FastQCFastQC Report
Mon 6 Feb 2017
SRR2077111_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077111_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783016
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.023.781951
GTATCAA27350.021.4058321
TATACTC1801.4551915E-1118.3391725
GCACCGT4850.016.7803976
GTATACG953.3374142E-416.2174381
TATCAAC36000.016.201062
CGATACT2403.6379788E-1215.58325115
ACCGTGC5650.015.5734588
ATCAACG38250.015.1907873
CAACGCA38400.015.1298175
TCAACGC38250.015.0751654
GCGATAC2355.0931703E-1114.97783714
GTCTACG1351.9499153E-514.6729221
GTACATG77600.014.5500171
AGCACCG6550.014.4473485
AACGCAG40400.014.3735776
GTATTAG2451.0004442E-1014.3734731
TACATGG78250.014.2320082
ATAGGAC3450.014.0349683
CTATACT3300.014.0054664
Produced by FastQC (version 0.11.5)