FastQCFastQC Report
Mon 6 Feb 2017
SRR2077107_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077107_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812095
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32750.019.2199251
GGTATCA18300.017.6792261
ATCAACG38200.015.8437713
TCAACGC38500.015.49082854
CAACGCA39400.015.1912095
TATCAAC40200.015.1650192
TCTATAC4650.015.1455933
AACGCAG40650.014.66550356
GTACATG61350.014.5649311
TACGACC950.006228913613.8993234
TACATGG62600.013.7113282
GTCCTAT5350.013.5755411
CGTGCGC2959.094947E-1213.42123210
CGTCGTA1000.00877505613.19754410
TTATACT7900.013.0929284
ACATGGG64550.013.05845453
GTATTAT5400.013.042271
TGGACCG2704.674803E-1013.0399485
GTGTACG1701.1818212E-512.9463711
CGATGCG1557.209246E-512.7684144
Produced by FastQC (version 0.11.5)