FastQCFastQC Report
Mon 6 Feb 2017
SRR2077108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268200
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.024.8948881
GTATCAA48900.022.58611
CGCACGA2650.016.60010735
TACCGAT2750.016.0018527
TATCAAC68100.015.9915732
TATACTG6350.015.9386095
ATCAACG69650.015.5414093
TCAACGC68750.015.5213224
ATACCGT2152.1645974E-1015.3499116
CAACGCA71200.014.9874625
CGCGGTA1201.1822154E-414.66634631
AACGCAG75050.014.2768146
GTACCGA3100.014.1945416
TAAGACG1402.7572565E-514.1440624
CGTGCCG3600.014.05804410
CGACGGT950.006238048413.8963467
GCACCGT5100.013.8048866
GCGATAC2907.2759576E-1213.6575914
GCGCGTT1453.8477046E-513.65215736
CGTACAC3550.013.6345473
Produced by FastQC (version 0.11.5)