FastQCFastQC Report
Mon 6 Feb 2017
SRR2077110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829556
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.022.7742981
GTATCAA35950.022.274631
CGTACAA3000.020.53554525
CGTGCCG3300.020.00498410
AGCGTAC3250.018.27889323
TGCGATA2050.018.24844713
CGCGAAT1002.4833766E-517.60127328
GCGTACA3600.017.11295324
ATAATAC2201.8189894E-1217.0009293
GCACCGT5750.016.8368936
CGCGTAA800.001985928516.5041110
GCGATAC2151.2732926E-1116.37559314
TATCAAC49100.016.0413252
TCAACGC49350.015.8717434
CGTGCGA1401.5929145E-615.70959139
ATCAACG50500.015.6840263
GTGCCGA4700.015.45065811
CAACGCA51050.015.3006445
GTATTAG3350.015.1039691
ACGGTTG3950.015.03348236
Produced by FastQC (version 0.11.5)