FastQCFastQC Report
Mon 6 Feb 2017
SRR2077106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4131735
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49050.021.4414251
GGTATCA26350.020.290391
CGAAACG750.001292950517.59722933
CGAATCG2250.017.59637837
CGCGTAA800.001987032816.50299610
TACCGAT3350.015.7632937
ATCAACG66250.015.6764293
TCAACGC66700.015.6366444
TATCAAC67250.015.5089482
CAACGCA67800.015.3184175
AACGCAG71300.014.5962616
GTACATG97700.014.525461
TACATGG99100.014.1436692
GTACCGA4050.013.5817276
CGTGCCG3700.013.08345610
TGCGATA2207.37964E-813.00251913
CTAGTAC3450.012.75563053
ACATGGG106350.012.7448363
GGACCGT3800.012.7382296
GTACTAA3800.012.7380751
Produced by FastQC (version 0.11.5)