FastQCFastQC Report
Mon 6 Feb 2017
SRR2077109_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077109_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154681
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34050.021.7159041
GGTATCA19300.019.4982031
TATCAAC41950.016.9963262
TCAACGC42800.016.7610824
ACCGTAT2109.094947E-1216.7595968
ATCAACG42900.016.619953
CAACGCA44700.015.9486675
AACGCAG46200.015.4733246
TATACTG6400.014.7834355
GTACATG83900.014.6624191
TACATGG83900.014.1898352
GCGATAC1901.7565253E-713.89414614
ACATGGG87750.013.1409313
GTATAGC6100.012.9875871
TACGTCA1701.1838574E-512.9443224
ATTATAC4150.012.7263883
TGTACTG10800.012.6314965
GTATTAC3153.092282E-1112.5752821
TATACAG9100.012.5732875
GTAATAC2654.9330993E-912.4562533
Produced by FastQC (version 0.11.5)