FastQCFastQC Report
Mon 6 Feb 2017
SRR2077108_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077108_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268200
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.023.0928611
GTATCAA42150.020.3104131
CGTGCCG2700.018.73777210
TATCAAC51450.016.38252
TACCGAT3400.016.1734147
GTCTAGG7400.015.7619851
GTATACG1401.5826645E-615.71951
TCAACGC52850.015.6567984
ATCAACG53450.015.6462153
CAACGCA53500.015.5055735
TATAGCG1004.940188E-415.402755
AACGCAG55750.014.9529996
GTATTAC3300.014.6715341
GTACATG86850.014.3674591
GGACCGT2950.014.1665976
TACATGG87100.013.8714212
GCGTAAC1150.001426474713.38918411
CGAATCG2805.820766E-1113.3556437
CTAGGAC7950.013.2876313
TATACGA1000.00874549113.204382
Produced by FastQC (version 0.11.5)