FastQCFastQC Report
Mon 6 Feb 2017
SRR2077105_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077105_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889740
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.020.8058971
GTATCAA41400.020.7305131
GCGATAC1852.1827873E-1117.83856814
TATCAAC50950.016.5421852
TCAACGC51950.016.2658374
CGTGCCG2301.8189894E-1216.25843810
ATCAACG52300.016.2414153
CAACGCA52350.016.1398745
GGACCGT2051.05501385E-1016.095996
CGTTACG1958.913048E-1015.79825524
AACGCAG53400.015.7362926
TGCGATA2152.1645974E-1015.34733913
GTACATG95200.015.279541
TACATGG96550.014.495832
CGATACT2501.382432E-1014.08106515
GCACCGT3750.014.0786266
ACATGGG98650.013.9864883
GTGCCGA3400.013.58714511
AGCACCG5600.013.3590775
GTATAGT3001.2732926E-1113.2037421
Produced by FastQC (version 0.11.5)