FastQCFastQC Report
Mon 6 Feb 2017
SRR2077112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2875460
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA755.4681215E-520.53717410
GTATCAA56450.018.3563061
GGTATCA33250.017.5340631
CGAATCG1857.601557E-915.45616637
GTACCGA2850.014.6686456
TACCGAT2254.274625E-1014.6686447
TCGCGTA1402.7552893E-514.145019
TATCAAC72150.014.0884832
ATCAACG74650.013.6758493
TCAACGC74550.013.5763764
CAACGCA75550.013.3095375
AACGCAG77500.013.0882176
GCGTAAC1200.00195976912.83595811
GTCTAGG5000.012.7601661
TCTATAC3900.012.4114983
TTATCGG1609.689579E-512.37107344
GCACCGT4450.012.36121756
GTATAGC4700.012.1703711
ACCGTGC5800.012.1397788
CGACTCA2201.0397434E-612.00099221
Produced by FastQC (version 0.11.5)