FastQCFastQC Report
Mon 6 Feb 2017
SRR2077105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889740
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.021.6631051
GTATCAA45150.021.100091
CGTGCCG2950.020.13984110
CGTTACG2150.017.39600624
GCGATAC1903.274181E-1117.37268614
TGCGATA2201.8189894E-1116.0047613
TATCAAC59550.015.7394472
TTACGCG850.002988104715.52408643
ATCAACG60950.015.2696233
TCAACGC60850.015.2229274
CAACGCA61750.014.9300515
GTACATG88950.014.7171981
GCACCGT4200.014.6677286
GCCGTAC1809.057294E-814.6677271
GTTACGG3450.014.6672225
GTGCCGA3500.014.46094211
AACGCAG63950.014.4154296
TACATGG91700.014.1321292
GTATTAG3750.014.0810181
CCGATCG1901.7524144E-713.8967059
Produced by FastQC (version 0.11.5)