FastQCFastQC Report
Mon 6 Feb 2017
SRR2077107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812095
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37250.019.0795651
GGTATCA21650.016.5661621
ACCGTCG953.3442077E-416.2131218
GCGTATC1351.9571406E-514.6671924
TGCGTAT1503.2528205E-614.66718923
TATCAAC50250.014.0131912
TCAACGC50300.013.999514
ATCAACG50150.013.9972553
CAACGCA51150.013.8091545
GTACATG62200.013.58416751
AACGCAG52600.013.5114296
TACATGG62050.013.2278772
ACATGGG63450.013.0053713
TATACTG6800.012.9437095
CAATACT5500.012.8031894
TAGTCGG1557.205087E-512.769196540
TCGCGTC1902.6502748E-612.73933417
GACCGTG4150.012.7249297
GTCTACG1404.3569948E-412.57344151
ATACCGT1953.5469948E-612.4120246
Produced by FastQC (version 0.11.5)