FastQCFastQC Report
Mon 6 Feb 2017
SRR2077111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783016
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.026.0663661
GTATCAA30450.022.3274121
CTAGTAC1601.1023076E-917.8774763
TATCAAC41650.015.9539032
GTATTAC2252.5465852E-1115.64604951
TGCGATA1857.548806E-915.46410313
ATCAACG43750.015.0878043
GTACATG76900.015.0496571
TCAACGC44350.014.7847274
TACATGG76900.014.7065842
GCGATAC2407.094059E-1114.67080914
CCCCTAT2102.5447662E-914.668171
CAACGCA44750.014.5050645
GTATTAG2950.014.1709441
AACGCAG46650.014.0537686
TACCGAT3150.013.96968657
ACATGGG79950.013.62293
CGGGTTA1302.333035E-413.53790230
CGAATCG2959.094947E-1213.42076237
GTACGGG2003.2747266E-713.2013531
Produced by FastQC (version 0.11.5)