FastQCFastQC Report
Mon 6 Feb 2017
SRR2077112_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077112_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2875460
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43800.017.5339451
TATACCG1408.364077E-817.2893185
GGTATCA25600.016.1601811
CGATCGA1004.9368513E-415.40401625
GTACATG81300.014.50783351
GTCCTAG7450.014.1779451
TACATGG82250.014.1262322
TATCAAC55600.013.5752442
CAACGCA55000.013.5628275
TCAACGC55200.013.4747614
GTACTAA2805.820766E-1113.3603931
ATCAACG56400.013.1880653
ACATGGG86500.013.1527893
AACGCAG56950.013.1322666
ATCGTTG2552.7848728E-912.94387522
TACGCTC1701.1888702E-512.93891934
CGTGCCG2901.07320375E-1012.89250810
CTAGCAC5650.012.8531313
CGCAACG1200.001960991412.83489217
TGGACCG3850.012.574055
Produced by FastQC (version 0.11.5)