##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077111_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2783016 Sequences flagged as poor quality 0 Sequence length 50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.62851668836974 31.0 30.0 34.0 16.0 34.0 2 28.654563250804163 31.0 30.0 34.0 16.0 34.0 3 28.75847210364583 31.0 30.0 34.0 16.0 34.0 4 32.135507665065525 35.0 33.0 37.0 19.0 37.0 5 32.04219522992322 35.0 33.0 37.0 19.0 37.0 6 32.181049264538906 35.0 33.0 37.0 19.0 37.0 7 31.951915116549817 35.0 33.0 37.0 17.0 37.0 8 31.90247127576701 35.0 33.0 37.0 17.0 37.0 9 33.25995718314232 38.0 33.0 39.0 17.0 39.0 10 33.157258887480346 38.0 33.0 39.0 17.0 39.0 11 33.20051196256148 38.0 33.0 39.0 17.0 39.0 12 33.09586793608086 38.0 33.0 39.0 17.0 39.0 13 33.03279355921777 38.0 33.0 39.0 16.0 39.0 14 33.92155345136356 39.0 33.0 41.0 16.0 41.0 15 33.962717785309174 39.0 33.0 41.0 16.0 41.0 16 33.92003315827146 39.0 33.0 41.0 16.0 41.0 17 33.7436949697738 39.0 33.0 41.0 11.0 41.0 18 33.7048342517614 38.0 33.0 41.0 11.0 41.0 19 33.82114691399546 39.0 33.0 41.0 11.0 41.0 20 33.809957973651606 39.0 33.0 41.0 11.0 41.0 21 33.73818619799527 39.0 33.0 41.0 10.0 41.0 22 33.65767893537083 39.0 33.0 41.0 10.0 41.0 23 33.60448520597797 39.0 33.0 41.0 10.0 41.0 24 33.611091707701284 39.0 33.0 41.0 10.0 41.0 25 33.51159030346933 39.0 33.0 41.0 10.0 41.0 26 33.448972266059556 39.0 33.0 41.0 9.0 41.0 27 33.39577458412025 38.0 33.0 41.0 8.0 41.0 28 33.268523788580445 38.0 32.0 40.0 8.0 41.0 29 33.24943191127899 38.0 32.0 40.0 8.0 41.0 30 33.08749284948416 38.0 32.0 40.0 7.0 41.0 31 33.081050198777156 38.0 32.0 40.0 2.0 41.0 32 32.95223347619992 38.0 32.0 40.0 2.0 41.0 33 32.911333244221375 38.0 32.0 40.0 2.0 41.0 34 32.82289070562297 38.0 32.0 40.0 2.0 41.0 35 32.74812541501738 38.0 32.0 40.0 2.0 41.0 36 32.655895618278876 38.0 32.0 40.0 2.0 41.0 37 32.5481635750567 38.0 31.0 40.0 2.0 41.0 38 32.44863845554607 38.0 31.0 40.0 2.0 41.0 39 32.34074148334037 38.0 31.0 40.0 2.0 41.0 40 32.25237296515723 38.0 31.0 40.0 2.0 41.0 41 32.14178826136824 38.0 31.0 40.0 2.0 41.0 42 31.99074277690103 38.0 31.0 40.0 2.0 41.0 43 31.872846940154133 38.0 31.0 40.0 2.0 41.0 44 31.717687932803837 38.0 30.0 40.0 2.0 41.0 45 31.558873897958186 38.0 30.0 40.0 2.0 41.0 46 31.404516898214023 38.0 30.0 40.0 2.0 41.0 47 31.239428734868934 37.0 30.0 40.0 2.0 41.0 48 31.05935287472296 37.0 30.0 40.0 2.0 41.0 49 30.868212040462577 37.0 30.0 40.0 2.0 41.0 50 30.64286946248243 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25067137169133247 1101 2 0.4375656382308435 1101 3 0.4456711785009091 1101 4 0.6175444083317956 1101 5 0.5877558547205908 1101 6 0.48152904733021984 1101 7 0.46720846198738997 1101 8 0.3424390087267213 1101 9 0.9523073765505217 1101 10 0.8074227788229251 1101 11 0.8993520810713491 1101 12 0.8041557784240823 1101 13 0.9043541456231168 1101 14 1.0831505322366368 1101 15 1.2156058528692455 1101 16 1.1416072291001882 1101 17 1.281926312586279 1101 18 1.2358382413149513 1101 19 1.1509735303921218 1101 20 1.1890936919906494 1101 21 1.1555781216355072 1101 22 1.1572362891147705 1101 23 1.345733731009858 1101 24 1.1981483561866852 1101 25 1.2756894879685774 1101 26 1.4108631030484133 1101 27 1.3678222690201807 1101 28 1.329744520623727 1101 29 1.2860385670753445 1101 30 1.4670269184883793 1101 31 1.443618815569451 1101 32 1.4479191759063141 1101 33 1.3074627475323766 1101 34 1.3353986370279287 1101 35 1.4397666295515066 1101 36 1.5120236328887344 1101 37 1.5838579806063748 1101 38 1.6829746470861622 1101 39 1.6351907580280596 1101 40 1.4902863046954664 1101 41 1.6295962192504376 1101 42 1.6429881334714267 1101 43 1.552141131435583 1101 44 1.346653950050957 1101 45 1.5306654669583608 1101 46 1.5595278789633298 1101 47 1.4538609716659359 1101 48 1.2576224145653399 1101 49 1.2830195114255254 1101 50 1.4427233202857934 1102 1 0.45095201806990204 1102 2 0.5069899031072076 1102 3 0.46555451713462404 1102 4 0.6746509955663953 1102 5 0.6470474096870902 1102 6 0.7347090417709552 1102 7 0.6309342183975915 1102 8 0.8057564749200026 1102 9 0.8361514836473845 1102 10 0.8739693412354725 1102 11 0.8048047499613418 1102 12 0.8973790527237924 1102 13 0.7984350806978497 1102 14 1.0999001248817777 1102 15 0.9756726622778089 1102 16 0.9317900479561061 1102 17 1.160544486310016 1102 18 1.242114803468617 1102 19 1.1612997374011371 1102 20 1.0751388285903403 1102 21 1.0607004849304644 1102 22 1.1040024782933315 1102 23 1.064125346841017 1102 24 1.0618045178882767 1102 25 1.13348404809242 1102 26 1.247177028660495 1102 27 1.059232377240619 1102 28 1.1843352298529481 1102 29 1.1166696491719748 1102 30 1.202435508210094 1102 31 1.1264753694050142 1102 32 1.1231828582203534 1102 33 1.206208020883878 1102 34 1.1629428357146807 1102 35 1.2305235149964986 1102 36 1.2594135478728319 1102 37 1.130820236767434 1102 38 1.115739412624933 1102 39 1.3331672961268666 1102 40 1.124927689756774 1102 41 1.1966955900258398 1102 42 1.1996507544614374 1102 43 1.0713991002942649 1102 44 0.8681571655230158 1102 45 0.8728890849799669 1102 46 0.8464362696135623 1102 47 0.7600134424670522 1102 48 0.754195116092852 1102 49 0.8233469334602752 1102 50 0.870554665644292 1103 1 0.227931677984607 1103 2 0.37976045399636504 1103 3 0.3375709750399558 1103 4 0.35688775803938455 1103 5 0.3746326656390053 1103 6 0.31364642742476434 1103 7 0.40511555103002905 1103 8 0.49351837068427784 1103 9 0.5183289406017906 1103 10 0.4684345929630993 1103 11 0.4711005436895235 1103 12 0.3659251592179871 1103 13 0.4416907789697575 1103 14 0.5091863576803064 1103 15 0.4629741554436535 1103 16 0.37513321365841534 1103 17 0.6188472227901158 1103 18 0.43071443073047533 1103 19 0.6126586054709193 1103 20 0.44547361710903033 1103 21 0.42262091715276284 1103 22 0.40511794088392605 1103 23 0.5487202622367349 1103 24 0.42164820614248555 1103 25 0.47553076100687974 1103 26 0.4434146550369107 1103 27 0.427848750594535 1103 28 0.5258234332103271 1103 29 0.5931588202666873 1103 30 0.6632672999611628 1103 31 0.5546120878684491 1103 32 0.7226701643108484 1103 33 0.657507320730673 1103 34 0.6613249378214476 1103 35 0.5394355844839183 1103 36 0.5426277414763874 1103 37 0.6398138891066623 1103 38 0.7026386462439476 1103 39 0.749747516626492 1103 40 0.6588395113169057 1103 41 0.7123535932924341 1103 42 0.7164730636344387 1103 43 0.7196803337623621 1103 44 0.6825241277124334 1103 45 0.6578816231898301 1103 46 0.598781743765123 1103 47 0.5710420228013042 1103 48 0.5041659394852864 1103 49 0.47906677340236214 1103 50 0.5883599621459403 1104 1 0.3826626880071622 1104 2 0.48001886194857235 1104 3 0.4046794925316526 1104 4 0.44498781663717324 1104 5 0.4725495359076106 1104 6 0.3915502328785081 1104 7 0.45645647503926057 1104 8 0.478187194245578 1104 9 0.5282454595210453 1104 10 0.4127314768346224 1104 11 0.47251068906032145 1104 12 0.4279897560624093 1104 13 0.4625355288489814 1104 14 0.5904548871052171 1104 15 0.596211131481077 1104 16 0.59800463206129 1104 17 0.6641123352634537 1104 18 0.6316399672207567 1104 19 0.6700994765007806 1104 20 0.6593385430665037 1104 21 0.6859802047049115 1104 22 0.6851595963566126 1104 23 0.6262141601135696 1104 24 0.6088651829633207 1104 25 0.6148130824765019 1104 26 0.6270789676365425 1104 27 0.660543706950854 1104 28 0.6230585749509032 1104 29 0.4937063792393701 1104 30 0.6579349404880475 1104 31 0.6727307545293115 1104 32 0.5809968471970919 1104 33 0.8193717513172203 1104 34 0.5903804609106018 1104 35 0.6328232925584985 1104 36 0.7008880715627726 1104 37 0.7833527604918302 1104 38 0.7454423349373442 1104 39 0.7309230255192318 1104 40 0.7340199636317166 1104 41 0.7508140817234263 1104 42 0.6618964020151239 1104 43 0.7545284127558247 1104 44 0.788856368882108 1104 45 0.8211732126855935 1104 46 0.782254555141563 1104 47 0.843441038548324 1104 48 0.74760672584312 1104 49 0.826506967847326 1104 50 0.7966507781192007 1105 1 0.3496767345624221 1105 2 0.4030579728183632 1105 3 0.33333129925143723 1105 4 0.3842987642157283 1105 5 0.2877306750456299 1105 6 0.28893283099604616 1105 7 0.36322871443960736 1105 8 0.3393261614170697 1105 9 0.36779647375125535 1105 10 0.3643226892152569 1105 11 0.3965469054091457 1105 12 0.34689559884104426 1105 13 0.387449311205458 1105 14 0.5062995263685721 1105 15 0.4930486210667553 1105 16 0.3536208916717598 1105 17 0.43795796383739116 1105 18 0.3324755956425349 1105 19 0.34760632839757477 1105 20 0.4241978359256606 1105 21 0.44180228149367196 1105 22 0.31751555169401513 1105 23 0.2576541088808355 1105 24 0.5452425817278268 1105 25 0.40278465674834507 1105 26 0.41883474833633727 1105 27 0.5769154090375608 1105 28 0.6201712082015121 1105 29 0.5645961154223187 1105 30 0.4835705244896147 1105 31 0.3335994631906942 1105 32 0.5342194343377074 1105 33 0.4526030302961317 1105 34 0.4363040661655333 1105 35 0.42651332761683136 1105 36 0.47469424517049674 1105 37 0.5183906658228707 1105 38 0.43969790007634657 1105 39 0.5707018534718173 1105 40 0.605611773976662 1105 41 0.5624820737886509 1105 42 0.6563634169426038 1105 43 0.5490113378050054 1105 44 0.7408584681639354 1105 45 0.6520991257090145 1105 46 0.8062767033123137 1105 47 0.5523314063310742 1105 48 0.5332095606210459 1105 49 0.6481137673421529 1105 50 0.6257594135513074 1106 1 0.5143799017234407 1106 2 0.42229887500165475 1106 3 0.34906643512147184 1106 4 0.27099888791267546 1106 5 0.44335798665322557 1106 6 0.4450599163099298 1106 7 0.4150884992074353 1106 8 0.5207460728476931 1106 9 0.5999585540188335 1106 10 0.6601269391165019 1106 11 0.5342823388920266 1106 12 0.5953911247535331 1106 13 0.6483327793255498 1106 14 0.6549142773259788 1106 15 0.7128011192285157 1106 16 0.48226803051483813 1106 17 0.7605073288914213 1106 18 0.45445887946367947 1106 19 0.5924708747140244 1106 20 0.4715240363167723 1106 21 0.6345734246636994 1106 22 0.6040506261910323 1106 23 0.4981467789435854 1106 24 0.5849844347045092 1106 25 0.7031536915446281 1106 26 0.6505534933970054 1106 27 0.7331830765880696 1106 28 0.7473326986405056 1106 29 0.6365487769884481 1106 30 0.6697574844719938 1106 31 0.5227961166652477 1106 32 0.7066769326266922 1106 33 0.6459459602304065 1106 34 0.8426886585580036 1106 35 0.6525731621924038 1106 36 0.6482718266471537 1106 37 0.6805297363506853 1106 38 0.6716520625570297 1106 39 0.6619921028472291 1106 40 0.7980028567965647 1106 41 0.738384530790924 1106 42 0.6404811194844697 1106 43 0.6991535777297955 1106 44 0.7154134431431274 1106 45 0.715995834002932 1106 46 0.7176559229960873 1106 47 0.6669887878879699 1106 48 0.8228966070859158 1106 49 0.7542855331347162 1106 50 0.7804776952303065 1107 1 0.3263654769638684 1107 2 0.3040357957481383 1107 3 0.27821943902765156 1107 4 0.10531304225197147 1107 5 0.14985417858402883 1107 6 0.2171519310603074 1107 7 0.21857197864677147 1107 8 0.29791921493630014 1107 9 0.12682719378450713 1107 10 0.18012665771661318 1107 11 0.17829061908391708 1107 12 0.06343110665348917 1107 13 0.2225526797753119 1107 14 0.08304034749355083 1107 15 0.21467014215029678 1107 16 0.1501008317427477 1107 17 0.1742164282333718 1107 18 0.3106438355740906 1107 19 0.2421682065154016 1107 20 0.3328722784844942 1107 21 0.33809250621464315 1107 22 -0.0035402867028864193 1107 23 -0.07134052803682067 1107 24 -0.09530237208758052 1107 25 0.07502400337842374 1107 26 0.24300594623677085 1107 27 0.2507663740973527 1107 28 0.1589897395045483 1107 29 0.13265030808025102 1107 30 0.09869182558544765 1107 31 0.1524147620212304 1107 32 0.20967663037305329 1107 33 0.2346784572198004 1107 34 0.25989239283509846 1107 35 0.2301524246282014 1107 36 0.10482114537047238 1107 37 0.13880398617499878 1107 38 0.10951135306247295 1107 39 0.21607909712199813 1107 40 0.17396638134028564 1107 41 0.3428601961069546 1107 42 0.16878675795947373 1107 43 0.2607168646916094 1107 44 0.2702979548678286 1107 45 0.399746924741585 1107 46 0.2699548721936118 1107 47 0.36678876783822645 1107 48 0.21168834880302256 1107 49 0.327213908570414 1107 50 0.347464311901593 1108 1 0.08340293374632779 1108 2 0.02398014949758931 1108 3 0.10828955150443775 1108 4 -0.03016918777551325 1108 5 0.1220627340951097 1108 6 -0.033415899550426786 1108 7 -0.040014038933563256 1108 8 -0.028842504081119102 1108 9 -0.03603787854912355 1108 10 -0.06354640457111316 1108 11 -0.04968061636179044 1108 12 -0.16306037626211634 1108 13 -0.26199474221325403 1108 14 -0.2004914404770446 1108 15 -0.12080749066539909 1108 16 -0.13908302824616925 1108 17 -0.20914131529517732 1108 18 -0.1454227100659793 1108 19 -0.1808037609863078 1108 20 -0.21670115522795896 1108 21 -0.1505303939963767 1108 22 -0.22125993973332214 1108 23 -0.3335137699655917 1108 24 -0.10371521969550912 1108 25 0.03445176044376552 1108 26 -0.2078382667480554 1108 27 -0.04799062977978963 1108 28 -0.14935914689585417 1108 29 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2216 10 -1.0425843438363387 2216 11 -1.1216937856006766 2216 12 -1.1258714149977749 2216 13 -1.0480861538312993 2216 14 -1.111418026294153 2216 15 -1.2588614295101763 2216 16 -1.3218125046127938 2216 17 -1.2631978911718562 2216 18 -1.1986899493499408 2216 19 -1.198535430507377 2216 20 -1.432442287199592 2216 21 -1.5234124666933226 2216 22 -1.2838794064960197 2216 23 -1.3292947631637801 2216 24 -1.5227786391729055 2216 25 -1.52422192793545 2216 26 -1.4246822627740556 2216 27 -1.5355052082187832 2216 28 -1.6628848355685122 2216 29 -1.7943140830702404 2216 30 -1.6752832477885775 2216 31 -1.7028857270178968 2216 32 -1.6114515488182555 2216 33 -1.677976471569533 2216 34 -1.8049956965889429 2216 35 -1.6988278710199332 2216 36 -1.6920249234182592 2216 37 -1.866992204618132 2216 38 -1.851866473758701 2216 39 -1.886678776639485 2216 40 -1.9457048957435212 2216 41 -1.834281430014542 2216 42 -1.858173206573099 2216 43 -2.105505952185567 2216 44 -2.2053688356684695 2216 45 -2.31898432030259 2216 46 -2.366698205134412 2216 47 -2.3381579436273263 2216 48 -2.4732051479050483 2216 49 -2.44793437745448 2216 50 -2.5351685677904214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 169222.0 3 8437.0 4 15207.0 5 12017.0 6 10149.0 7 8591.0 8 7502.0 9 6931.0 10 6824.0 11 6916.0 12 7193.0 13 7513.0 14 7763.0 15 8298.0 16 8825.0 17 9618.0 18 10282.0 19 11620.0 20 13412.0 21 16897.0 22 20023.0 23 22717.0 24 25220.0 25 27538.0 26 31356.0 27 35474.0 28 40810.0 29 46303.0 30 54402.0 31 65633.0 32 78329.0 33 97625.0 34 123802.0 35 159275.0 36 210933.0 37 287625.0 38 438485.0 39 664249.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.378421027458124 15.488902642385552 12.031256799080234 26.101419531076093 2 17.049541165617363 18.17743034762796 37.417256057099394 27.355772429655286 3 20.9652113861493 21.58199710814252 26.601887620404142 30.850903885304042 4 14.111887835205806 14.38021433748352 33.24209273090556 38.26580509640511 5 16.319468047188945 35.388471502450905 31.942237025863573 16.349823424496574 6 36.645824285496516 32.49782851916512 15.91993850826457 14.936408687073794 7 32.09843966317748 29.39996254012467 18.75118410234527 19.75041369435258 8 28.619774111843938 33.33218286047101 18.003246490608365 20.044796537076685 9 27.578356837927952 15.254497487274588 17.023903428509385 40.143242246288075 10 16.209941021623248 27.686514128820683 31.2905325811766 24.813012268379474 11 38.01413345757087 21.229699960600595 20.80399947084013 19.95216711098841 12 25.386570047822875 22.77439552704031 27.730437452409014 24.108596972727803 13 30.051943299156232 19.660443464703253 23.866056820914793 26.421556415225723 14 23.84158766085765 19.150346628550228 24.23825841622173 32.769807294370395 15 25.61918672683669 26.69760248598595 21.493827635383454 26.189383151793905 16 26.137201532794048 25.73074291762221 22.854075748844092 25.277979800739647 17 25.09109838625716 25.888478894222335 24.09588266930495 24.924540050215562 18 25.17437868269587 25.42775894362577 24.564727368159936 24.833135005518425 19 26.002947460915944 25.122843828794156 24.372711789879617 24.501496920410286 20 25.975198502230867 24.597880845087538 24.561637535987124 24.865283116694467 21 26.214598046689584 24.38225479337773 24.31062382233218 25.092523337600507 22 26.119532067259126 24.048241764050765 24.299569876155548 25.53265629253456 23 25.716724297980136 24.174700176956534 24.46540934770557 25.643166177357763 24 25.438587028438935 24.39014216591585 24.37370908630639 25.797561719338823 25 25.823411353437294 24.06372458940462 24.447070407249193 25.665793649908892 26 25.406188170971937 24.619199713469317 24.576802665655944 25.397809449902798 27 25.616056704904793 24.485633554045783 24.380095749936892 25.518213991112532 28 25.789254102812176 24.444524368708212 24.26250085419057 25.503720674289042 29 25.665198237885463 24.569846264496988 24.319446192573945 25.4455093050436 30 25.66157258473415 24.643007917654668 24.404198104624662 25.291221392986525 31 25.64138975839964 24.272418128277593 24.282016999709157 25.80417511361361 32 25.50633325496303 24.65317547110257 24.254781127568876 25.585710146365525 33 25.70409522056497 24.43266139005689 24.18532030670294 25.677923082675196 34 25.86664317991451 24.39100409460368 24.1833620327065 25.558990692775307 35 25.621579412281775 24.438981370863164 24.412161338671932 25.527277878183124 36 25.805862471746593 24.631254172624136 24.09582807692045 25.467055278708823 37 25.793259456250965 24.130899270061445 24.386411918602665 25.689429355084926 38 25.81084316473106 24.42695814912569 24.24415849969839 25.518040186444857 39 25.963144140785516 24.44520100532321 24.143647183466452 25.448007670424825 40 25.90241636623271 24.23711105966065 24.433945034606825 25.426527539499816 41 25.58763921270904 24.45758177701954 24.359626930429414 25.59515207984201 42 25.88957328663325 24.30143982332186 24.24425091516631 25.564735974878577 43 25.667875032126226 24.1761038391514 24.44824667190272 25.707774456819653 44 25.83655696610207 24.29848694743244 24.185620860528427 25.679335225937056 45 25.67530023027868 24.404472445450335 24.114818021616077 25.80540930265491 46 25.788790613906553 24.279120118433152 24.164683615776568 25.767405651883728 47 25.62450960976169 24.304486017116528 24.103423773004536 25.967580600117245 48 25.57900404116787 24.50966156836417 24.28564343126924 25.625690959198728 49 25.853751806915408 24.211372623155977 24.145313736582462 25.789561833346152 50 25.810775015122346 24.33724962773698 24.294587189695456 25.557388167445218 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1273.0 1 923.5 2 574.0 3 616.5 4 659.0 5 616.5 6 574.0 7 607.0 8 640.0 9 706.0 10 772.0 11 944.5 12 1117.0 13 1518.0 14 1919.0 15 2488.5 16 3058.0 17 3217.5 18 3377.0 19 3466.0 20 3555.0 21 3573.5 22 3592.0 23 4001.0 24 4410.0 25 5141.0 26 5872.0 27 6910.5 28 7949.0 29 9835.0 30 11721.0 31 15243.5 32 18766.0 33 24826.5 34 30887.0 35 40805.0 36 50723.0 37 62555.0 38 74387.0 39 89689.5 40 104992.0 41 121011.5 42 137031.0 43 151421.5 44 165812.0 45 180470.0 46 195128.0 47 206598.0 48 218068.0 49 225714.5 50 233361.0 51 239189.0 52 245017.0 53 243009.5 54 241002.0 55 235538.0 56 230074.0 57 218302.5 58 206531.0 59 189256.0 60 171981.0 61 152332.0 62 132683.0 63 114509.0 64 96335.0 65 81614.0 66 66893.0 67 56201.0 68 45509.0 69 36584.5 70 27660.0 71 22113.0 72 16566.0 73 13430.0 74 10294.0 75 7978.5 76 5663.0 77 4255.0 78 2847.0 79 2239.5 80 1632.0 81 1270.5 82 909.0 83 715.0 84 521.0 85 397.0 86 273.0 87 218.5 88 164.0 89 133.0 90 102.0 91 68.0 92 34.0 93 31.0 94 28.0 95 27.0 96 26.0 97 20.5 98 15.0 99 27.5 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08296754312587495 2 0.0934597573280211 3 0.10046654420959131 4 0.10136485022004904 5 0.09410653765555066 6 0.054042089589136394 7 0.049334966094337934 8 0.05573090488879691 9 0.056557346418418 10 0.04128614424063678 11 0.044843436042049344 12 0.05397022510829977 13 0.04775394751593235 14 0.04901157593057316 15 0.04825699888178868 16 0.051347171557763235 17 0.05059259450897875 18 0.05123937483650831 19 0.05889294204560808 20 0.0651451518783938 21 0.07531397591677517 22 0.07865567427567789 23 0.05540751472503212 24 0.07330177045334989 25 0.08038042181575672 26 0.07768550378438356 27 0.07265499012582033 28 0.09363941853011265 29 0.08142245678788768 30 0.07696685897601739 31 0.05170649396194632 32 0.04850852456471684 33 0.05145496827901816 34 0.04692750598631125 35 0.054365479752901166 36 0.053646834944534996 37 0.055659040407960284 38 0.04606513221627184 39 0.05113157811525337 40 0.05332344478077021 41 0.04031597374934244 42 0.03621969834165524 43 0.036686817467093256 44 0.0344230863207398 45 0.026410196707456944 46 0.024685449167378123 47 0.028494266651718857 48 0.023427820752737315 49 0.0233559562719007 50 0.02547595845658092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2783016.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.20069874432463 #Duplication Level Percentage of deduplicated Percentage of total 1 79.99741443061387 48.95897660892887 2 11.012330660771001 13.47924662485471 3 3.6915802742340484 6.7778187676166795 4 1.7331854771833923 4.242886490285573 5 0.9393059669811096 2.8743090756978713 6 0.5938818918844153 2.1807592052956606 7 0.3760533694098925 1.611031028113014 8 0.27062895774747076 1.3250145051674813 9 0.19875499098816635 1.0947549894657955 >10 1.0967631680619092 11.948746535652974 >50 0.0642410268790791 2.676189766991538 >100 0.024915667481019303 2.438621819154764 >500 9.441177646262868E-4 0.391644582775028 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.155934425098526E-4 2 0.0 0.0 0.0 0.0 2.155934425098526E-4 3 0.0 0.0 0.0 0.0 2.155934425098526E-4 4 0.0 0.0 0.0 0.0 2.155934425098526E-4 5 0.0 0.0 0.0 0.0 2.155934425098526E-4 6 3.593224041830877E-5 0.0 0.0 0.0 2.8745792334647016E-4 7 3.593224041830877E-5 0.0 0.0 0.0 2.8745792334647016E-4 8 3.593224041830877E-5 0.0 0.0 3.593224041830877E-5 2.8745792334647016E-4 9 3.593224041830877E-5 0.0 0.0 3.593224041830877E-5 2.8745792334647016E-4 10 3.593224041830877E-5 0.0 0.0 3.593224041830877E-5 2.8745792334647016E-4 11 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 2.8745792334647016E-4 12 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 4.6711912543801403E-4 13 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 4.6711912543801403E-4 14 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 4.6711912543801403E-4 15 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 5.389836062746316E-4 16 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 5.749158466929403E-4 17 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 5.749158466929403E-4 18 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 5.749158466929403E-4 19 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 6.108480871112491E-4 20 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 6.827125679478666E-4 21 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 7.545770487844841E-4 22 3.593224041830877E-5 0.0 0.0 7.186448083661754E-5 7.905092892027929E-4 23 3.593224041830877E-5 0.0 0.0 1.077967212549263E-4 7.905092892027929E-4 24 3.593224041830877E-5 0.0 0.0 1.4372896167323508E-4 7.905092892027929E-4 25 3.593224041830877E-5 0.0 0.0 2.8745792334647016E-4 7.905092892027929E-4 26 3.593224041830877E-5 0.0 0.0 5.030513658563228E-4 8.623737700394104E-4 27 3.593224041830877E-5 0.0 0.0 7.186448083661754E-4 8.983060104577193E-4 28 3.593224041830877E-5 0.0 0.0 0.0017247475400788209 8.983060104577193E-4 29 3.593224041830877E-5 0.0 0.0 0.0030542404355562453 8.983060104577193E-4 30 3.593224041830877E-5 0.0 0.0 0.00495864917772661 8.983060104577193E-4 31 3.593224041830877E-5 0.0 0.0 0.01052814644256447 9.342382508760281E-4 32 3.593224041830877E-5 0.0 0.0 0.01753493332413468 9.701704912943368E-4 33 3.593224041830877E-5 0.0 0.0 0.02518850053323445 0.0010061027317126456 34 3.593224041830877E-5 0.0 0.0 0.036758681947929875 0.0010061027317126456 35 3.593224041830877E-5 0.0 0.0 0.05199395188529279 0.0010061027317126456 36 3.593224041830877E-5 0.0 0.0 0.07006786881570211 0.0010061027317126456 37 3.593224041830877E-5 0.0 0.0 0.09651399776357736 0.0010061027317126456 38 3.593224041830877E-5 0.0 0.0 0.12385843272191033 0.0010420349721309544 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1490 0.0 23.78195 1 GTATCAA 2735 0.0 21.405832 1 TATACTC 180 1.4551915E-11 18.339172 5 GCACCGT 485 0.0 16.780397 6 GTATACG 95 3.3374142E-4 16.217438 1 TATCAAC 3600 0.0 16.20106 2 CGATACT 240 3.6379788E-12 15.583251 15 ACCGTGC 565 0.0 15.573458 8 ATCAACG 3825 0.0 15.190787 3 CAACGCA 3840 0.0 15.129817 5 TCAACGC 3825 0.0 15.075165 4 GCGATAC 235 5.0931703E-11 14.977837 14 GTCTACG 135 1.9499153E-5 14.672922 1 GTACATG 7760 0.0 14.550017 1 AGCACCG 655 0.0 14.447348 5 AACGCAG 4040 0.0 14.373577 6 GTATTAG 245 1.0004442E-10 14.373473 1 TACATGG 7825 0.0 14.232008 2 ATAGGAC 345 0.0 14.034968 3 CTATACT 330 0.0 14.005466 4 >>END_MODULE