##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077105_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2889740
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.508173399683017	31.0	30.0	34.0	25.0	34.0
2	29.50795884750877	31.0	30.0	34.0	24.0	34.0
3	29.61345034501374	31.0	30.0	34.0	25.0	34.0
4	33.04346065736018	37.0	35.0	37.0	26.0	37.0
5	33.00819796936749	37.0	35.0	37.0	26.0	37.0
6	33.079245537660825	37.0	35.0	37.0	26.0	37.0
7	32.90202267332009	37.0	35.0	37.0	25.0	37.0
8	32.82855931675514	37.0	35.0	37.0	25.0	37.0
9	34.235467550713906	39.0	35.0	39.0	25.0	39.0
10	34.16745485753044	39.0	35.0	39.0	25.0	39.0
11	34.22549710354565	39.0	35.0	39.0	25.0	39.0
12	34.12717822364642	39.0	35.0	39.0	25.0	39.0
13	34.074365513852456	39.0	35.0	39.0	25.0	39.0
14	35.04907085066477	39.0	35.0	41.0	23.0	41.0
15	35.09754856838332	39.0	35.0	41.0	24.0	41.0
16	35.06801961422135	39.0	35.0	41.0	24.0	41.0
17	34.90883332064477	39.0	35.0	41.0	22.0	41.0
18	34.88259116737146	39.0	34.0	41.0	22.0	41.0
19	35.0013651747216	39.0	35.0	41.0	23.0	41.0
20	34.98086679078395	39.0	35.0	41.0	23.0	41.0
21	34.93292303113775	39.0	35.0	41.0	23.0	41.0
22	34.85657325572543	39.0	34.0	41.0	22.0	41.0
23	34.81342093060275	39.0	34.0	41.0	22.0	41.0
24	34.81435665492397	39.0	34.0	41.0	22.0	41.0
25	34.71859786693613	39.0	34.0	41.0	21.0	41.0
26	34.66844041332438	39.0	34.0	41.0	20.0	41.0
27	34.6220099386104	39.0	34.0	41.0	19.0	41.0
28	34.51069300352281	39.0	34.0	41.0	18.0	41.0
29	34.49591174292497	39.0	34.0	41.0	19.0	41.0
30	34.34830815229052	39.0	34.0	41.0	18.0	41.0
31	34.345258050897314	39.0	34.0	41.0	18.0	41.0
32	34.212473786569035	39.0	34.0	41.0	16.0	41.0
33	34.18119380982372	39.0	34.0	41.0	16.0	41.0
34	34.09767314706514	39.0	34.0	41.0	16.0	41.0
35	34.034329039982836	39.0	34.0	41.0	16.0	41.0
36	33.94898987452159	39.0	33.0	41.0	15.0	41.0
37	33.859362087938706	39.0	33.0	41.0	15.0	41.0
38	33.7713181808744	38.0	33.0	41.0	14.0	41.0
39	33.66314062856866	38.0	33.0	41.0	9.0	41.0
40	33.593520870389725	38.0	33.0	40.0	9.0	41.0
41	33.48938970287984	38.0	33.0	40.0	9.0	41.0
42	33.34660211645338	38.0	33.0	40.0	8.0	41.0
43	33.26064524836144	38.0	33.0	40.0	7.0	41.0
44	33.11873490348613	38.0	33.0	40.0	2.0	41.0
45	32.9788987936631	38.0	33.0	40.0	2.0	41.0
46	32.84417525452117	38.0	32.0	40.0	2.0	41.0
47	32.69035587976773	38.0	32.0	40.0	2.0	41.0
48	32.53509139230519	38.0	32.0	40.0	2.0	41.0
49	32.36348737256639	38.0	32.0	40.0	2.0	41.0
50	32.15236664890267	38.0	32.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.0381445887780814
1101	2	1.1945574966665014
1101	3	1.2119652544098116
1101	4	1.2395369766897275
1101	5	1.3553349020862129
1101	6	1.3678710041571165
1101	7	1.3660850738851948
1101	8	1.465973512941929
1101	9	1.582866222264201
1101	10	1.6277503207821553
1101	11	1.6219737039266846
1101	12	1.629446097008568
1101	13	1.6305930210920394
1101	14	1.9518939358638647
1101	15	2.0339135532338943
1101	16	1.8406576323985533
1101	17	2.082177779857645
1101	18	2.1060515953831924
1101	19	2.060487010714901
1101	20	2.105004495860598
1101	21	2.020599490767026
1101	22	2.0581336691678302
1101	23	2.1410620318568903
1101	24	2.0478028920897486
1101	25	2.014810465027267
1101	26	2.1738674841104313
1101	27	2.1365460685529314
1101	28	2.152247035774984
1101	29	2.084330836410409
1101	30	2.289817677139574
1101	31	2.3597146510210436
1101	32	2.358108763938091
1101	33	2.3648821362434447
1101	34	2.3191536834843944
1101	35	2.3796789276618213
1101	36	2.51339484558369
1101	37	2.5719470371521354
1101	38	2.4186554449732256
1101	39	2.386981858589202
1101	40	2.5201778451852377
1101	41	2.5299490696623366
1101	42	2.5919860405849278
1101	43	2.6028138271346464
1101	44	2.474866152162434
1101	45	2.6150219506046355
1101	46	2.635500088347804
1101	47	2.5300301014767683
1101	48	2.351484769775844
1101	49	2.424197605173397
1101	50	2.5323770604882654
1102	1	0.6572584320973611
1102	2	0.7028220326832262
1102	3	0.712877630840385
1102	4	0.8164556246010051
1102	5	0.8175362390572403
1102	6	0.7516581635661055
1102	7	0.8375489508649636
1102	8	0.7598443511471729
1102	9	0.8050623625280551
1102	10	0.8890723512773775
1102	11	0.7990677407051976
1102	12	0.8641571482788066
1102	13	0.8922546091585133
1102	14	1.120522654757437
1102	15	0.9733128611269848
1102	16	1.041817442954951
1102	17	1.2214849643053185
1102	18	1.201124762658111
1102	19	1.1651194617890823
1102	20	1.0661960181414756
1102	21	1.0575418781815458
1102	22	1.042725137119497
1102	23	1.016809058513637
1102	24	0.8758007425426868
1102	25	1.192635288752598
1102	26	1.1643332980446885
1102	27	1.0100018197723912
1102	28	1.2327354671961643
1102	29	1.0852934083080328
1102	30	1.0479286740806018
1102	31	1.2026810850169554
1102	32	1.2671796753696682
1102	33	1.2112042848456497
1102	34	1.1733919525084744
1102	35	1.2741459108551965
1102	36	1.1818759307506355
1102	37	1.2003027517584286
1102	38	1.1269813966202236
1102	39	1.274852566876767
1102	40	1.2574577232988347
1102	41	1.3302227825915054
1102	42	1.2120609794645603
1102	43	1.1929117378221505
1102	44	0.9699647124383333
1102	45	1.010396881216785
1102	46	0.8692072302769631
1102	47	0.9016699578586724
1102	48	0.8923335455969834
1102	49	0.8883447546614036
1102	50	0.9341017176827506
1103	1	0.3835236164008293
1103	2	0.47271868292567376
1103	3	0.4180389781930067
1103	4	0.4276451792307583
1103	5	0.5257643062494282
1103	6	0.5003252985321467
1103	7	0.3757529687925967
1103	8	0.46626990436797655
1103	9	0.6570380932842568
1103	10	0.5767009817783801
1103	11	0.4987668887242407
1103	12	0.5579214115035427
1103	13	0.5686855538951363
1103	14	0.5313998523568273
1103	15	0.5679838976880731
1103	16	0.5264593828749824
1103	17	0.7608798054546639
1103	18	0.5078466805532216
1103	19	0.5532665955135201
1103	20	0.53182915349349
1103	21	0.3587887758669055
1103	22	0.2959096065848925
1103	23	0.5411317788606382
1103	24	0.40860194203686717
1103	25	0.41815618745552996
1103	26	0.5713073823304811
1103	27	0.4707815787129235
1103	28	0.4534514051647207
1103	29	0.5673435068938275
1103	30	0.6535963110851597
1103	31	0.5883544644244694
1103	32	0.700560884553731
1103	33	0.6408355564471364
1103	34	0.7649934014024353
1103	35	0.6531321911681331
1103	36	0.6672069927645339
1103	37	0.5971736482345733
1103	38	0.7100792307018935
1103	39	0.7146047393404942
1103	40	0.7071108173811211
1103	41	0.6442841460039688
1103	42	0.7630325657038526
1103	43	0.661844730525047
1103	44	0.495416513366564
1103	45	0.4380419291871007
1103	46	0.4211847492079954
1103	47	0.5333314152009194
1103	48	0.5692321149221584
1103	49	0.4873409812751106
1103	50	0.5613200666508078
1104	1	0.6607010900611208
1104	2	0.6789830208417484
1104	3	0.6157549992006608
1104	4	0.6734976301939923
1104	5	0.66346432633636
1104	6	0.5539996528363673
1104	7	0.6131129815682357
1104	8	0.6726294811915636
1104	9	0.7665752870921523
1104	10	0.5422908164783422
1104	11	0.6745610993554507
1104	12	0.7387904393304581
1104	13	0.7548731085668265
1104	14	0.870045683515059
1104	15	0.8294997913118536
1104	16	0.8495260796181228
1104	17	0.8869932257294053
1104	18	0.7979880174447516
1104	19	0.9602494063367004
1104	20	0.8428934931349445
1104	21	0.8576967880225865
1104	22	0.9371141537795253
1104	23	0.8487121292751141
1104	24	0.7912169740205712
1104	25	0.9449464558920013
1104	26	0.8971323801762097
1104	27	0.980058769813148
1104	28	0.892578352109247
1104	29	0.9506357987772063
1104	30	1.0130315262275502
1104	31	0.946345018567861
1104	32	0.9346571388854414
1104	33	0.9663570936256534
1104	34	0.9534306137679494
1104	35	0.9407343303624245
1104	36	0.9638827088429878
1104	37	0.9975499276979818
1104	38	0.9516137760544865
1104	39	1.082050806326528
1104	40	1.0906812284416603
1104	41	1.1428722769911417
1104	42	1.1655093458896104
1104	43	1.1342634840903756
1104	44	1.1881453585406447
1104	45	1.146162322930138
1104	46	1.204993899652436
1104	47	1.0521926402016888
1104	48	1.158016294930853
1104	49	1.301890976701877
1104	50	1.3807761157331058
1105	1	0.7041725335986015
1105	2	0.6492572804902252
1105	3	0.6589458458890682
1105	4	0.7341127022160236
1105	5	0.6811278513748249
1105	6	0.7681980584869379
1105	7	0.7829544322297082
1105	8	0.8313340335911121
1105	9	0.762209256053481
1105	10	0.8356058808748514
1105	11	0.7718180181186511
1105	12	0.7352989196011421
1105	13	0.8377959097549166
1105	14	1.0038880499849867
1105	15	0.9563731015548314
1105	16	0.8846478558539772
1105	17	1.0108579970653437
1105	18	0.8979929208979982
1105	19	0.7765366830687697
1105	20	0.9293190006376904
1105	21	1.0166270297814748
1105	22	0.9594678411670401
1105	23	0.9563999795434128
1105	24	0.9607932093878233
1105	25	0.9063469567154243
1105	26	0.9772409161560915
1105	27	0.9878261753475641
1105	28	0.9078435664712572
1105	29	0.899405432865862
1105	30	0.906716456424185
1105	31	0.7062740918879129
1105	32	1.0432098054224639
1105	33	0.974611674951845
1105	34	0.9035315447342214
1105	35	0.9274070054683747
1105	36	0.9789165473314441
1105	37	0.9903535973454325
1105	38	0.9829862749903597
1105	39	1.02350432037791
1105	40	1.0777002215855873
1105	41	1.0082709939270913
1105	42	1.014980322946478
1105	43	1.0686724324637282
1105	44	1.0292642445357316
1105	45	1.0637813253589314
1105	46	1.0591413354913044
1105	47	0.9334410705839318
1105	48	1.0862131460189417
1105	49	1.0243097513717956
1105	50	1.1331835155101544
1106	1	0.4688591017258439
1106	2	0.4765655134636013
1106	3	0.4161581665431697
1106	4	0.4543298760980079
1106	5	0.4109587488763111
1106	6	0.3564012580223519
1106	7	0.46500726005304216
1106	8	0.5341563722662244
1106	9	0.5799992629208148
1106	10	0.5830865808826857
1106	11	0.5341108802325962
1106	12	0.5552131008897163
1106	13	0.5870923530256178
1106	14	0.706852897497761
1106	15	0.6567595162039268
1106	16	0.608544633734688
1106	17	0.7587375558963316
1106	18	0.49362492362649846
1106	19	0.5321450879561027
1106	20	0.5148403423961909
1106	21	0.6500230448804061
1106	22	0.6188589984291397
1106	23	0.5966960587067973
1106	24	0.5299286158283749
1106	25	0.5063839205213156
1106	26	0.5351870743630229
1106	27	0.7241467557069683
1106	28	0.7123501088805426
1106	29	0.6251388786576584
1106	30	0.6575494325636342
1106	31	0.5970442940826999
1106	32	0.7479726056381608
1106	33	0.7272515451637247
1106	34	0.7691829511563384
1106	35	0.7112288417675714
1106	36	0.6563801481223379
1106	37	0.6580620718279633
1106	38	0.7727395577173866
1106	39	0.7162024477601605
1106	40	0.8129531839903592
1106	41	0.8063682336249229
1106	42	0.7659168161782333
1106	43	0.7438318331154292
1106	44	0.7358567992324865
1106	45	0.7560539541676548
1106	46	0.700577393176971
1106	47	0.8016773624044262
1106	48	0.9323055370978537
1106	49	0.9413783648603555
1106	50	0.9914595825980825
1107	1	0.2035661785376277
1107	2	0.12492683308880004
1107	3	0.2187159648175232
1107	4	0.1304919653748513
1107	5	0.09400942638495025
1107	6	0.12919322544150447
1107	7	0.2022923722614749
1107	8	0.2812909193690416
1107	9	0.25797211679002174
1107	10	0.1446283565757014
1107	11	0.1950068078551226
1107	12	0.051822375626962014
1107	13	0.2696662219559087
1107	14	0.11215685495402283
1107	15	0.2756233986637895
1107	16	0.08493899206452937
1107	17	0.11703163974303266
1107	18	0.21893776343959814
1107	19	0.07346887962363269
1107	20	0.2089935081093799
1107	21	0.2526149532364599
1107	22	-0.021041464231771556
1107	23	-0.14279471669090782
1107	24	-0.03965746176405105
1107	25	0.055453722568380215
1107	26	0.26866444839036774
1107	27	0.2681964694341019
1107	28	0.15110232088500197
1107	29	0.21474665850340813
1107	30	0.12549209099809389
1107	31	0.010290955758762266
1107	32	0.01745456735703499
1107	33	0.05331833224584415
1107	34	0.011796790692443437
1107	35	0.17959034453414802
1107	36	0.020773343504430386
1107	37	-0.026414773378654388
1107	38	-0.04870863273133352
1107	39	0.10539921256771123
1107	40	0.20839914469279108
1107	41	0.24060390452902425
1107	42	0.10008124508618721
1107	43	-0.06344950180207576
1107	44	0.1425105086466374
1107	45	0.2866882414386467
1107	46	0.10589980212708383
1107	47	0.3322293734112165
1107	48	0.1926744754043881
1107	49	0.10046952577066293
1107	50	0.16882468180347843
1108	1	0.27011422953542663
1108	2	0.19266392170009183
1108	3	0.25187286036009127
1108	4	0.20941312087307296
1108	5	0.17941646373010656
1108	6	-0.0049110041558364514
1108	7	0.09918343959208187
1108	8	0.1987911122477115
1108	9	0.15545822330384595
1108	10	0.2169206398224972
1108	11	0.20463137433061718
1108	12	0.2106252110904876
1108	13	0.16423174945814623
1108	14	0.10476909280588842
1108	15	0.1433274546816392
1108	16	0.15238589160494342
1108	17	-0.07290871380074293
1108	18	0.055832138554563926
1108	19	0.18522977251725337
1108	20	-0.00186186184775039
1108	21	0.027953180974478187
1108	22	0.039693845823236984
1108	23	-0.3002643928832498
1108	24	0.08749743417536138
1108	25	0.2051287927117329
1108	26	-0.011049941851709377
1108	27	-0.09369265100974644
1108	28	-0.0669473669230527
1108	29	-0.050365560682777755
1108	30	-0.009776107641819465
1108	31	0.01847950942213572
1108	32	0.1540395899719229
1108	33	0.038330584849255445
1108	34	0.004037886994446183
1108	35	0.12955777575452032
1108	36	-0.08580215622101406
1108	37	0.03112230287935347
1108	38	0.019639332607972904
1108	39	-0.017167908644033503
1108	40	0.11317943609004288
1108	41	0.16357207762524695
1108	42	0.21227929946643087
1108	43	0.2591459007396679
1108	44	0.1733853949839741
1108	45	0.17599384881187774
1108	46	0.23635402064945765
1108	47	0.14055250095526617
1108	48	0.09118318956091542
1108	49	0.07298943978636885
1108	50	0.25760746919778654
1109	1	0.0035652934952885573
1109	2	-0.014838070163779804
1109	3	-0.12827619419902092
1109	4	-0.10503746941252246
1109	5	-0.11333239319326793
1109	6	-0.19815787724750322
1109	7	-0.09590248554417968
1109	8	-0.25924721587624333
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2211	2	-0.21479355581191584
2211	3	-0.25760825740833937
2211	4	-0.22801564723111767
2211	5	-0.36434369590259763
2211	6	-0.2354448687047892
2211	7	-0.15323461441305142
2211	8	-0.13839322162657197
2211	9	-0.1274839571906412
2211	10	-0.09619639244576916
2211	11	-0.13377370125325427
2211	12	-0.24556602831881946
2211	13	-0.19573995656502063
2211	14	-0.274857769765525
2211	15	-0.13974538555745397
2211	16	-0.2552617907592065
2211	17	-0.14927855279666602
2211	18	-0.2349853626146725
2211	19	-0.27539264136903796
2211	20	-0.14657988025088997
2211	21	-0.24433891256985873
2211	22	-0.20729783081600317
2211	23	-0.34118648885316816
2211	24	-0.2169091718026479
2211	25	-0.16684310628582466
2211	26	-0.2568564787698975
2211	27	-0.1442926807599676
2211	28	-0.013708278351828085
2211	29	-0.11300482498577935
2211	30	-0.3761538040904213
2211	31	-0.31599750987396646
2211	32	-0.08722217666397114
2211	33	-0.19052252634911326
2211	34	-0.20814447023242622
2211	35	-0.006480415307102305
2211	36	-0.046973117684650845
2211	37	0.032103211368841755
2211	38	-0.018858701242059794
2211	39	0.02681113397963486
2211	40	-0.021201855400008185
2211	41	-0.07613946139466776
2211	42	-0.15261414695346787
2211	43	-0.07305192124056248
2211	44	-0.05186615294314123
2211	45	0.04131612721406697
2211	46	-0.05627408955500357
2211	47	-0.05730458989323495
2211	48	-0.16329196252925726
2211	49	0.040414826371701906
2211	50	0.030744183067241693
2212	1	-0.05428989981025367
2212	2	-0.13908868369913563
2212	3	-0.12206131479775095
2212	4	-0.1533732051344856
2212	5	-0.05643891986860439
2212	6	-0.08098285691394125
2212	7	-0.04390075476579369
2212	8	-0.019160244703144258
2212	9	-0.11515434691020943
2212	10	-0.11578348327201127
2212	11	-0.07563747072068594
2212	12	-0.09721800058313335
2212	13	-0.13226022671595672
2212	14	-0.7581909264834437
2212	15	-0.41097067264581
2212	16	-0.3585675014419678
2212	17	-0.21579845205523895
2212	18	-0.05797804013965191
2212	19	-0.13181985285893205
2212	20	-0.07574111599361544
2212	21	-0.15789237935380385
2212	22	-0.23175950703756598
2212	23	-0.35107659874327624
2212	24	-0.23567588403609108
2212	25	0.07281023298178724
2212	26	-0.16777307743963377
2212	27	-0.2739349285796493
2212	28	-0.2414711615631191
2212	29	-0.09576380499644443
2212	30	-0.22533572149799141
2212	31	-0.08978230752568805
2212	32	-0.10557984760119865
2212	33	0.007556957651296159
2212	34	0.03472600708838769
2212	35	-0.12081416898512032
2212	36	-0.2116735249597923
2212	37	-0.060110874770032297
2212	38	-0.10828420662706151
2212	39	-0.06468306773636101
2212	40	-0.0437423974680371
2212	41	-0.009957780651753012
2212	42	0.08992332200637065
2212	43	-0.11896115504757887
2212	44	0.03861816184204514
2212	45	0.0553468971513027
2212	46	0.07172404538624022
2212	47	-0.02147073554443324
2212	48	0.022229635769576817
2212	49	0.05218933440946927
2212	50	0.1491174844685048
2213	1	-0.38572178858856176
2213	2	-0.5099655018108002
2213	3	-0.5311223362931123
2213	4	-0.651939914908958
2213	5	-0.46811838582851806
2213	6	-0.5467202221576954
2213	7	-0.6390919724815021
2213	8	-0.6255716439683567
2213	9	-0.5228257181819629
2213	10	-0.5666496752206029
2213	11	-0.594223100032579
2213	12	-0.5724468444956159
2213	13	-0.6096552822682639
2213	14	-0.714694972053536
2213	15	-0.9596018667320507
2213	16	-0.6725682252273657
2213	17	-0.8019776102289597
2213	18	-0.6989553810443496
2213	19	-0.8312862385265589
2213	20	-0.7174098227416721
2213	21	-0.8823160563610202
2213	22	-0.6936616523161945
2213	23	-0.8576932035866136
2213	24	-0.8075867573151001
2213	25	-0.8233501564431407
2213	26	-0.7318170864301834
2213	27	-0.8638367917948528
2213	28	-0.8678165952400789
2213	29	-0.8972999862834001
2213	30	-0.9800988680485645
2213	31	-0.8692455177622804
2213	32	-0.8030726319220207
2213	33	-0.9558830098493161
2213	34	-0.9693153497963536
2213	35	-1.1691570020017963
2213	36	-1.3128109146845262
2213	37	-1.0045528947699225
2213	38	-1.033111021456854
2213	39	-1.071166446394983
2213	40	-1.025724487327409
2213	41	-1.0999436420007243
2213	42	-0.9753970683410316
2213	43	-1.1957997562258527
2213	44	-1.085200764081712
2213	45	-1.0494075125289726
2213	46	-0.9962881880061687
2213	47	-1.003679097039761
2213	48	-1.0768172914243515
2213	49	-1.2500622502015766
2213	50	-1.2068107259543588
2214	1	-0.6868331478629948
2214	2	-0.9000731571291034
2214	3	-0.7737120853775785
2214	4	-0.7755523647297053
2214	5	-0.7487508824318638
2214	6	-0.725005264733511
2214	7	-0.6868618456755087
2214	8	-0.8032587666931121
2214	9	-0.9127848203779294
2214	10	-0.9028742333968509
2214	11	-0.9965207331537016
2214	12	-0.933233062111114
2214	13	-0.8916018661693741
2214	14	-0.9552283855348591
2214	15	-0.8055100321919539
2214	16	-0.8996553394769151
2214	17	-0.7295548592536107
2214	18	-0.8264571981904609
2214	19	-0.9504488196590444
2214	20	-0.9735392448428684
2214	21	-1.0234399706590622
2214	22	-0.9207778650444425
2214	23	-0.9813429425689435
2214	24	-1.1865648265030089
2214	25	-1.0749260761630666
2214	26	-1.0189412596400231
2214	27	-1.0922707129648757
2214	28	-1.0061561084436477
2214	29	-1.0551787615038606
2214	30	-0.986601408087104
2214	31	-0.8953290923004502
2214	32	-1.0203617926438682
2214	33	-1.0354980425803646
2214	34	-1.174162173464616
2214	35	-1.0752760844435656
2214	36	-0.9743140886388133
2214	37	-1.0235970084516026
2214	38	-1.040248468746043
2214	39	-0.9668812923396573
2214	40	-0.9688967965770772
2214	41	-0.8435024135345159
2214	42	-0.7248531746654052
2214	43	-0.7901629541253854
2214	44	-0.8009939827911836
2214	45	-0.7207505849291351
2214	46	-0.732638598180479
2214	47	-0.7014631164052929
2214	48	-0.7431867656286357
2214	49	-0.8789760764148582
2214	50	-0.8223790414442895
2215	1	-0.5124489572476598
2215	2	-0.49328040162341935
2215	3	-0.5389776277146723
2215	4	-0.5078694366167653
2215	5	-0.4951004463348596
2215	6	-0.4502897961844923
2215	7	-0.29576205598731065
2215	8	-0.32947515417208706
2215	9	-0.400331077396217
2215	10	-0.49202925993319013
2215	11	-0.45614297189023034
2215	12	-0.5659198112042887
2215	13	-0.5183128126988024
2215	14	-0.5131315831584828
2215	15	-0.5328358723339264
2215	16	-0.5393480610885035
2215	17	-0.4598346644783504
2215	18	-0.5416815834126325
2215	19	-0.6201972244261142
2215	20	-0.6783621850397878
2215	21	-0.6784970748402444
2215	22	-0.6097985783361892
2215	23	-0.8301634912322342
2215	24	-0.72477707090259
2215	25	-0.8244729689587871
2215	26	-0.8987736838677733
2215	27	-0.812430120470836
2215	28	-0.6788548573094602
2215	29	-0.7900988998591316
2215	30	-0.6843749660617959
2215	31	-0.7390736529298323
2215	32	-0.7399223062341349
2215	33	-0.7133093682605107
2215	34	-0.7900166941158062
2215	35	-0.7063811792943966
2215	36	-0.7885816077805501
2215	37	-0.7656692218201897
2215	38	-0.7752728577576136
2215	39	-0.7304198779243549
2215	40	-0.7881043484339898
2215	41	-0.9035331076897819
2215	42	-0.7579741050056725
2215	43	-0.8112551860620414
2215	44	-0.952805211631059
2215	45	-0.9421524531042635
2215	46	-1.0330840634242975
2215	47	-1.0222157758285881
2215	48	-1.0882797638665629
2215	49	-1.0548127447727218
2215	50	-1.0154376571757808
2216	1	-0.7314691160693023
2216	2	-0.7871435441031629
2216	3	-0.8381776438614637
2216	4	-0.7879440706788756
2216	5	-0.7470828911834744
2216	6	-0.9844667695131335
2216	7	-0.8592490454851642
2216	8	-0.9028465819746216
2216	9	-0.9821631103895001
2216	10	-0.9683126720195077
2216	11	-1.2112461431650843
2216	12	-1.2573122253515692
2216	13	-1.2123079103532604
2216	14	-1.191085963985067
2216	15	-1.3675884339552482
2216	16	-1.3014479514248762
2216	17	-1.2672696343937346
2216	18	-1.2932115592597242
2216	19	-1.2981729549002665
2216	20	-1.4296988030492912
2216	21	-1.426432146794383
2216	22	-1.3950887608622153
2216	23	-1.4219582296205573
2216	24	-1.5255824818720924
2216	25	-1.6298334348834445
2216	26	-1.5145196768842837
2216	27	-1.6188812125097982
2216	28	-1.7414260097194827
2216	29	-1.823667004562111
2216	30	-1.7199590874856696
2216	31	-1.861710199848595
2216	32	-1.6822205778545793
2216	33	-1.6898909577776564
2216	34	-1.7905045963559445
2216	35	-1.8112644626549042
2216	36	-1.9852150015896015
2216	37	-2.087084622720372
2216	38	-1.9516267775035736
2216	39	-1.97019817349587
2216	40	-2.2295801652486276
2216	41	-2.275507837959861
2216	42	-2.11019677824169
2216	43	-2.2236935983838393
2216	44	-2.2348724047130517
2216	45	-2.3545530101685976
2216	46	-2.4182112979048753
2216	47	-2.2254177744426578
2216	48	-2.3173415679566354
2216	49	-2.226554064189912
2216	50	-2.2577660694751494
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	128487.0
3	6686.0
4	12603.0
5	9802.0
6	8333.0
7	7135.0
8	6345.0
9	5936.0
10	5737.0
11	5755.0
12	5780.0
13	6349.0
14	6437.0
15	7029.0
16	7500.0
17	8204.0
18	8785.0
19	10183.0
20	11976.0
21	15065.0
22	18497.0
23	21254.0
24	23302.0
25	25666.0
26	28603.0
27	32186.0
28	36860.0
29	42786.0
30	50887.0
31	60925.0
32	74435.0
33	93533.0
34	119756.0
35	157987.0
36	209680.0
37	292087.0
38	463803.0
39	853366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.25110658854856	16.255976270862398	12.173166734320386	25.31975040626866
2	16.725245332440135	18.59930079691694	38.218968403544025	26.4564854670989
3	20.42513715945692	22.69794158624851	27.2256412494726	29.651280004821974
4	13.563540036006025	14.81883190686792	34.729118219555005	36.88850983757104
5	15.401742725596229	36.534749904051715	32.73883501467974	15.324672355672309
6	35.438647638727694	33.661807192538774	16.493277250403725	14.40626791832981
7	31.297658913826865	30.127669799379	19.60517714643422	18.969494140359917
8	27.991531935123877	34.028248350863755	18.622228947305548	19.35799076670682
9	27.437235241201023	15.564198966345637	17.622851199062605	39.37571459339073
10	15.6909849669822	28.22106173501147	32.091141175761884	23.99681212224445
11	37.27230583178891	21.913446354833315	21.500686029679827	19.313561783697942
12	24.705237105291953	23.587644957695137	28.494773697717935	23.21234423929498
13	29.56382736733208	20.473513156432386	24.434586223025928	25.528073253209605
14	23.221500258113615	19.89410244150204	25.244238492131778	31.640158808252572
15	25.10953792738834	27.611659440308365	22.0747444230437	25.204058209259593
16	25.352174608527534	26.6437758076143	23.66001222907848	24.344037354779694
17	24.17235304626984	26.755148903049246	24.900872103893516	24.171625946787398
18	24.126048942090225	26.37863433591353	25.435157755073494	24.06015896692275
19	25.191021024951677	25.92532572088665	25.32218891084254	23.56146434331913
20	25.25937058481314	25.341164085658086	25.308058841454965	24.0914064880738
21	25.490577714762058	25.203335938850124	25.158036657093096	24.14804968929472
22	25.230024883935577	24.89023881305186	25.1681007686833	24.711635534329268
23	25.00135904237546	24.935848006085738	25.37423871986172	24.688554231677077
24	24.615743187411212	25.156622183311505	25.20566077072313	25.02197385855415
25	25.003298885236546	24.960075695995517	25.289652513958355	24.74697290480958
26	24.53903229336802	25.442404318896838	25.548381868280295	24.470181519454844
27	24.845162961567446	25.33952635311849	25.10631843166807	24.708992253645995
28	24.78703531474575	25.42624977441833	25.04121087045602	24.7455040403799
29	24.79274212122209	25.561579928965884	25.105520815836	24.540157133976024
30	24.905036724674382	25.514955850042533	25.2182908549881	24.361716570294988
31	24.98036828678505	25.072017572287635	25.095561778612442	24.852052362314865
32	24.72301317349347	25.524370685930176	24.97870663771231	24.773909502864043
33	24.78208406404781	25.27374742699059	25.036537166460942	24.907631342500665
34	25.08460950140032	25.30134831624063	25.085128843608402	24.528913338750648
35	25.022281254176654	25.281865655982976	25.13034620256101	24.56550688727936
36	24.934307863354814	25.423004037612362	24.947153710077895	24.695534388954925
37	24.8975247349909	25.032115495284994	25.21719077539098	24.85316899433313
38	24.97993611409444	25.34437522201841	25.03865630476887	24.637032359118283
39	25.248957548154642	25.388493575283494	24.925877977648614	24.436670898913246
40	25.16756702854266	25.07314502485719	25.200772272963036	24.558515673637114
41	24.765990005002692	25.382515505747904	25.160942998741536	24.69055149050787
42	25.11091832340238	25.18205975741983	25.001453985512768	24.705567933665026
43	24.767135845328212	25.014176447088097	25.178200882505468	25.040486825078222
44	24.89830070174333	25.12477225623065	25.08326516454152	24.893661877484497
45	24.901199315863458	25.258429082102964	24.930033850289572	24.910337751744006
46	24.858745719449253	25.12251037491619	24.978720561424108	25.040023344210454
47	24.781372898235716	25.140031078401133	25.030817049019618	25.047778974343533
48	24.691370844777662	25.358698643295135	25.147136451037298	24.802794060889894
49	24.95830705529776	25.106144802764163	25.041762173336036	24.893785968602046
50	25.002812462897122	25.127149284145982	25.137256843111643	24.73278140984525
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1672.0
1	1277.0
2	882.0
3	864.5
4	847.0
5	785.0
6	723.0
7	710.0
8	697.0
9	729.5
10	762.0
11	908.5
12	1055.0
13	1498.5
14	1942.0
15	2562.5
16	3183.0
17	3425.5
18	3668.0
19	3770.0
20	3872.0
21	4306.0
22	4740.0
23	5886.5
24	7033.0
25	9395.0
26	11757.0
27	15526.0
28	19295.0
29	24809.5
30	30324.0
31	38723.0
32	47122.0
33	57080.0
34	67038.0
35	78447.0
36	89856.0
37	99488.0
38	109120.0
39	120669.0
40	132218.0
41	144772.5
42	157327.0
43	165280.5
44	173234.0
45	184342.5
46	195451.0
47	206842.5
48	218234.0
49	221858.0
50	225482.0
51	227772.0
52	230062.0
53	226167.0
54	222272.0
55	215821.5
56	209371.0
57	199335.5
58	189300.0
59	173616.0
60	157932.0
61	140122.0
62	122312.0
63	105360.0
64	88408.0
65	74451.0
66	60494.0
67	51059.0
68	41624.0
69	33216.5
70	24809.0
71	19919.5
72	15030.0
73	12278.5
74	9527.0
75	7257.0
76	4987.0
77	3806.0
78	2625.0
79	2045.0
80	1465.0
81	1137.5
82	810.0
83	655.0
84	500.0
85	380.0
86	260.0
87	214.0
88	168.0
89	129.5
90	91.0
91	73.0
92	55.0
93	44.5
94	34.0
95	26.0
96	18.0
97	17.5
98	17.0
99	26.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08609771121277347
2	0.09478361375071805
3	0.10222372947047138
4	0.10385017337199887
5	0.09578716424314991
6	0.05661408984891374
7	0.052184625606455946
8	0.059278689432267266
9	0.060005398409545496
10	0.04491753583367362
11	0.04778976655339234
12	0.05730619363679778
13	0.05041976094735166
14	0.05111186473523569
15	0.05090423359887049
16	0.0533612020458588
17	0.05370725393980081
18	0.055091461515568874
19	0.06360433810654245
20	0.06851827500051907
21	0.07879601625059694
22	0.08080311723546063
23	0.05803290261407601
24	0.07675431007633904
25	0.08308705973547793
26	0.08083772242485483
27	0.075716154394513
28	0.0954757175386021
29	0.08287942859911272
30	0.07986877712181718
31	0.053845674697377624
32	0.05246146712160956
33	0.05484922518980946
34	0.05090423359887049
35	0.05751382477316298
36	0.05699474693224996
37	0.05972855689439188
38	0.05073120765189948
39	0.05526448746253988
40	0.056821720985278955
41	0.04481372026549101
42	0.03903465363665935
43	0.03965754704575498
44	0.03726978897755507
45	0.02917217465931191
46	0.027718756704755446
47	0.03173295867448283
48	0.02612691799262217
49	0.026369154318381584
50	0.028134018977485863
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2889740.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.878033424669184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.38612163861251	44.789565348051305
2	12.075736874919263	13.978398049481747
3	4.360164983878547	7.570733240219843
4	2.088587429446818	4.835333322074674
5	1.1301352996224574	3.270500432297356
6	0.6557841732044061	2.2773298977656196
7	0.44051271046058654	1.7847206566020613
8	0.3233875563379184	1.4973628635878504
9	0.23303572357164629	1.2138884458219539
>10	1.201656874762616	12.397204879138407
>50	0.07239083126367185	2.876983327596933
>100	0.03122192303663979	3.023054941803255
>500	0.0012037913170786525	0.44820688553861554
>1k	6.018956581626491E-5	0.03671771002045132
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	3.460518939420155E-5
9	0.0	0.0	0.0	3.460518939420155E-5	3.460518939420155E-5
10	0.0	0.0	0.0	3.460518939420155E-5	3.460518939420155E-5
11	0.0	0.0	0.0	3.460518939420155E-5	3.460518939420155E-5
12	0.0	0.0	0.0	3.460518939420155E-5	2.0763113636520933E-4
13	0.0	0.0	0.0	3.460518939420155E-5	2.4223632575941088E-4
14	0.0	0.0	0.0	3.460518939420155E-5	2.4223632575941088E-4
15	0.0	0.0	0.0	3.460518939420155E-5	2.4223632575941088E-4
16	0.0	0.0	0.0	3.460518939420155E-5	2.4223632575941088E-4
17	0.0	0.0	0.0	3.460518939420155E-5	2.768415151536124E-4
18	0.0	0.0	0.0	3.460518939420155E-5	3.1144670454781397E-4
19	0.0	0.0	0.0	3.460518939420155E-5	3.1144670454781397E-4
20	0.0	0.0	0.0	3.460518939420155E-5	3.1144670454781397E-4
21	0.0	0.0	0.0	3.460518939420155E-5	3.806570833362171E-4
22	0.0	0.0	0.0	6.92103787884031E-5	3.806570833362171E-4
23	0.0	0.0	0.0	1.384207575768062E-4	3.806570833362171E-4
24	0.0	0.0	0.0	4.1526227273041866E-4	3.806570833362171E-4
25	0.0	0.0	0.0	4.8447265151882175E-4	3.806570833362171E-4
26	0.0	0.0	0.0	8.651297348550389E-4	3.806570833362171E-4
27	0.0	0.0	0.0	0.0013496023863738605	3.806570833362171E-4
28	0.0	0.0	0.0	0.0023531528788057056	3.806570833362171E-4
29	0.0	0.0	0.0	0.003875781212150574	3.806570833362171E-4
30	0.0	0.0	0.0	0.006540380795504094	3.806570833362171E-4
31	0.0	0.0	0.0	0.013253787537979195	3.806570833362171E-4
32	0.0	0.0	0.0	0.021109165530462947	3.806570833362171E-4
33	0.0	0.0	0.0	0.03138690678054081	3.806570833362171E-4
34	0.0	0.0	0.0	0.04256438295486791	4.8447265151882175E-4
35	0.0	0.0	0.0	0.057340798826191974	4.8447265151882175E-4
36	0.0	0.0	0.0	0.07671970488694485	4.8447265151882175E-4
37	0.0	0.0	0.0	0.10211991390228879	4.8447265151882175E-4
38	0.0	0.0	0.0	0.13091143147826448	5.190778409130233E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2200	0.0	20.805897	1
GTATCAA	4140	0.0	20.730513	1
GCGATAC	185	2.1827873E-11	17.838568	14
TATCAAC	5095	0.0	16.542185	2
TCAACGC	5195	0.0	16.265837	4
CGTGCCG	230	1.8189894E-12	16.258438	10
ATCAACG	5230	0.0	16.241415	3
CAACGCA	5235	0.0	16.139874	5
GGACCGT	205	1.05501385E-10	16.09599	6
CGTTACG	195	8.913048E-10	15.798255	24
AACGCAG	5340	0.0	15.736292	6
TGCGATA	215	2.1645974E-10	15.347339	13
GTACATG	9520	0.0	15.27954	1
TACATGG	9655	0.0	14.49583	2
CGATACT	250	1.382432E-10	14.081065	15
GCACCGT	375	0.0	14.078626	6
ACATGGG	9865	0.0	13.986488	3
GTGCCGA	340	0.0	13.587145	11
AGCACCG	560	0.0	13.359077	5
GTATAGT	300	1.2732926E-11	13.203742	1
>>END_MODULE