FastQCFastQC Report
Mon 6 Feb 2017
SRR2077109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154681
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG1502.1827873E-1120.52977437
GTATCAA36000.020.475491
GGTATCA20700.019.6649741
TAGATCG1102.7963288E-618.0020185
TACCGAT2650.016.605377
GTACCGA3050.016.5914976
TTATACT5250.015.9263524
TACGACT1301.3682646E-515.2332014
TATCAAC47450.015.2104982
CGATCAA1603.8228427E-715.12717410
TCAACGC49450.014.4613584
ATCAACG49600.014.41762353
GTACATG77950.014.2267731
TTAGTCG1251.6759579E-414.07644539
CAACGCA50850.013.9327325
TACATGG79550.013.7197842
AACGCAG53050.013.4789346
CTTAGAC5950.013.3130493
ACCGTCG1000.00875810613.2014798
TATACCG1353.2067802E-413.0384985
Produced by FastQC (version 0.11.5)