FastQCFastQC Report
Mon 6 Feb 2017
SRR2077106_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077106_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4131735
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41350.022.0870421
GGTATCA20800.019.5737191
TCAACGC53350.016.500254
ATCAACG53500.016.4953213
TATCAAC54950.016.3402022
CAACGCA54900.015.9519045
GTACATG105400.015.8268161
AACGCAG57000.015.2823696
TACATGG107500.015.1491472
ACATGGG113000.014.295123
CGCGTAA1100.0010202213.99729510
TAGATCG950.00623567313.8972695
CGAATCG2401.1314114E-913.74867637
CTTACAC10250.013.7412533
GTATAGG7350.013.4738011
GTCCTAA6300.013.274191
CATGGGG86350.012.9978884
TTATACT8650.012.9753484
GTATTAT5650.012.8537671
GTGTACG3950.012.8143241
Produced by FastQC (version 0.11.5)