FastQCFastQC Report
Mon 6 Feb 2017
SRR2077113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3282658
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC43190.13157020926334698No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC32920.10028458645402598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45300.021.953921
CGCGTAA654.926822E-420.3124710
TACACCG2000.019.8025445
GGTATCA23350.018.657361
AATATCG10450.017.47087133
TATCATA9400.017.0870442
ATATCGA10750.016.77843934
TATCGAA11150.016.37379635
GCGTTAT953.3580637E-416.2044643
ACTTTAT10350.015.9438341
TCAACGC63850.015.6444464
TATAGGC13800.015.62519555
ATCAACG63850.015.5755283
ATAGGCC13850.015.5687876
GCGTAAC850.00297672815.53306411
CTTATAG16350.015.4755233
CAACGCA64500.015.4190355
TTATAGG14250.015.2857084
TCGCACG1301.3671222E-515.23458413
TCGAAAA11950.015.09357337
Produced by FastQC (version 0.11.5)