FastQCFastQC Report
Mon 6 Feb 2017
SRR2077110_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077110_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829556
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28600.023.1624471
GGTATCA14700.022.6070371
TATCAAC37450.017.6888122
GTATATG4300.017.4018121
TCAACGC37650.017.3604154
CGTTATT1154.2928514E-617.21069544
CAACGCA38150.017.1304635
ATCAACG39400.016.6457753
AACGCAG39550.016.4634866
TATACTG3900.016.3621565
CGTTACG1105.6144636E-516.0036824
CGCGTTG1958.949428E-1015.79235837
TCTATAC2900.015.1780513
GCGCGTT1756.434129E-815.0827836
GTACATG83050.014.9459481
GCGATAC2651.8189894E-1214.94286714
TATACGA900.004350176514.6721142
GCACCGT5600.014.5341726
TAGTACT3200.014.4423524
TACATGG84750.014.2566222
Produced by FastQC (version 0.11.5)