##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077108_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3268200 Sequences flagged as poor quality 0 Sequence length 50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.198783122207942 31.0 30.0 34.0 23.0 34.0 2 29.226848724068294 31.0 30.0 34.0 22.0 34.0 3 29.28849152438651 31.0 30.0 34.0 23.0 34.0 4 32.67388195336883 35.0 33.0 37.0 25.0 37.0 5 32.63975613487547 37.0 33.0 37.0 25.0 37.0 6 32.71441313261122 37.0 35.0 37.0 25.0 37.0 7 32.52222783183404 37.0 33.0 37.0 23.0 37.0 8 32.466706137935255 37.0 33.0 37.0 23.0 37.0 9 33.808840952206104 38.0 34.0 39.0 22.0 39.0 10 33.743831160883666 38.0 34.0 39.0 22.0 39.0 11 33.816084388960284 38.0 34.0 39.0 22.0 39.0 12 33.715678661036655 38.0 34.0 39.0 22.0 39.0 13 33.670909063092836 38.0 34.0 39.0 19.0 39.0 14 34.61959366011872 39.0 34.0 41.0 18.0 41.0 15 34.68655253656447 39.0 34.0 41.0 19.0 41.0 16 34.665295881525 39.0 34.0 41.0 19.0 41.0 17 34.51191450951594 39.0 34.0 41.0 18.0 41.0 18 34.49720916712563 39.0 34.0 41.0 18.0 41.0 19 34.627410195214495 39.0 34.0 41.0 18.0 41.0 20 34.61343155253657 39.0 34.0 41.0 18.0 41.0 21 34.56803347408359 39.0 34.0 41.0 18.0 41.0 22 34.493326601799154 39.0 34.0 41.0 18.0 41.0 23 34.45887858760174 39.0 34.0 41.0 18.0 41.0 24 34.46892693225629 39.0 34.0 41.0 18.0 41.0 25 34.38598463986292 39.0 34.0 41.0 18.0 41.0 26 34.33845480692736 39.0 34.0 41.0 18.0 41.0 27 34.281917569304206 39.0 34.0 41.0 17.0 41.0 28 34.170999326846584 39.0 34.0 41.0 17.0 41.0 29 34.159049017807966 39.0 34.0 41.0 16.0 41.0 30 34.00406003304571 39.0 33.0 41.0 16.0 41.0 31 34.003108132917205 39.0 33.0 41.0 16.0 41.0 32 33.86727250474267 39.0 33.0 41.0 15.0 41.0 33 33.81661312037207 39.0 33.0 41.0 15.0 41.0 34 33.745393488770574 38.0 33.0 41.0 13.0 41.0 35 33.67821277767578 38.0 33.0 41.0 9.0 41.0 36 33.5890505477021 38.0 33.0 40.0 9.0 41.0 37 33.501828835444584 38.0 33.0 40.0 9.0 41.0 38 33.41591763049997 38.0 33.0 40.0 8.0 41.0 39 33.304955939049016 38.0 33.0 40.0 8.0 41.0 40 33.2417602961875 38.0 33.0 40.0 7.0 41.0 41 33.13979835995349 38.0 33.0 40.0 2.0 41.0 42 32.99023437978092 38.0 32.0 40.0 2.0 41.0 43 32.9086940823695 38.0 32.0 40.0 2.0 41.0 44 32.768652469249126 38.0 32.0 40.0 2.0 41.0 45 32.626422495563304 38.0 32.0 40.0 2.0 41.0 46 32.48941252065357 38.0 32.0 40.0 2.0 41.0 47 32.336554678416256 38.0 32.0 40.0 2.0 41.0 48 32.17467841625359 38.0 31.0 40.0 2.0 41.0 49 32.00446208922342 38.0 31.0 40.0 2.0 41.0 50 31.796665748730188 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.849673516800177 1101 2 1.8889132929733883 1101 3 1.9791475926006363 1101 4 2.1633321759493 1101 5 2.195893363427082 1101 6 2.1838921968437006 1101 7 2.1595843956537877 1101 8 2.213037348312696 1101 9 2.4028964255481213 1101 10 2.4749966758687023 1101 11 2.4982872483408727 1101 12 2.6948089194385716 1101 13 2.5919718816109807 1101 14 3.142263321663883 1101 15 3.040927978361964 1101 16 3.1574385359274046 1101 17 3.2645793817440207 1101 18 3.230893812903645 1101 19 3.201689626304109 1101 20 3.162165057766714 1101 21 3.1507161832586448 1101 22 3.2229642882122818 1101 23 3.2208805097986186 1101 24 3.2702694719684544 1101 25 3.2728898036716174 1101 26 3.3368142261400493 1101 27 3.2981708055180263 1101 28 3.3002682425090555 1101 29 3.2717192946971423 1101 30 3.5076626748930124 1101 31 3.4669264795373707 1101 32 3.4617762721775023 1101 33 3.52222653407064 1101 34 3.5243949425952934 1101 35 3.4802666400206306 1101 36 3.5354643053387207 1101 37 3.705394856401604 1101 38 3.598651485284769 1101 39 3.6569545498535234 1101 40 3.6555289753401183 1101 41 3.666016050282451 1101 42 3.701425995408215 1101 43 3.655476039145249 1101 44 3.5618093602874836 1101 45 3.6765134540517934 1101 46 3.6122660759855947 1101 47 3.6534161170914743 1101 48 3.778825104677317 1101 49 3.775097467076634 1101 50 3.927370244398009 1102 1 1.2361000389937402 1102 2 1.2071593499783795 1102 3 1.1628965605989592 1102 4 1.2820182506640805 1102 5 1.280139456792753 1102 6 1.3596770171862858 1102 7 1.3488385311465123 1102 8 1.4804266656872542 1102 9 1.5107111914067275 1102 10 1.5692031004445255 1102 11 1.5478995258455939 1102 12 1.6937747788981241 1102 13 1.700760988949142 1102 14 2.067438565886455 1102 15 1.844565864480309 1102 16 1.9065894962058607 1102 17 2.003154552817456 1102 18 2.0760259198126008 1102 19 2.0447315287804884 1102 20 1.9279993322000237 1102 21 1.940086958111884 1102 22 1.9511711398478653 1102 23 1.9439734277361254 1102 24 1.8297275623625353 1102 25 2.028635325960714 1102 26 2.05001908863251 1102 27 1.9001308779256831 1102 28 2.1003523176715717 1102 29 1.966881951707549 1102 30 2.077078957633667 1102 31 2.051923403138595 1102 32 2.2360357084871367 1102 33 2.178338800168085 1102 34 2.2362126629374544 1102 35 2.236230237275471 1102 36 2.272058977857313 1102 37 2.222295621686989 1102 38 2.1794200538289346 1102 39 2.33628177387952 1102 40 2.320217282315525 1102 41 2.230895892491354 1102 42 2.2258173283052187 1102 43 2.1331514723007743 1102 44 2.0956914547197343 1102 45 2.1424232660171683 1102 46 2.070527935627659 1102 47 2.065947201908827 1102 48 2.1254094108371007 1102 49 2.1694489244269803 1102 50 2.1705029772810356 1103 1 0.43265596305905163 1103 2 0.5313658249890061 1103 3 0.4440425543327464 1103 4 0.5380983713432883 1103 5 0.5415823234580301 1103 6 0.5087474397105964 1103 7 0.47896060387454753 1103 8 0.5499743664108792 1103 9 0.6822209356253452 1103 10 0.6184960223475571 1103 11 0.5605923547695824 1103 12 0.5667347173972459 1103 13 0.5791657849178264 1103 14 0.6907235632813382 1103 15 0.6455104997601637 1103 16 0.6427125587232254 1103 17 0.8158251627707784 1103 18 0.629674137378899 1103 19 0.6675586469954737 1103 20 0.7488375428683796 1103 21 0.5185460531417192 1103 22 0.5349624491336158 1103 23 0.6268317821852207 1103 24 0.514442207296419 1103 25 0.6621091149300398 1103 26 0.6243762384572733 1103 27 0.6704363171606715 1103 28 0.6934786913574982 1103 29 0.7487684487668247 1103 30 0.8953712284511752 1103 31 0.701530380901815 1103 32 0.8266142552580718 1103 33 0.6455933986047029 1103 34 0.6601728809156242 1103 35 0.6512502711226276 1103 36 0.8361669016686974 1103 37 0.8632003789466722 1103 38 0.8135582345365151 1103 39 0.8184881621819713 1103 40 0.7783955376850429 1103 41 0.8256543347324836 1103 42 0.8421712358634679 1103 43 0.8165022053332578 1103 44 0.81879217788142 1103 45 0.7156990796998741 1103 46 0.7915794038990498 1103 47 0.8406465974289503 1103 48 0.7829283647029328 1103 49 0.9035708279966457 1103 50 0.8684205390935169 1104 1 0.3972365197051033 1104 2 0.4731940151328189 1104 3 0.41225527348141 1104 4 0.3816436759779691 1104 5 0.565576477174659 1104 6 0.47996678334379084 1104 7 0.4450821721650158 1104 8 0.5553261269010576 1104 9 0.5788990392023905 1104 10 0.4388047369219237 1104 11 0.48927460792371136 1104 12 0.4949981573992943 1104 13 0.4652886777398635 1104 14 0.5433620414443112 1104 15 0.48904076349935366 1104 16 0.5684830577439968 1104 17 0.6297266113256157 1104 18 0.5344923191864837 1104 19 0.5865951000242902 1104 20 0.5816698822394883 1104 21 0.5859212586957696 1104 22 0.570865494199829 1104 23 0.6141390541939131 1104 24 0.5998676534056813 1104 25 0.6597936720617525 1104 26 0.5874960404335852 1104 27 0.6647276560417268 1104 28 0.5675593457693751 1104 29 0.6803772248922684 1104 30 0.63783915024365 1104 31 0.6920646051380928 1104 32 0.7060549484824463 1104 33 0.6997357642173156 1104 34 0.6768649324941549 1104 35 0.6549199775659318 1104 36 0.7126277875090423 1104 37 0.768606749158451 1104 38 0.8428728232607199 1104 39 0.7922861060565793 1104 40 0.8365871688763775 1104 41 0.7406157933122444 1104 42 0.6765551193923685 1104 43 0.8697755025630016 1104 44 0.9109902632881344 1104 45 0.8724731706186262 1104 46 0.8031959111225078 1104 47 0.7831268706338008 1104 48 0.8393022239996668 1104 49 0.8981946989238452 1104 50 0.9157821036824494 1105 1 0.4281116067862989 1105 2 0.5449108557034457 1105 3 0.3795623999441631 1105 4 0.5061479084839249 1105 5 0.4512058190051249 1105 6 0.35888517862060354 1105 7 0.46673183977051735 1105 8 0.5011928021327847 1105 9 0.5255003898976938 1105 10 0.46892971666297 1105 11 0.46963739731292975 1105 12 0.45942328861639226 1105 13 0.5410273680756319 1105 14 0.6631223378840119 1105 15 0.6454775727336681 1105 16 0.6040723246892483 1105 17 0.7013972301032538 1105 18 0.5347720865564796 1105 19 0.46422460134209587 1105 20 0.6009870106256869 1105 21 0.5731088508763094 1105 22 0.674154957440031 1105 23 0.5565628703788477 1105 24 0.6403854851605715 1105 25 0.5483448809720741 1105 26 0.6634655443873569 1105 27 0.6554195763467874 1105 28 0.6911286945183406 1105 29 0.6770535014093468 1105 30 0.6765709822547521 1105 31 0.49076072232288936 1105 32 0.6539504355445942 1105 33 0.7009046365716998 1105 34 0.6845675801685402 1105 35 0.7365175016727008 1105 36 0.7704793454086669 1105 37 0.7641310619323178 1105 38 0.7436066865202662 1105 39 0.8050681300616347 1105 40 0.7833817133757464 1105 41 0.7431122131538004 1105 42 0.7681681898226387 1105 43 0.8072450956746238 1105 44 0.79969826449463 1105 45 0.8568099266535825 1105 46 0.8413021614386196 1105 47 0.8695796027589466 1105 48 0.8277244401426671 1105 49 0.8693734543245384 1105 50 0.9138199664192292 1106 1 0.38833912600293985 1106 2 0.4458185994134922 1106 3 0.32887840229919263 1106 4 0.33947887220516293 1106 5 0.3362836425346387 1106 6 0.27711569813071435 1106 7 0.2872034654884601 1106 8 0.3814974977925729 1106 9 0.42329756203907465 1106 10 0.5403634960302099 1106 11 0.3952842558452616 1106 12 0.5363902945021408 1106 13 0.5394750898758858 1106 14 0.5132968689286628 1106 15 0.5285877068354878 1106 16 0.31521059520051864 1106 17 0.5488953754260919 1106 18 0.26245918110218014 1106 19 0.4154076757497336 1106 20 0.3423258050035045 1106 21 0.5869628791257355 1106 22 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45 0.14128484578889555 1107 46 0.11227475370665019 1107 47 0.12105003707141293 1107 48 0.05305123741033668 1107 49 0.2291994608110386 1107 50 0.22383527675121329 1108 1 -0.0784382341778489 1108 2 -0.1387972131407338 1108 3 -0.0301352913871753 1108 4 -0.13814406303449545 1108 5 -0.14736916497597008 1108 6 -0.21093318247042703 1108 7 -0.2312921226403688 1108 8 -0.1735407866861962 1108 9 -0.34432979166913924 1108 10 -0.27753030310029203 1108 11 -0.2682844188887614 1108 12 -0.3716222540912071 1108 13 -0.35685280257167307 1108 14 -0.43057720436085134 1108 15 -0.2847525733717404 1108 16 -0.24213124840994027 1108 17 -0.34642300929021275 1108 18 -0.2968647682455483 1108 19 -0.2819722686824022 1108 20 -0.338676884235511 1108 21 -0.35620801932117274 1108 22 -0.4297909912720783 1108 23 -0.5140559675674723 1108 24 -0.25705321954592364 1108 25 -0.2619166614702806 1108 26 -0.4474825947165826 1108 27 -0.2456583933855896 1108 28 -0.49520085313944406 1108 29 -0.5577207527294448 1108 30 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13 -0.8059701342083514 2214 14 -0.9261283333422412 2214 15 -0.8697718356652544 2214 16 -0.9128957302493248 2214 17 -0.6632308225443566 2214 18 -0.8890238969820601 2214 19 -0.9732407896454518 2214 20 -0.9280935002047386 2214 21 -0.9785477019961988 2214 22 -0.8826045214481226 2214 23 -0.9595948936344811 2214 24 -1.0834213944545468 2214 25 -1.073012267273242 2214 26 -1.1454679113172972 2214 27 -1.1350117127149488 2214 28 -0.9623714605412133 2214 29 -1.0871897660725693 2214 30 -1.0584114303601098 2214 31 -1.0401801538892954 2214 32 -1.0757030273123576 2214 33 -1.0083382397690102 2214 34 -1.1500034357089959 2214 35 -1.1188566388643295 2214 36 -1.1574634334261233 2214 37 -1.0679279892149793 2214 38 -0.8990790426926765 2214 39 -0.9384185306261585 2214 40 -0.9300451865228396 2214 41 -0.9129192541565772 2214 42 -0.8164924393000561 2214 43 -0.9017223753459973 2214 44 -0.809978708742932 2214 45 -0.8217314379263492 2214 46 -0.9088844687960353 2214 47 -0.8631056934048011 2214 48 -0.8420381790506362 2214 49 -0.8236547329874 2214 50 -0.8707924685186228 2215 1 -0.33140066014415837 2215 2 -0.37918728542937075 2215 3 -0.41595794277180786 2215 4 -0.22508367725302492 2215 5 -0.285098093939105 2215 6 -0.3133438192523528 2215 7 -0.3475656287594546 2215 8 -0.2662220860144515 2215 9 -0.3496298033422818 2215 10 -0.4076488697047722 2215 11 -0.5290598524682153 2215 12 -0.4849046383402751 2215 13 -0.416231131731287 2215 14 -0.5693012724813826 2215 15 -0.5208935649379924 2215 16 -0.5737481377127622 2215 17 -0.3537793868139403 2215 18 -0.3999099845027487 2215 19 -0.40733378489465366 2215 20 -0.4097611942614563 2215 21 -0.45312984277506274 2215 22 -0.4187016418976057 2215 23 -0.5571602758208911 2215 24 -0.5910198337924228 2215 25 -0.5808171958332267 2215 26 -0.6654861751305745 2215 27 -0.6878023036556868 2215 28 -0.5811074148247855 2215 29 -0.6046610598236128 2215 30 -0.5217523464253091 2215 31 -0.5347004031810485 2215 32 -0.6711291595851918 2215 33 -0.5499884574085172 2215 34 -0.6253172211263163 2215 35 -0.7528657845234363 2215 36 -0.6086205415900778 2215 37 -0.657129175917909 2215 38 -0.6631586683883199 2215 39 -0.7205928277538334 2215 40 -0.6902389992077929 2215 41 -0.7278697884263678 2215 42 -0.6213916741776018 2215 43 -0.6635685062921226 2215 44 -0.739948323884235 2215 45 -0.6969042875396312 2215 46 -0.6508244454869576 2215 47 -0.6512372349267288 2215 48 -0.7173640585477585 2215 49 -0.8303845423458718 2215 50 -0.8066794863531435 2216 1 -0.5667415957444462 2216 2 -0.5707666671717746 2216 3 -0.6934562295592492 2216 4 -0.6212417123249452 2216 5 -0.47737025207163697 2216 6 -0.6252807590276461 2216 7 -0.6213832740506575 2216 8 -0.744890664309878 2216 9 -0.829268478622204 2216 10 -0.8160661413518113 2216 11 -0.9467491957119947 2216 12 -0.869000522793435 2216 13 -0.7968622940805474 2216 14 -1.0123402805180106 2216 15 -0.9973653524509061 2216 16 -1.0638424505191892 2216 17 -0.9764617189786193 2216 18 -0.9343361752169841 2216 19 -0.9389181571475262 2216 20 -1.0814798426116639 2216 21 -1.1896404117632713 2216 22 -1.1305217863647599 2216 23 -1.1439243750631576 2216 24 -1.2060812055890935 2216 25 -1.209997483484905 2216 26 -1.038294110210245 2216 27 -1.181112443157538 2216 28 -1.240606383816683 2216 29 -1.4547743544930896 2216 30 -1.2779559682140658 2216 31 -1.3860424920317342 2216 32 -1.215545402884615 2216 33 -1.3139494944601111 2216 34 -1.29452990228787 2216 35 -1.3373763126241798 2216 36 -1.3727529699102945 2216 37 -1.402502381805597 2216 38 -1.4241927514464336 2216 39 -1.4026002731921778 2216 40 -1.5184021692965395 2216 41 -1.6076355928997401 2216 42 -1.4920994361016895 2216 43 -1.6628613772835052 2216 44 -1.790949408522117 2216 45 -1.739175829559759 2216 46 -1.9123675214057272 2216 47 -1.7990393099372923 2216 48 -1.860652830151249 2216 49 -1.9066695497826274 2216 50 -2.1700028610696265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 164624.0 3 8004.0 4 14778.0 5 11334.0 6 9199.0 7 7944.0 8 7255.0 9 6615.0 10 6547.0 11 6647.0 12 6668.0 13 7136.0 14 7814.0 15 8159.0 16 8736.0 17 9440.0 18 10091.0 19 11619.0 20 14383.0 21 18997.0 22 23718.0 23 26630.0 24 28442.0 25 30270.0 26 33397.0 27 38206.0 28 43591.0 29 50395.0 30 59257.0 31 70831.0 32 87524.0 33 108900.0 34 139450.0 35 180518.0 36 240780.0 37 332021.0 38 522166.0 39 906114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.447844426573795 17.00755977291895 12.46829678799991 25.07629901250734 2 16.38036037564627 18.90062549520029 38.91343983157601 25.805574297577433 3 20.02933648934906 23.13539626007236 27.794623746813045 29.040643503765533 4 13.298552542874372 15.205513060561518 35.74242326320919 35.75351113335491 5 14.949306424167286 36.74279686920004 33.366093862745124 14.941802843887553 6 34.525066940490476 34.95355955637115 16.514447083488708 14.006926419649673 7 30.32299640740928 30.867839501353917 20.10108849222932 18.708075599007483 8 27.270058100285677 34.85144580220308 18.90553965726434 18.972956440246904 9 26.769474475724873 15.974593291825666 18.279579110312092 38.97635312213737 10 15.028427692824797 28.73286233943703 32.68840291559487 23.550307052143303 11 36.60039080401359 22.534354538160333 21.99437396509336 18.870880692732715 12 24.00795145120414 24.20367618379327 29.091498021321843 22.69687434368075 13 28.842288299816627 21.10576054712493 25.0598647494436 24.992086403614845 14 22.568248480093114 20.577759014818078 25.917936814610698 30.93605569047811 15 24.49384507529489 28.50545998916268 22.483552681930757 24.517142253611677 16 24.650846390780796 27.5047843205928 24.05643033811852 23.787938950507883 17 23.504934338927168 27.429842379748926 25.463791121404483 23.601432159919423 18 23.50428423253029 26.971683776247584 26.114959729316347 23.409072261905784 19 24.401635828276607 26.53242554681972 26.06762227761256 22.998316347291105 20 24.487026471252847 26.111021635918156 26.051559756739064 23.35039213608993 21 24.671480633028104 25.87565611242156 25.973738048656358 23.47912520589398 22 24.48494751711891 25.552366361347172 25.967060281895098 23.995625839638823 23 24.153424785030545 25.750135169143597 25.914542485361014 24.181897560464847 24 23.84881389792133 25.910762694763168 26.02133633340754 24.219087073907957 25 24.153301858624722 25.68039788343456 26.05042016806723 24.115880089873492 26 23.949557057905388 26.069138102574417 26.166732658592686 23.814572180927506 27 24.118500309120858 26.03281427363642 25.881821213315437 23.96686420392729 28 24.05177153025238 25.997399785306186 25.967109887400596 23.98371879704084 29 24.06501580317521 26.124557990349363 25.902508143511998 23.907918062963425 30 24.103124732052084 26.14292801234704 25.919444138218257 23.834503117382624 31 24.206576995669522 25.796744719315086 25.796316110929972 24.20036217408542 32 23.976166159693953 26.128381654935268 25.84602601970091 24.049426165669864 33 24.103947727337435 25.952735280404088 25.80566254522165 24.137654447036823 34 24.3156773979455 25.85707166009304 25.87715377635625 23.950097165605214 35 24.1697516388213 25.99443664101945 25.86671083391604 23.969100886243208 36 24.1999587312492 25.985077121743426 25.756721326331117 24.058242820676256 37 24.379506801705777 25.61729387232638 25.958136896249684 24.045062429718158 38 24.24259214927316 25.882277669578357 25.947605493414873 23.927524687733616 39 24.340785818877936 26.075311035574956 25.663637975071847 23.920265170475265 40 24.27296670851714 25.78001225205433 26.083803194412425 23.863217845016106 41 24.129430675104285 25.916195027609596 25.939000226215324 24.015374071070795 42 24.329008463325845 25.912182089607978 25.82889260492837 23.929916842137803 43 24.10211735377917 25.73384374460499 25.97722372347186 24.186815178143984 44 24.1790502940359 25.947520438035742 25.799706637509995 24.07372263041836 45 24.181364364669207 25.998803269431185 25.678899742442766 24.140932623456838 46 24.259082254548282 25.749074244957587 25.65811320731618 24.33373029317795 47 24.102453915999885 25.95120081929832 25.76483248887575 24.181512775826047 48 24.01393421525008 26.02736833149035 25.849242490786885 24.109454962472682 49 24.290388235114083 25.805775322509067 25.702388786019252 24.2014476563576 50 24.292134136805046 25.86828781132922 25.736467277013798 24.103110774851935 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2101.0 1 1612.0 2 1123.0 3 1033.0 4 943.0 5 927.0 6 911.0 7 882.0 8 853.0 9 926.5 10 1000.0 11 1255.0 12 1510.0 13 2340.5 14 3171.0 15 4329.5 16 5488.0 17 6231.0 18 6974.0 19 7632.0 20 8290.0 21 9701.0 22 11112.0 23 14159.5 24 17207.0 25 22770.5 26 28334.0 27 35263.5 28 42193.0 29 50156.5 30 58120.0 31 66691.5 32 75263.0 33 85858.0 34 96453.0 35 107254.5 36 118056.0 37 127838.0 38 137620.0 39 148195.0 40 158770.0 41 169086.5 42 179403.0 43 187775.0 44 196147.0 45 205938.0 46 215729.0 47 223780.0 48 231831.0 49 235659.5 50 239488.0 51 240240.5 52 240993.0 53 236915.0 54 232837.0 55 225177.5 56 217518.0 57 205260.0 58 193002.0 59 177875.0 60 162748.0 61 144153.5 62 125559.0 63 108355.0 64 91151.0 65 76783.0 66 62415.0 67 51611.0 68 40807.0 69 33381.0 70 25955.0 71 20468.0 72 14981.0 73 12347.0 74 9713.0 75 7514.5 76 5316.0 77 4165.0 78 3014.0 79 2402.0 80 1790.0 81 1384.5 82 979.0 83 763.0 84 547.0 85 434.5 86 322.0 87 266.0 88 210.0 89 149.0 90 88.0 91 73.5 92 59.0 93 46.0 94 33.0 95 25.5 96 18.0 97 16.5 98 15.0 99 27.5 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08438896028394835 2 0.09506762132060462 3 0.10170736185055995 4 0.1031454623340065 5 0.09460865308120678 6 0.05709564898109051 7 0.053699284009546544 8 0.0596964690043449 9 0.06046141607000796 10 0.04562144299614467 11 0.04898721008506212 12 0.057462823572608776 13 0.05109846398629215 14 0.05253656446973869 15 0.052322379291353034 16 0.05510678661036656 17 0.055229178140872644 18 0.05519858025824613 19 0.06385778104155193 20 0.06860045284866287 21 0.07842237317177651 22 0.08135976990392264 23 0.05880913040817575 24 0.07658650021418519 25 0.08325683862676703 26 0.08105379107765742 27 0.07609693409216083 28 0.09439446790282112 29 0.08313444709626094 30 0.08077841013401874 31 0.05553515696713787 32 0.053607490361666975 33 0.0547396120188483 34 0.04932378679395386 35 0.05593292944128266 36 0.05525977602349918 37 0.05773820451624748 38 0.04889541643718255 39 0.05425004589682394 40 0.05535156967137875 41 0.042867633559757666 42 0.039195887644574996 43 0.03953246435346674 44 0.03830854904840585 45 0.02952695673459397 46 0.026528364237194783 47 0.031026252983293555 48 0.02591640658466434 49 0.026008200232543906 50 0.02689553882871305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3268200.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.04533928984418 #Duplication Level Percentage of deduplicated Percentage of total 1 77.08570410857534 46.28637257595942 2 12.553364060450559 15.075420084773796 3 4.433576604868461 7.986468345205264 4 2.0660104338637932 4.9621718991083865 5 1.1332998655084572 3.402468747079505 6 0.645999071676212 2.3273540063833513 7 0.4278356016208279 1.7982673701718053 8 0.30547645600221823 1.4673949956569883 9 0.21150038779462768 1.1429651290555833 >10 1.0590114644682627 11.105600334918684 >50 0.059484325128253475 2.4328899650476843 >100 0.017967715684076884 1.6635963251365464 >500 6.159234877624911E-4 0.23194714794141738 >1k 1.5398087125181786E-4 0.1170830735616472 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.059788262652224E-5 0.0 0.0 0.0 0.0 8 3.059788262652224E-5 0.0 0.0 0.0 0.0 9 3.059788262652224E-5 0.0 0.0 3.059788262652224E-5 0.0 10 3.059788262652224E-5 0.0 0.0 3.059788262652224E-5 0.0 11 3.059788262652224E-5 0.0 0.0 3.059788262652224E-5 0.0 12 3.059788262652224E-5 0.0 0.0 3.059788262652224E-5 0.0 13 3.059788262652224E-5 0.0 0.0 3.059788262652224E-5 0.0 14 9.179364787956673E-5 0.0 0.0 3.059788262652224E-5 3.059788262652224E-5 15 9.179364787956673E-5 0.0 0.0 3.059788262652224E-5 3.059788262652224E-5 16 9.179364787956673E-5 0.0 0.0 6.119576525304448E-5 3.059788262652224E-5 17 9.179364787956673E-5 0.0 0.0 6.119576525304448E-5 3.059788262652224E-5 18 1.2239153050608897E-4 0.0 0.0 6.119576525304448E-5 3.059788262652224E-5 19 2.4478306101217794E-4 0.0 0.0 6.119576525304448E-5 3.059788262652224E-5 20 2.4478306101217794E-4 0.0 0.0 6.119576525304448E-5 3.059788262652224E-5 21 2.4478306101217794E-4 3.059788262652224E-5 0.0 9.179364787956673E-5 3.059788262652224E-5 22 2.753809436387002E-4 3.059788262652224E-5 0.0 1.2239153050608897E-4 3.059788262652224E-5 23 3.0597882626522246E-4 3.059788262652224E-5 0.0 1.8358729575913347E-4 3.059788262652224E-5 24 3.977724741447892E-4 3.059788262652224E-5 0.0 3.365767088917447E-4 3.059788262652224E-5 25 3.977724741447892E-4 3.059788262652224E-5 0.0 3.977724741447892E-4 3.059788262652224E-5 26 3.977724741447892E-4 3.059788262652224E-5 0.0 6.119576525304449E-4 3.059788262652224E-5 27 3.977724741447892E-4 3.059788262652224E-5 0.0 7.649470656630561E-4 3.059788262652224E-5 28 5.201640046508781E-4 3.059788262652224E-5 0.0 0.001866470840217857 3.059788262652224E-5 29 5.813597699039227E-4 3.059788262652224E-5 0.0 0.0028150052016400464 3.059788262652224E-5 30 6.119576525304449E-4 3.059788262652224E-5 0.0 0.005109846398629215 3.059788262652224E-5 31 6.119576525304449E-4 3.059788262652224E-5 0.0 0.011137629276054098 3.059788262652224E-5 32 6.119576525304449E-4 3.059788262652224E-5 0.0 0.017685576158129858 3.059788262652224E-5 33 6.731534177834893E-4 3.059788262652224E-5 0.0 0.02561042775839912 3.059788262652224E-5 34 6.731534177834893E-4 3.059788262652224E-5 0.0 0.03717642739122453 3.059788262652224E-5 35 7.037513004100116E-4 3.059788262652224E-5 0.0 0.05140444281255737 3.059788262652224E-5 36 7.649470656630561E-4 3.059788262652224E-5 0.0 0.06780490790037329 3.059788262652224E-5 37 7.649470656630561E-4 3.059788262652224E-5 0.0 0.09093690716602411 3.059788262652224E-5 38 8.567407135426228E-4 3.059788262652224E-5 0.0 0.11777125022948412 3.059788262652224E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2230 0.0 23.092861 1 GTATCAA 4215 0.0 20.310413 1 CGTGCCG 270 0.0 18.737772 10 TATCAAC 5145 0.0 16.3825 2 TACCGAT 340 0.0 16.173414 7 GTCTAGG 740 0.0 15.761985 1 GTATACG 140 1.5826645E-6 15.7195 1 TCAACGC 5285 0.0 15.656798 4 ATCAACG 5345 0.0 15.646215 3 CAACGCA 5350 0.0 15.505573 5 TATAGCG 100 4.940188E-4 15.40275 5 AACGCAG 5575 0.0 14.952999 6 GTATTAC 330 0.0 14.671534 1 GTACATG 8685 0.0 14.367459 1 GGACCGT 295 0.0 14.166597 6 TACATGG 8710 0.0 13.871421 2 GCGTAAC 115 0.0014264747 13.389184 11 CGAATCG 280 5.820766E-11 13.35564 37 CTAGGAC 795 0.0 13.287631 3 TATACGA 100 0.008745491 13.20438 2 >>END_MODULE