300639593399 6.860419781321314 4 2.3703315342062474 4.47946537660362 5 1.303081186112883 3.0782123575652616 6 0.8008506623227737 2.270177881407367 7 0.5131582074811524 1.697096031685594 8 0.3628332638801842 1.3713685360690557 9 0.27467982919612427 1.167955716427574 >10 1.36515110003877 11.812041084504612 >50 0.11924831167895761 3.9354067676054294 >100 0.0968845069979398 9.169239917555954 >500 0.01116805722729348 3.638222523529026 >1k 0.004049237122773548 2.9842002637850733 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC 4387 0.13364170132861847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5231559303466885E-4 2 0.0 0.0 0.0 0.0 1.5231559303466885E-4 3 0.0 0.0 0.0 0.0 1.5231559303466885E-4 4 0.0 0.0 0.0 0.0 1.5231559303466885E-4 5 0.0 0.0 0.0 0.0 1.8277871164160264E-4 6 0.0 0.0 0.0 0.0 2.4370494885547016E-4 7 0.0 0.0 0.0 0.0 2.4370494885547016E-4 8 0.0 0.0 0.0 0.0 2.4370494885547016E-4 9 3.046311860693377E-5 0.0 0.0 0.0 2.4370494885547016E-4 10 3.046311860693377E-5 0.0 0.0 0.0 3.046311860693377E-4 11 3.046311860693377E-5 0.0 0.0 0.0 3.046311860693377E-4 12 3.046311860693377E-5 0.0 0.0 0.0 7.311148465664106E-4 13 3.046311860693377E-5 0.0 0.0 0.0 7.92041083780278E-4 14 3.046311860693377E-5 0.0 0.0 0.0 7.92041083780278E-4 15 3.046311860693377E-5 0.0 0.0 0.0 0.0010052829140288144 16 3.046311860693377E-5 0.0 0.0 0.0 0.0010357460326357482 17 3.046311860693377E-5 0.0 0.0 0.0 0.0010357460326357482 18 3.046311860693377E-5 0.0 0.0 0.0 0.0010357460326357482 19 3.046311860693377E-5 0.0 0.0 0.0 0.0010966722698496157 20 3.046311860693377E-5 0.0 0.0 0.0 0.0012185247442773508 21 3.046311860693377E-5 0.0 0.0 0.0 0.0013099141000981521 22 3.046311860693377E-5 0.0 0.0 3.046311860693377E-5 0.001462229693132821 23 3.046311860693377E-5 0.0 0.0 9.138935582080132E-5 0.001462229693132821 24 3.046311860693377E-5 0.0 0.0 2.741680674624039E-4 0.001462229693132821 25 3.046311860693377E-5 0.0 0.0 4.874098977109403E-4 0.001462229693132821 26 3.046311860693377E-5 0.0 0.0 6.70188609352543E-4 0.001584082167560556 27 3.046311860693377E-5 0.0 0.0 0.0010052829140288144 0.0016145452861674899 28 3.046311860693377E-5 0.0 0.0 0.001584082167560556 0.0016145452861674899 29 3.046311860693377E-5 0.0 0.0 0.0028330700304448407 0.0016145452861674899 30 3.046311860693377E-5 0.0 0.0 0.004539004672433132 0.0016145452861674899 31 3.046311860693377E-5 0.0 0.0 0.010052829140288145 0.0016145452861674899 32 3.046311860693377E-5 0.0 0.0 0.016175915980281834 0.0016450084047744237 33 3.046311860693377E-5 0.0 0.0 0.02522346220654116 0.0016450084047744237 34 3.046311860693377E-5 0.0 0.0 0.03667759480274826 0.0016754715233813573 35 3.046311860693377E-5 0.0 0.0 0.05062970312472393 0.0016754715233813573 36 3.046311860693377E-5 0.0 0.0 0.06802414384928311 0.0016754715233813573 37 3.046311860693377E-5 0.0 0.0 0.08934832687413675 0.0016754715233813573 38 3.046311860693377E-5 0.0 0.0 0.11469364155510565 0.0017668608792021586 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4390 0.0 20.903152 1 CCGTATA 60 0.0074207736 18.33075 9 TATCATA 940 0.0 17.089472 2 TATAGGC 1245 0.0 16.436428 5 ATAGGCC 1270 0.0 16.281418 6 TCAACGC 5655 0.0 15.993474 4 ATCAACG 5740 0.0 15.909986 3 GGTATCA 2385 0.0 15.870123 1 CTTATAG 1490 0.0 15.655004 3 GTACTAG 225 2.5465852E-11 15.648679 1 CAACGCA 5840 0.0 15.447738 5 TATCAAC 5950 0.0 15.274489 2 AACGCAG 6025 0.0 14.969071 6 TCTTATA 1635 0.0 14.939595 2 GTACATG 9725 0.0 14.844119 1 TACATGG 9750 0.0 14.670413 2 TACCGCG 165 5.424372E-7 14.666835 17 TAGGCCT 1400 0.0 14.612226 7 GTGTATA 600 0.0 14.303871 1 TTATAGG 1475 0.0 14.173111 4 >>END_MODULE