##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077106_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4131735 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.55642242302568 31.0 30.0 34.0 25.0 34.0 2 29.66724342195228 31.0 30.0 34.0 25.0 34.0 3 29.750244630887508 31.0 30.0 34.0 25.0 34.0 4 33.18624088911801 37.0 35.0 37.0 27.0 37.0 5 33.147970283670176 37.0 35.0 37.0 26.0 37.0 6 33.280162691944184 37.0 35.0 37.0 27.0 37.0 7 33.15846272812753 37.0 35.0 37.0 26.0 37.0 8 33.099202877241645 37.0 35.0 37.0 26.0 37.0 9 34.54161048566764 39.0 35.0 39.0 27.0 39.0 10 34.47448614202024 39.0 35.0 39.0 27.0 39.0 11 34.52334309920651 39.0 35.0 39.0 27.0 39.0 12 34.42358863770305 39.0 35.0 39.0 26.0 39.0 13 34.368749447871174 39.0 35.0 39.0 26.0 39.0 14 35.3740222932981 40.0 36.0 41.0 25.0 41.0 15 35.41187394641718 40.0 36.0 41.0 25.0 41.0 16 35.381476062719415 40.0 36.0 41.0 25.0 41.0 17 35.22530994848411 39.0 35.0 41.0 25.0 41.0 18 35.20092697135707 39.0 35.0 41.0 25.0 41.0 19 35.31441682489317 40.0 35.0 41.0 25.0 41.0 20 35.298512610319875 40.0 35.0 41.0 25.0 41.0 21 35.247141213073924 40.0 35.0 41.0 25.0 41.0 22 35.17592560994352 39.0 35.0 41.0 25.0 41.0 23 35.1383806076624 39.0 35.0 41.0 24.0 41.0 24 35.14142242907641 39.0 35.0 41.0 24.0 41.0 25 35.04975101258914 39.0 35.0 41.0 24.0 41.0 26 34.99735583235614 39.0 35.0 41.0 24.0 41.0 27 34.94291962093406 39.0 35.0 41.0 24.0 41.0 28 34.83801550680283 39.0 34.0 41.0 23.0 41.0 29 34.825726964580255 39.0 35.0 41.0 23.0 41.0 30 34.676071916519334 39.0 34.0 41.0 22.0 41.0 31 34.678037192607945 39.0 34.0 41.0 22.0 41.0 32 34.552592796972704 39.0 34.0 41.0 21.0 41.0 33 34.51217732986264 39.0 34.0 41.0 21.0 41.0 34 34.441551793616966 39.0 34.0 41.0 19.0 41.0 35 34.38134585107709 39.0 34.0 41.0 19.0 41.0 36 34.29847630595864 39.0 34.0 41.0 18.0 41.0 37 34.211945829052446 39.0 34.0 41.0 18.0 41.0 38 34.1299187871439 39.0 34.0 41.0 17.0 41.0 39 34.02163594712633 39.0 33.0 41.0 16.0 41.0 40 33.95967553582212 39.0 33.0 41.0 15.0 41.0 41 33.85909188270787 38.0 33.0 41.0 15.0 41.0 42 33.72067739097498 38.0 33.0 40.0 14.0 41.0 43 33.64217743877572 38.0 33.0 40.0 11.0 41.0 44 33.50823782260963 38.0 33.0 40.0 9.0 41.0 45 33.37561339243683 38.0 33.0 40.0 8.0 41.0 46 33.244919628194936 38.0 33.0 40.0 2.0 41.0 47 33.09822362760438 38.0 33.0 40.0 2.0 41.0 48 32.951762637245615 38.0 33.0 40.0 2.0 41.0 49 32.786274289130354 38.0 33.0 40.0 2.0 41.0 50 32.58458347401273 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9598954562403073 1101 2 1.2462480875870305 1101 3 1.2582437599312186 1101 4 1.3799870940432655 1101 5 1.4393166425561645 1101 6 1.554277710402289 1101 7 1.6381087309881863 1101 8 1.7746441995388125 1101 9 1.9765808202522805 1101 10 2.0486833851817963 1101 11 1.9941403419575892 1101 12 2.0274973478313 1101 13 2.0867824939518727 1101 14 2.3783911504800983 1101 15 2.3431170586404306 1101 16 2.320496402922849 1101 17 2.37471859349084 1101 18 2.3740093860682023 1101 19 2.3780651716192622 1101 20 2.3473827627674737 1101 21 2.367962276397428 1101 22 2.4228567686199796 1101 23 2.4246179234204774 1101 24 2.4112416466765296 1101 25 2.3771852078795916 1101 26 2.3779508438179917 1101 27 2.3677324464046166 1101 28 2.438324564693133 1101 29 2.4592661752521465 1101 30 2.590531268147835 1101 31 2.60558558306802 1101 32 2.6129963321844727 1101 33 2.6468249251417078 1101 34 2.612091131807304 1101 35 2.561863419410976 1101 36 2.502075112230166 1101 37 2.618379735635294 1101 38 2.576872647742526 1101 39 2.6660242145362005 1101 40 2.6387477599531053 1101 41 2.558106879399567 1101 42 2.6466587518166236 1101 43 2.6469070329174613 1101 44 2.7638151171045493 1101 45 2.7294726100875053 1101 46 2.696702390070726 1101 47 2.7447509170131994 1101 48 2.5627624502439943 1101 49 2.740743320851159 1101 50 2.809441764122731 1102 1 0.3950756751046569 1102 2 0.5342730281686165 1102 3 0.5173779505210021 1102 4 0.7510789046609503 1102 5 0.6080364369516076 1102 6 0.7515248778250623 1102 7 0.9177489072431939 1102 8 0.8701117943235346 1102 9 1.0134801709729544 1102 10 0.9882340978141002 1102 11 0.906859008876161 1102 12 0.9418889795375094 1102 13 0.922745254769616 1102 14 1.2037978977893005 1102 15 1.0611526442350012 1102 16 1.1450880727749038 1102 17 1.2577261380550766 1102 18 1.3242034781479575 1102 19 1.242919640018087 1102 20 1.2548207191502954 1102 21 1.158179138823506 1102 22 1.0941524781581435 1102 23 1.0671088970150038 1102 24 1.0617986763562755 1102 25 1.205232986402038 1102 26 1.230229066987441 1102 27 1.107752197528292 1102 28 1.2088057938592272 1102 29 1.054938694141157 1102 30 1.2346325125970594 1102 31 1.1622312131882353 1102 32 1.2572727028129833 1102 33 1.3204186799472595 1102 34 1.34170177488258 1102 35 1.2708889912128782 1102 36 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-2.21861885423678 2216 47 -2.110681317807856 2216 48 -2.1679067884297005 2216 49 -2.215004690632103 2216 50 -2.245276555351147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 164869.0 3 8490.0 4 15615.0 5 12561.0 6 10794.0 7 9281.0 8 8478.0 9 7814.0 10 7615.0 11 7670.0 12 7784.0 13 8215.0 14 8820.0 15 9352.0 16 10102.0 17 10809.0 18 12006.0 19 13775.0 20 16518.0 21 20626.0 22 24926.0 23 28962.0 24 31703.0 25 35549.0 26 39772.0 27 45482.0 28 51899.0 29 60597.0 30 71869.0 31 86556.0 32 106221.0 33 132963.0 34 171298.0 35 224926.0 36 300620.0 37 419507.0 38 670422.0 39 1257269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.71444628302573 16.614864215079614 12.19134423062156 25.4793452712731 2 16.63088439151478 18.59488795569103 38.527553320919765 26.246674331874427 3 20.29391099567771 22.450695830829765 27.609704804915395 29.645688368577133 4 13.500936309851328 14.819886644604207 35.16420735416935 36.51496969137512 5 15.28905317659782 36.355535915971004 33.33372095671264 15.021689950718534 6 35.41875438026328 34.07153433085076 16.34126991430546 14.168441374580501 7 30.940350759936276 30.298552533472044 19.84121045426664 18.919886252325043 8 27.584851559718786 34.513146391492924 18.760337783676047 19.141664265112233 9 27.033203888903017 15.572055986835172 17.984759704396872 39.40998041986494 10 15.198337567947334 28.313598571184013 32.71239920646086 23.775664654407798 11 37.24045330169262 21.843499527810735 21.805215875245175 19.110831295251472 12 24.230966692013737 23.60544300057636 29.05689529077286 23.10669501663704 13 29.190516987950815 20.484364731083645 24.93065870716931 25.39445957379623 14 22.838609596557895 19.957336900457285 25.59074032997188 31.613313173012937 15 24.811420678512636 27.952794993754786 22.224051848597988 25.01173247913459 16 25.082577942922903 26.80768202503395 23.885197770540838 24.224542261502307 17 23.94559826339102 26.87801479478872 25.208001013215636 23.968385928604622 18 23.96741687668624 26.584857715824928 25.678546666966955 23.769178740521877 19 24.888744867732633 26.10641791048391 25.578908133958084 23.425929087825377 20 24.928562467090778 25.67161453426216 25.600479754077593 23.799343244569464 21 25.06859199829861 25.458040978132672 25.56055049938269 23.912816524186024 22 24.914213792358378 25.197454827499836 25.4115630554666 24.47676832467518 23 24.629440286886815 25.331787952129346 25.4715454990611 24.56722626192274 24 24.419679990156226 25.41343122850005 25.480792288139657 24.686096493204065 25 24.60065840311809 25.34614611316879 25.55151556258584 24.501679921127277 26 24.45603543539208 25.612782204402684 25.623416116359703 24.30776624384553 27 24.607771920701055 25.669118468103285 25.380807546707313 24.342302064488344 28 24.517440088534123 25.600689395528814 25.437062132329245 24.444808383607814 29 24.56500727914869 25.764044154516586 25.403841355738415 24.267107210596308 30 24.668597892639887 25.791870877382006 25.329209917997588 24.210321311980522 31 24.69876892169009 25.30476671119304 25.338548956891433 24.657915410225435 32 24.462007951525624 25.805504850198158 25.360344369154593 24.372142829121625 33 24.589753444017507 25.463922543842248 25.30750783344921 24.638816178691034 34 24.779912257909537 25.46094624592243 25.403168294869992 24.355973201298042 35 24.706713140835088 25.591774333733962 25.369606949717376 24.331905575713574 36 24.694328331172784 25.651258830387064 25.258170234581772 24.396242603858383 37 24.7003430670475 25.362235307280645 25.44978206548352 24.487639560188335 38 24.709874966250453 25.452885631712828 25.46099763056989 24.37624177146683 39 24.75373690041592 25.711739527900374 25.243175502642618 24.29134806904109 40 24.82987694957383 25.271930495834226 25.516689737208925 24.381502817383016 41 24.52358249113898 25.56763068036261 25.45632010181395 24.452466726684467 42 24.739207535694018 25.53591870810033 25.40887590239858 24.315997853807072 43 24.543775516973707 25.258456587186632 25.516198904915967 24.681568990923694 44 24.57495080709772 25.56210169608072 25.35181974393784 24.51112775288372 45 24.682056001140776 25.48994400951163 25.246972356769348 24.581027632578248 46 24.683675922426794 25.2647220669468 25.272081678126924 24.779520332499487 47 24.547142238737024 25.518952478624534 25.383010413428508 24.550894869209934 48 24.423310821933992 25.652948399379465 25.43760688395622 24.48613389473032 49 24.66586485853122 25.388775267124124 25.326678713156042 24.61868116118861 50 24.71083131548329 25.438153077711554 25.388548101816816 24.46246750498834 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2054.0 1 1507.5 2 961.0 3 927.0 4 893.0 5 824.5 6 756.0 7 743.5 8 731.0 9 824.0 10 917.0 11 1068.0 12 1219.0 13 1743.0 14 2267.0 15 2954.0 16 3641.0 17 3990.0 18 4339.0 19 4724.5 20 5110.0 21 6099.0 22 7088.0 23 9413.5 24 11739.0 25 16532.5 26 21326.0 27 27930.5 28 34535.0 29 44119.0 30 53703.0 31 65847.0 32 77991.0 33 92101.5 34 106212.0 35 121217.5 36 136223.0 37 151432.0 38 166641.0 39 181721.5 40 196802.0 41 213302.5 42 229803.0 43 243723.0 44 257643.0 45 272398.5 46 287154.0 47 298525.5 48 309897.0 49 315146.5 50 320396.0 51 322519.0 52 324642.0 53 319206.0 54 313770.0 55 302482.0 56 291194.0 57 273449.0 58 255704.0 59 234250.5 60 212797.0 61 187926.0 62 163055.0 63 140740.0 64 118425.0 65 99034.0 66 79643.0 67 65445.0 68 51247.0 69 41996.0 70 32745.0 71 25976.5 72 19208.0 73 15995.5 74 12783.0 75 10043.5 76 7304.0 77 5659.0 78 4014.0 79 3143.5 80 2273.0 81 1766.0 82 1259.0 83 992.5 84 726.0 85 543.0 86 360.0 87 291.5 88 223.0 89 166.0 90 109.0 91 82.5 92 56.0 93 51.5 94 47.0 95 41.5 96 36.0 97 30.5 98 25.0 99 37.0 100 49.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08596872742322535 2 0.09664220962864269 3 0.10395148769221647 4 0.10557308249440005 5 0.09722307940852934 6 0.05849842741608549 7 0.05431132441940251 8 0.05966016697585881 9 0.06171741411295739 10 0.04554987190611208 11 0.04925291675288953 12 0.057965963451189394 13 0.05230248309729448 14 0.05254451217224725 15 0.0525929179872378 16 0.055739295961623865 17 0.055860310499100264 18 0.057070455873864126 19 0.06563828512719233 20 0.07147118583355419 21 0.08078930521923598 22 0.08347582795121178 23 0.05907929719597215 24 0.07834481156221297 25 0.08628336522066396 26 0.08308858143128733 27 0.07759452142985937 28 0.09828800733832155 29 0.08483119077094732 30 0.08330640759874483 31 0.056804223891416074 32 0.0535610342870489 33 0.0563443686490058 34 0.05160059877993143 35 0.05847422450859022 36 0.0577481372837319 37 0.06082190653563213 38 0.04961596036531868 39 0.05590871631409081 40 0.057385093671302734 41 0.04475117595876792 42 0.03947494212479745 43 0.03971697119975023 44 0.03790175313760442 45 0.02933392388427622 46 0.026187545909890152 47 0.031439576836365356 48 0.026308560447366545 49 0.025921313927442104 50 0.026647401152300427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4131735.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.660504690220684 #Duplication Level Percentage of deduplicated Percentage of total 1 75.32960344200905 43.43542952580426 2 13.183275463473143 15.203086333881288 3 4.811344155861766 8.322735967959996 4 2.235202599943616 5.1553163999056935 5 1.240099990063806 3.5752395646708344 6 0.7539283928417567 2.6083134978925586 7 0.5025919923058708 2.028579555073401 8 0.3602725012469013 1.6618795398323591 9 0.252064627362501 1.3080756265446793 >10 1.2524144767512564 12.48133919363364 >50 0.06018804926422457 2.356964057084312 >100 0.018382467937957104 1.6106622874579721 >500 5.897182165372271E-4 0.2257100353712611 >1k 4.212272157673385E-5 0.026668414887740542 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4202907495277407E-5 2 2.4202907495277407E-5 0.0 0.0 0.0 2.4202907495277407E-5 3 2.4202907495277407E-5 0.0 0.0 0.0 2.4202907495277407E-5 4 2.4202907495277407E-5 0.0 0.0 0.0 2.4202907495277407E-5 5 2.4202907495277407E-5 0.0 0.0 0.0 2.4202907495277407E-5 6 2.4202907495277407E-5 0.0 0.0 0.0 1.6942035246694185E-4 7 2.4202907495277407E-5 0.0 0.0 0.0 1.6942035246694185E-4 8 2.4202907495277407E-5 0.0 0.0 0.0 1.6942035246694185E-4 9 2.4202907495277407E-5 0.0 0.0 0.0 1.9362325996221926E-4 10 4.8405814990554815E-5 0.0 0.0 2.4202907495277407E-5 1.9362325996221926E-4 11 4.8405814990554815E-5 0.0 0.0 2.4202907495277407E-5 1.9362325996221926E-4 12 4.8405814990554815E-5 0.0 0.0 2.4202907495277407E-5 3.388407049338837E-4 13 4.8405814990554815E-5 0.0 0.0 2.4202907495277407E-5 3.872465199244385E-4 14 7.260872248583222E-5 0.0 0.0 2.4202907495277407E-5 3.872465199244385E-4 15 7.260872248583222E-5 0.0 0.0 2.4202907495277407E-5 4.8405814990554815E-4 16 7.260872248583222E-5 0.0 0.0 2.4202907495277407E-5 5.082610574008256E-4 17 9.681162998110963E-5 0.0 0.0 2.4202907495277407E-5 5.082610574008256E-4 18 9.681162998110963E-5 0.0 0.0 2.4202907495277407E-5 5.32463964896103E-4 19 1.2101453747638704E-4 0.0 2.4202907495277407E-5 2.4202907495277407E-5 5.32463964896103E-4 20 1.2101453747638704E-4 0.0 2.4202907495277407E-5 7.260872248583222E-5 5.566668723913804E-4 21 1.2101453747638704E-4 0.0 2.4202907495277407E-5 1.2101453747638704E-4 5.566668723913804E-4 22 1.4521744497166444E-4 0.0 2.4202907495277407E-5 1.6942035246694185E-4 5.808697798866578E-4 23 1.4521744497166444E-4 0.0 2.4202907495277407E-5 1.9362325996221926E-4 7.018843173630449E-4 24 2.4202907495277407E-4 0.0 2.4202907495277407E-5 3.872465199244385E-4 7.502901323535997E-4 25 2.4202907495277407E-4 0.0 2.4202907495277407E-5 4.8405814990554815E-4 7.502901323535997E-4 26 2.4202907495277407E-4 0.0 2.4202907495277407E-5 6.5347850237249E-4 8.228988548394318E-4 27 2.4202907495277407E-4 0.0 2.4202907495277407E-5 9.439133923158189E-4 8.228988548394318E-4 28 3.146377974386063E-4 0.0 2.4202907495277407E-5 0.0018878267846316379 8.228988548394318E-4 29 3.388407049338837E-4 0.0 2.4202907495277407E-5 0.0029285518069285662 8.228988548394318E-4 30 3.630436124291611E-4 0.0 2.4202907495277407E-5 0.004985798944027146 8.228988548394318E-4 31 3.630436124291611E-4 0.0 2.4202907495277407E-5 0.010794496742893725 8.228988548394318E-4 32 3.630436124291611E-4 0.0 2.4202907495277407E-5 0.019168702736259705 8.228988548394318E-4 33 3.630436124291611E-4 0.0 2.4202907495277407E-5 0.028438416306950955 8.228988548394318E-4 34 3.630436124291611E-4 0.0 2.4202907495277407E-5 0.04041885551711327 8.713046698299867E-4 35 3.872465199244385E-4 0.0 4.8405814990554815E-5 0.05462596221684111 8.713046698299867E-4 36 4.114494274197159E-4 0.0 4.8405814990554815E-5 0.07381886786059609 8.713046698299867E-4 37 4.114494274197159E-4 0.0 4.8405814990554815E-5 0.09724728231602463 8.713046698299867E-4 38 4.3565233491499333E-4 0.0 4.8405814990554815E-5 0.1239430892833156 8.955075773252641E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4135 0.0 22.087042 1 GGTATCA 2080 0.0 19.573719 1 TCAACGC 5335 0.0 16.50025 4 ATCAACG 5350 0.0 16.495321 3 TATCAAC 5495 0.0 16.340202 2 CAACGCA 5490 0.0 15.951904 5 GTACATG 10540 0.0 15.826816 1 AACGCAG 5700 0.0 15.282369 6 TACATGG 10750 0.0 15.149147 2 ACATGGG 11300 0.0 14.29512 3 CGCGTAA 110 0.00102022 13.997295 10 TAGATCG 95 0.006235673 13.897269 5 CGAATCG 240 1.1314114E-9 13.748676 37 CTTACAC 1025 0.0 13.741253 3 GTATAGG 735 0.0 13.473801 1 GTCCTAA 630 0.0 13.27419 1 CATGGGG 8635 0.0 12.997888 4 TTATACT 865 0.0 12.975348 4 GTATTAT 565 0.0 12.853767 1 GTGTACG 395 0.0 12.814324 1 >>END_MODULE