##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077109_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3154681 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.787745575543138 31.0 30.0 34.0 18.0 34.0 2 28.78166033269291 31.0 30.0 34.0 16.0 34.0 3 28.909948422677285 31.0 30.0 34.0 18.0 34.0 4 32.2813564984859 35.0 33.0 37.0 20.0 37.0 5 32.24186565931706 35.0 33.0 37.0 20.0 37.0 6 32.26585889349826 35.0 33.0 37.0 22.0 37.0 7 32.120295839737835 35.0 33.0 37.0 18.0 37.0 8 32.075743316043685 35.0 33.0 37.0 18.0 37.0 9 33.42943517902444 38.0 34.0 39.0 17.0 39.0 10 33.35176076440059 38.0 33.0 39.0 17.0 39.0 11 33.38841169677695 38.0 34.0 39.0 17.0 39.0 12 33.28025179091008 38.0 33.0 39.0 17.0 39.0 13 33.2275288690045 38.0 33.0 39.0 17.0 39.0 14 34.13724113468208 39.0 33.0 41.0 17.0 41.0 15 34.17030977141587 39.0 33.0 41.0 17.0 41.0 16 34.12783828222251 39.0 33.0 41.0 17.0 41.0 17 33.965057322753076 39.0 33.0 41.0 16.0 41.0 18 33.932363050336946 39.0 33.0 41.0 16.0 41.0 19 34.04585186267645 39.0 34.0 41.0 16.0 41.0 20 34.03190338420905 39.0 34.0 41.0 16.0 41.0 21 33.971697296810675 39.0 34.0 41.0 15.0 41.0 22 33.89352869592837 39.0 33.0 41.0 11.0 41.0 23 33.84092559596358 39.0 33.0 41.0 11.0 41.0 24 33.849653578285725 39.0 33.0 41.0 10.0 41.0 25 33.75242853397855 39.0 33.0 41.0 10.0 41.0 26 33.70265519714989 39.0 33.0 41.0 10.0 41.0 27 33.64321685774251 39.0 33.0 41.0 10.0 41.0 28 33.52459947614355 39.0 33.0 41.0 9.0 41.0 29 33.509949817430034 39.0 33.0 41.0 9.0 41.0 30 33.357921767684275 38.0 33.0 40.0 8.0 41.0 31 33.349470516987296 38.0 33.0 40.0 8.0 41.0 32 33.23388925853359 38.0 33.0 40.0 8.0 41.0 33 33.18483453636041 38.0 33.0 40.0 2.0 41.0 34 33.108338687810274 38.0 32.0 40.0 2.0 41.0 35 33.04765775049839 38.0 32.0 40.0 2.0 41.0 36 32.94988431476907 38.0 32.0 40.0 2.0 41.0 37 32.85813557694106 38.0 32.0 40.0 2.0 41.0 38 32.770523548973735 38.0 32.0 40.0 2.0 41.0 39 32.6665925968426 38.0 32.0 40.0 2.0 41.0 40 32.597356119366744 38.0 32.0 40.0 2.0 41.0 41 32.491373929725384 38.0 32.0 40.0 2.0 41.0 42 32.3437868995312 38.0 31.0 40.0 2.0 41.0 43 32.24953331255997 38.0 31.0 40.0 2.0 41.0 44 32.10334325404058 38.0 31.0 40.0 2.0 41.0 45 31.961413848183064 38.0 31.0 40.0 2.0 41.0 46 31.831816909538556 38.0 31.0 40.0 2.0 41.0 47 31.673272194557864 38.0 31.0 40.0 2.0 41.0 48 31.513242067898467 38.0 31.0 40.0 2.0 41.0 49 31.335295708187292 38.0 30.0 40.0 2.0 41.0 50 31.12239779552988 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5354014322011409 1101 2 0.4089757150762203 1101 3 0.5738432012679482 1101 4 0.7809614517936083 1101 5 0.742645700118203 1101 6 0.5426636042772159 1101 7 0.7129285463020096 1101 8 0.6837095711801027 1101 9 1.0689379502885785 1101 10 1.0649896610154528 1101 11 1.0425770435890058 1101 12 1.0532433176665634 1101 13 1.0008437853859533 1101 14 1.3024448678736533 1101 15 1.2616949086526006 1101 16 1.248394634296183 1101 17 1.3456239154310552 1101 18 1.4370522668091112 1101 19 1.1828711134565637 1101 20 1.2733097209287223 1101 21 1.325111620140369 1101 22 1.2071662991818002 1101 23 1.4054696399824138 1101 24 1.4372635116345123 1101 25 1.35599690842335 1101 26 1.3812997132017912 1101 27 1.3505007085914826 1101 28 1.4475095771477484 1101 29 1.2561931304583354 1101 30 1.539702381370276 1101 31 1.4054509539499236 1101 32 1.3334778517280057 1101 33 1.3440195346662236 1101 34 1.4096333357406436 1101 35 1.2918048116135097 1101 36 1.449872887667965 1101 37 1.5615195884492081 1101 38 1.5740973901177568 1101 39 1.5862780872295588 1101 40 1.5072190797206275 1101 41 1.6266741031266534 1101 42 1.6377796411973407 1101 43 1.4453306722134798 1101 44 1.4993884921238774 1101 45 1.4163577407889036 1101 46 1.3492054843935293 1101 47 1.5802468012487303 1101 48 1.528784143879534 1101 49 1.5160573078383734 1101 50 1.557700690281436 1102 1 1.0019737685604895 1102 2 0.7538214724506744 1102 3 0.8887888026809954 1102 4 1.0947656666202263 1102 5 1.071709345857009 1102 6 0.993492653553723 1102 7 1.1005780277386208 1102 8 1.2013828944844533 1102 9 1.2792917262457664 1102 10 1.363716961777797 1102 11 1.209931591063281 1102 12 1.2788449186197965 1102 13 1.2657590456182248 1102 14 1.4886764379551423 1102 15 1.3702428316910016 1102 16 1.4825184318403686 1102 17 1.7547860677647833 1102 18 1.650907713465557 1102 19 1.5446247563325741 1102 20 1.4898737673479658 1102 21 1.5413653464684458 1102 22 1.5663707854763231 1102 23 1.5491982726617977 1102 24 1.3673489201240727 1102 25 1.5994687187583594 1102 26 1.6494188971179824 1102 27 1.4780903477138096 1102 28 1.6599459242243242 1102 29 1.524184787462616 1102 30 1.6248370265774241 1102 31 1.7365821107502768 1102 32 1.7434017113914777 1102 33 1.7414112155455257 1102 34 1.875400857081715 1102 35 1.8003466277293754 1102 36 1.8688912261207946 1102 37 1.8100449462706578 1102 38 1.906119162816438 1102 39 2.0158810212051606 1102 40 1.945713803450083 1102 41 1.771753581545731 1102 42 1.8341556109821724 1102 43 1.648422046143935 1102 44 1.6381587734623366 1102 45 1.6845280460023915 1102 46 1.6647695153382145 1102 47 1.6277264660256598 1102 48 1.5437592298462093 1102 49 1.5011804576619028 1102 50 1.4905252331465064 1103 1 0.4158625529911646 1103 2 0.4022679538218341 1103 3 0.43642953821771613 1103 4 0.4249297292831784 1103 5 0.46148568974069093 1103 6 0.38295328834468023 1103 7 0.4007442457086299 1103 8 0.5718842365652819 1103 9 0.6203628504142387 1103 10 0.5229075244661985 1103 11 0.5567334873883922 1103 12 0.585962725003391 1103 13 0.5481272586403136 1103 14 0.5949714417889709 1103 15 0.6633661131736446 1103 16 0.43373886494290304 1103 17 0.692452172184062 1103 18 0.6584518971332471 1103 19 0.7361694365277884 1103 20 0.6492479904135706 1103 21 0.5411209903711267 1103 22 0.6148237322611436 1103 23 0.6033439806547918 1103 24 0.5836718668261796 1103 25 0.6651790928901278 1103 26 0.6064376492028103 1103 27 0.5872823034603343 1103 28 0.6687828353825935 1103 29 0.6099435770884583 1103 30 0.8079262753202059 1103 31 0.7259878148995398 1103 32 0.8442894167476567 1103 33 0.7361738244850855 1103 34 0.6612705178946428 1103 35 0.6915536600694807 1103 36 0.6387256460648416 1103 37 0.6033312079415794 1103 38 0.6018607033885104 1103 39 0.748383817851817 1103 40 0.7140187521457051 1103 41 0.6667946447885882 1103 42 0.7390855092016366 1103 43 0.7333870210087525 1103 44 0.675932724432478 1103 45 0.6033049927326104 1103 46 0.6608395182090803 1103 47 0.6260827081871732 1103 48 0.6142879611244823 1103 49 0.5410388194285929 1103 50 0.4317760389760785 1104 1 0.6630514717632927 1104 2 0.7099631631258099 1104 3 0.6859626441686615 1104 4 0.6555297025221876 1104 5 0.6562302806033173 1104 6 0.5848572830753795 1104 7 0.6383913610039684 1104 8 0.5910808425479033 1104 9 0.7526692318613613 1104 10 0.5915200516147436 1104 11 0.7086122918136155 1104 12 0.7091763911772304 1104 13 0.7529503471720389 1104 14 0.8467221383262142 1104 15 0.804868051707011 1104 16 0.9049685298788717 1104 17 0.9870137734265825 1104 18 0.848185622027259 1104 19 0.884994891690873 1104 20 0.8693267669965508 1104 21 0.8648681233637703 1104 22 0.9182446375893818 1104 23 0.8900989421988754 1104 24 0.8077333779438902 1104 25 0.8756400507016835 1104 26 0.8183299963097781 1104 27 0.9291359437922466 1104 28 0.8177769494806455 1104 29 0.9007002360344103 1104 30 0.8910809460894029 1104 31 0.9495513766895556 1104 32 0.9647915157896065 1104 33 0.9710076776180472 1104 34 0.9131047247690418 1104 35 0.8822381085811841 1104 36 0.8517346725943824 1104 37 0.9958271304131898 1104 38 0.9094561835747896 1104 39 0.9452780524398463 1104 40 0.9810175188221208 1104 41 1.0252616407374546 1104 42 0.9087321924876406 1104 43 0.8347346173670473 1104 44 0.8821074599219827 1104 45 0.9490532701322394 1104 46 0.909419566020901 1104 47 0.8941268489909255 1104 48 0.9983796064087755 1104 49 1.0662675625972042 1104 50 0.9778077846177595 1105 1 0.31745019078859826 1105 2 0.39529700512359156 1105 3 0.32297293539579996 1105 4 0.39277075780156423 1105 5 0.4249495040972917 1105 6 0.36585173530774284 1105 7 0.36869275208675845 1105 8 0.3523596804131728 1105 9 0.4615545803979373 1105 10 0.3222358422466769 1105 11 0.40472570062350144 1105 12 0.3454132507925749 1105 13 0.36287943512741805 1105 14 0.4955146462564741 1105 15 0.5135028059094395 1105 16 0.4188419983517022 1105 17 0.4274179358311798 1105 18 0.33203305337498534 1105 19 0.32951069348196427 1105 20 0.47267685814459526 1105 21 0.48486981086508507 1105 22 0.5579262348268941 1105 23 0.44410749176424247 1105 24 0.5642367506297958 1105 25 0.42044233445864876 1105 26 0.5446989330784362 1105 27 0.527348344917776 1105 28 0.610051806766279 1105 29 0.5165233136310903 1105 30 0.6387952948694533 1105 31 0.5116339230706259 1105 32 0.6268721036723903 1105 33 0.6559030963401185 1105 34 0.6289982654742445 1105 35 0.48768254693857926 1105 36 0.6027829461508816 1105 37 0.6888497330019945 1105 38 0.7140032715000331 1105 39 0.6628075037959462 1105 40 0.5560865052696684 1105 41 0.6280135138386029 1105 42 0.6767239292601772 1105 43 0.6950412418843399 1105 44 0.6561486981087441 1105 45 0.6824125662222613 1105 46 0.7569009080150764 1105 47 0.5432777270659166 1105 48 0.6462947226787463 1105 49 0.7176980859605067 1105 50 0.5580044933827963 1106 1 0.17435832103328508 1106 2 0.1407166989134403 1106 3 0.09068583543013631 1106 4 0.18596147785000028 1106 5 0.15193037400943865 1106 6 0.18879262336699298 1106 7 0.1714925872661297 1106 8 0.22729822240575714 1106 9 0.24379642065682106 1106 10 0.24974217741144145 1106 11 0.27408590840757796 1106 12 0.26911422214470093 1106 13 0.31585187589543295 1106 14 0.36041621279165525 1106 15 0.3511434598738177 1106 16 0.1549026739957 1106 17 0.3505133972476031 1106 18 0.16568114041737658 1106 19 0.2750929332523029 1106 20 0.23491617127523057 1106 21 0.35197869085481415 1106 22 0.3897093421687643 1106 23 0.3742389922813203 1106 24 0.2737053724430112 1106 25 0.19021822185654713 1106 26 0.18810123472133 1106 27 0.3747889565510505 1106 28 0.18762617081308974 1106 29 0.18556641345072222 1106 30 0.16896314417981984 1106 31 0.22169520308724344 1106 32 0.35755211157449907 1106 33 0.293120871445943 1106 34 0.33203305989031406 1106 35 0.24495813436794833 1106 36 0.3482421049947675 1106 37 0.34099121260487664 1106 38 0.37519180197723756 1106 39 0.38112005087394607 1106 40 0.42975863983662776 1106 41 0.3986607031931868 1106 42 0.23322941359476346 1106 43 0.25154581636177653 1106 44 0.3211375881842429 1106 45 0.36591159022986375 1106 46 0.4292496326057531 1106 47 0.37162293915305256 1106 48 0.40694565268355376 1106 49 0.425676589220469 1106 50 0.41163713787465284 1107 1 0.2659926651175617 1107 2 0.29228932137874963 1107 3 0.23527343400665046 1107 4 0.19496921090068042 1107 5 0.21661723453036785 1107 6 0.22774341248216246 1107 7 0.25621054334467885 1107 8 0.2698969975272547 1107 9 0.2429184998963123 1107 10 0.17563263856632716 1107 11 0.16830321974851614 1107 12 0.22155529346960634 1107 13 0.2630992751655725 1107 14 0.1708182677842771 1107 15 0.21198279102198114 1107 16 0.26714559404786087 1107 17 0.2537750325388828 1107 18 0.26289011924286854 1107 19 0.28626634825711506 1107 20 0.36045884002765405 1107 21 0.3480315686545481 1107 22 0.10154400913233275 1107 23 0.027251775919481247 1107 24 0.11025088000834415 1107 25 0.01751103855618652 1107 26 0.34690156593232047 1107 27 0.3228818589821998 1107 28 0.33763030312548636 1107 29 0.29039997441522303 1107 30 0.28748438445183666 1107 31 0.19492068791107187 1107 32 0.20737877429739626 1107 33 0.28157118871736486 1107 34 0.3613576105134513 1107 35 0.3553254913506265 1107 36 0.21103577025301945 1107 37 0.21771259645332464 1107 38 0.18228793253058484 1107 39 0.2214577096093322 1107 40 0.2648009023257316 1107 41 0.44704568446960025 1107 42 0.27667749256135465 1107 43 0.18314117884158776 1107 44 0.46064989483654983 1107 45 0.5377935034226837 1107 46 0.376038415997094 1107 47 0.41628950998285674 1107 48 0.3908169281342566 1107 49 0.473248932074668 1107 50 0.4081362234375163 1108 1 -0.0032410432460743266 1108 2 -0.08043383988943731 1108 3 0.04240455030212331 1108 4 -0.09207623161584877 1108 5 -0.06507586405171395 1108 6 -0.246076770408294 1108 7 -0.2901946993089837 1108 8 -0.17823517552944068 1108 9 -0.15539945380481868 1108 10 -0.23866518612311438 1108 11 -0.16427884873223064 1108 12 -0.27679407464427186 1108 13 -0.2267000353527422 1108 14 -0.3372261560310932 1108 15 -0.2377870051128994 1108 16 -0.1359413143041195 1108 17 -0.19266941494508671 1108 18 -0.2989368793709062 1108 19 -0.11411532273392311 1108 20 -0.3301897003394956 1108 21 -0.33673141532513995 1108 22 -0.3678557064876884 1108 23 -0.47435514637199816 1108 24 -0.2827489986752312 1108 25 -0.24119884153680005 1108 26 -0.39887837677540716 1108 27 -0.3628052707127907 1108 28 -0.5059354364022042 1108 29 -0.4156312954882537 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2216 47 -1.3264440018357142 2216 48 -1.5584640303918427 2216 49 -1.599631560509284 2216 50 -1.6758313808905605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 187220.0 3 9333.0 4 16755.0 5 12777.0 6 10642.0 7 9117.0 8 8087.0 9 7402.0 10 7157.0 11 7238.0 12 7280.0 13 7735.0 14 8039.0 15 8612.0 16 9185.0 17 9895.0 18 10955.0 19 12191.0 20 14455.0 21 18085.0 22 21455.0 23 24535.0 24 27407.0 25 30595.0 26 34202.0 27 38560.0 28 43961.0 29 51101.0 30 59341.0 31 71182.0 32 86721.0 33 107050.0 34 135622.0 35 176758.0 36 233197.0 37 317732.0 38 495200.0 39 817902.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54847183600176 16.420192354976308 12.282482308767333 25.748853500254604 2 16.588228239166504 18.569045752347364 38.29488512495839 26.54784088352774 3 20.458555079028834 22.170973387978073 27.44229111904523 29.928180413947864 4 13.597267809500602 14.737372852743993 35.07493660089406 36.59042273686134 5 15.334093135886201 36.1004043537194 33.31999659865673 15.24550591173768 6 35.34438381877043 33.813521234509714 16.353580856510415 14.48851409020944 7 30.913269322379115 30.084218151463272 19.707631013237542 19.294881512920067 8 27.717280120628097 34.053173164054776 18.82841167192279 19.401135043394333 9 27.002542508354498 15.519418978480026 17.9070170185664 39.57102149459907 10 15.258858264595165 28.275409336974 32.601933865479324 23.863798532951506 11 37.35305273546765 21.714895349825508 21.60408629786133 19.327965616845514 12 24.394985246714327 23.52050854193514 28.78211729869131 23.302388912659218 13 29.346948755998483 20.362368228509737 24.61730409937423 25.67337891611755 14 22.97611451029545 19.852529809289056 25.541571890528648 31.62978378988685 15 25.05997356215684 27.603379070971144 22.188029644890545 25.14861772198147 16 25.11738265540436 26.57576937812883 23.89702803989678 24.409819926570027 17 24.151997559148466 26.485998405327248 25.20746920867061 24.15453482685368 18 24.18195513760813 26.296497752122995 25.52874665990057 23.992800450368303 19 24.937538082183615 25.87780523554098 25.622248210355437 23.562408471919973 20 25.174305663934877 25.452877518471006 25.4775562821728 23.895260535421322 21 25.31308976461284 25.213699209856195 25.40657969913816 24.06663132639281 22 25.126486301282945 25.051298808420995 25.26854942070733 24.55366546958872 23 24.824910770386268 25.136124125029617 25.19197886591088 24.84698623867324 24 24.529309243272017 25.189624532322934 25.438111184215117 24.84295504018993 25 24.823775709521076 25.151462949515103 25.38749674422471 24.637264596739115 26 24.647974231888263 25.386336086301476 25.437254311631598 24.52843537017867 27 24.71061798592212 25.520846385885193 25.22515949359218 24.543376134600507 28 24.645223888268767 25.398276362071844 25.246739384955326 24.709760364704067 29 24.64935766200582 25.559922093933345 25.30839933109158 24.482320912969254 30 24.856431324651428 25.520771536887523 25.236267309614075 24.386529828846978 31 24.83777025604072 25.171459236724118 25.256998556266286 24.733771950968876 32 24.734447766798645 25.527362060632047 25.103138165815214 24.63505200675409 33 24.673639180044606 25.24282006196098 25.23907753749502 24.844463220499392 34 24.874164439509656 25.331647385782286 25.319246981484707 24.474941193223355 35 24.92424381787053 25.363842178479473 25.274526956006543 24.437387047643455 36 24.849290451166045 25.373915409815478 25.281017368349772 24.495776770668705 37 24.97328586267871 25.124991079428767 25.234194730122983 24.66752832776954 38 24.885733241826898 25.257330134836675 25.276866166764133 24.58007045657229 39 24.956287510042056 25.534025015628025 25.107857972228892 24.401829502101023 40 25.02756962022908 25.178857613893374 25.27787671582628 24.515696050051268 41 24.844324191303972 25.357968736720466 25.305706823350928 24.492000248624638 42 24.965585169451618 25.362358594765013 25.05865821958707 24.613398016196296 43 24.687157614339963 25.118212736944752 25.35785693333439 24.836772715380896 44 24.72197575901331 25.379467017937518 25.195829905926807 24.70272731712236 45 24.866920417331485 25.33001960839026 25.176646736966003 24.626413237312256 46 24.841019201988686 25.016107348502143 25.197790629835374 24.945082819673793 47 24.745131650667933 25.30695024585509 25.188834529235145 24.75908357424183 48 24.67971756487374 25.3841248984973 25.26671310715736 24.669444429471596 49 24.857587300902832 25.204083120948223 25.16597111731585 24.7723584608331 50 24.949743676898454 25.26675485568033 25.197378934578634 24.586122532842587 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1292.0 1 893.0 2 494.0 3 522.5 4 551.0 5 568.0 6 585.0 7 583.5 8 582.0 9 653.5 10 725.0 11 913.5 12 1102.0 13 1503.0 14 1904.0 15 2510.5 16 3117.0 17 3276.5 18 3436.0 19 3526.5 20 3617.0 21 3942.0 22 4267.0 23 5237.0 24 6207.0 25 7813.5 26 9420.0 27 12246.0 28 15072.0 29 19826.0 30 24580.0 31 32507.5 32 40435.0 33 52281.0 34 64127.0 35 78555.0 36 92983.0 37 108337.5 38 123692.0 39 140329.5 40 156967.0 41 171200.5 42 185434.0 43 198452.5 44 211471.0 45 224579.0 46 237687.0 47 248222.5 48 258758.0 49 260482.5 50 262207.0 51 261329.5 52 260452.0 53 254499.0 54 248546.0 55 237330.5 56 226115.0 57 209721.5 58 193328.0 59 175769.0 60 158210.0 61 138721.5 62 119233.0 63 102428.0 64 85623.0 65 71545.5 66 57468.0 67 47140.0 68 36812.0 69 30329.0 70 23846.0 71 18732.0 72 13618.0 73 11281.5 74 8945.0 75 6930.5 76 4916.0 77 3926.5 78 2937.0 79 2308.0 80 1679.0 81 1326.5 82 974.0 83 745.0 84 516.0 85 417.0 86 318.0 87 243.5 88 169.0 89 134.5 90 100.0 91 77.0 92 54.0 93 45.0 94 36.0 95 26.0 96 16.0 97 18.0 98 20.0 99 29.0 100 38.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08546030486125221 2 0.09357522995193492 3 0.10058069262787585 4 0.10197544537783694 5 0.09462129451440573 6 0.05762864771430139 7 0.05322249698146976 8 0.05864301335063672 9 0.05987927146992041 10 0.044727184777161304 11 0.04783367953843828 12 0.05781884127111426 13 0.050401292555412106 14 0.05189114208377963 15 0.05151075497015387 16 0.05531462610641139 17 0.05334929268601168 18 0.0540783679871277 19 0.06212989522553945 20 0.06888176649239654 21 0.07829634755463388 22 0.0811809498329625 23 0.05896000261199152 24 0.07604572379901486 25 0.0821002186908914 26 0.08140284231591086 27 0.07480946567973117 28 0.09430430525305095 29 0.08495312204308456 30 0.08022998204889813 31 0.05531462610641139 32 0.05163755067469579 33 0.05480744328824372 34 0.049386926919076764 35 0.0571531638222692 36 0.055853507850714544 37 0.058833206907449594 38 0.04859445376568978 39 0.05363458302123098 40 0.05559991644163071 41 0.042254668538593915 42 0.03943346411253626 43 0.03981385122616201 44 0.037373033913730105 45 0.02944830237986028 46 0.0266587968799381 47 0.03150873257866643 48 0.026119915135634948 49 0.025961420504957554 50 0.026912388289021934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3154681.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.34733170656884 #Duplication Level Percentage of deduplicated Percentage of total 1 78.36257997554587 50.42422927068979 2 12.27691428602855 15.799733517923856 3 4.235827901257578 8.176926690424821 4 1.881079245484602 4.841697207021596 5 0.9841062114271868 3.166230441059997 6 0.6041973833703219 2.332709366638263 7 0.3637437298809531 1.6384156909983985 8 0.24703177345530938 1.2716668374872608 9 0.18313448034665664 1.0605793638398784 >10 0.8188310519705563 8.944688617823203 >50 0.034104504445363545 1.4779254822238712 >100 0.008301267709329744 0.7992113575024188 >500 1.4818907768351488E-4 0.0659861563667469 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.339785227095862E-5 2 0.0 0.0 0.0 0.0 6.339785227095862E-5 3 0.0 0.0 0.0 0.0 6.339785227095862E-5 4 0.0 0.0 0.0 0.0 6.339785227095862E-5 5 0.0 9.509677840643792E-5 0.0 0.0 6.339785227095862E-5 6 0.0 9.509677840643792E-5 0.0 0.0 1.2679570454191723E-4 7 0.0 9.509677840643792E-5 0.0 0.0 1.2679570454191723E-4 8 0.0 9.509677840643792E-5 0.0 0.0 1.5849463067739656E-4 9 0.0 9.509677840643792E-5 0.0 0.0 1.5849463067739656E-4 10 0.0 9.509677840643792E-5 0.0 0.0 1.5849463067739656E-4 11 0.0 9.509677840643792E-5 0.0 0.0 1.5849463067739656E-4 12 0.0 9.509677840643792E-5 0.0 0.0 1.5849463067739656E-4 13 0.0 9.509677840643792E-5 0.0 0.0 1.5849463067739656E-4 14 0.0 9.509677840643792E-5 0.0 0.0 1.9019355681287585E-4 15 0.0 9.509677840643792E-5 0.0 0.0 2.2189248294835517E-4 16 0.0 9.509677840643792E-5 0.0 3.169892613547931E-5 2.2189248294835517E-4 17 0.0 9.509677840643792E-5 0.0 3.169892613547931E-5 2.2189248294835517E-4 18 0.0 9.509677840643792E-5 0.0 3.169892613547931E-5 2.5359140908383447E-4 19 0.0 9.509677840643792E-5 0.0 3.169892613547931E-5 2.852903352193138E-4 20 0.0 9.509677840643792E-5 0.0 3.169892613547931E-5 2.852903352193138E-4 21 0.0 9.509677840643792E-5 0.0 3.169892613547931E-5 3.169892613547931E-4 22 0.0 9.509677840643792E-5 0.0 6.339785227095862E-5 3.486881874902724E-4 23 0.0 1.2679570454191723E-4 0.0 9.509677840643792E-5 4.12086039761231E-4 24 0.0 1.2679570454191723E-4 0.0 1.5849463067739656E-4 4.4378496589671034E-4 25 0.0 1.2679570454191723E-4 0.0 2.5359140908383447E-4 4.4378496589671034E-4 26 0.0 1.2679570454191723E-4 0.0 3.486881874902724E-4 4.4378496589671034E-4 27 0.0 1.2679570454191723E-4 0.0 6.022795965741069E-4 4.4378496589671034E-4 28 0.0 1.5849463067739656E-4 0.0 0.0012679570454191724 4.754838920321896E-4 29 0.0 1.5849463067739656E-4 0.0 0.0025993119431093033 4.754838920321896E-4 30 0.0 1.5849463067739656E-4 0.0 0.0046914410680509375 4.754838920321896E-4 31 3.169892613547931E-5 1.5849463067739656E-4 0.0 0.010682538107656526 5.071828181676689E-4 32 3.169892613547931E-5 1.5849463067739656E-4 0.0 0.01743440937451362 5.071828181676689E-4 33 3.169892613547931E-5 1.5849463067739656E-4 0.0 0.025834624800415637 5.071828181676689E-4 34 3.169892613547931E-5 1.5849463067739656E-4 0.0 0.03895798022050407 5.388817443031483E-4 35 3.169892613547931E-5 1.5849463067739656E-4 0.0 0.05493423899278564 5.388817443031483E-4 36 3.169892613547931E-5 1.5849463067739656E-4 0.0 0.07595062702060842 5.388817443031483E-4 37 3.169892613547931E-5 1.5849463067739656E-4 0.0 0.10308490779257871 5.388817443031483E-4 38 3.169892613547931E-5 1.5849463067739656E-4 0.0 0.13291359728606475 5.388817443031483E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3405 0.0 21.715904 1 GGTATCA 1930 0.0 19.498203 1 TATCAAC 4195 0.0 16.996326 2 TCAACGC 4280 0.0 16.761082 4 ACCGTAT 210 9.094947E-12 16.759596 8 ATCAACG 4290 0.0 16.61995 3 CAACGCA 4470 0.0 15.948667 5 AACGCAG 4620 0.0 15.473324 6 TATACTG 640 0.0 14.783435 5 GTACATG 8390 0.0 14.662419 1 TACATGG 8390 0.0 14.189835 2 GCGATAC 190 1.7565253E-7 13.894146 14 ACATGGG 8775 0.0 13.140931 3 GTATAGC 610 0.0 12.987587 1 TACGTCA 170 1.1838574E-5 12.944322 4 ATTATAC 415 0.0 12.726388 3 TGTACTG 1080 0.0 12.631496 5 GTATTAC 315 3.092282E-11 12.575282 1 TATACAG 910 0.0 12.573287 5 GTAATAC 265 4.9330993E-9 12.456253 3 >>END_MODULE