##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077105_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2889740 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.474923003453597 31.0 31.0 34.0 27.0 34.0 2 30.585221853869207 31.0 31.0 34.0 27.0 34.0 3 30.642442226636305 31.0 31.0 34.0 27.0 34.0 4 34.17871330984794 37.0 35.0 37.0 30.0 37.0 5 33.6805152020597 37.0 35.0 37.0 28.0 37.0 6 33.596963048578765 37.0 35.0 37.0 28.0 37.0 7 33.417073854395206 37.0 35.0 37.0 27.0 37.0 8 33.34319488950563 37.0 35.0 37.0 27.0 37.0 9 34.77998643476576 39.0 35.0 39.0 27.0 39.0 10 34.6296068158381 39.0 35.0 39.0 27.0 39.0 11 34.63679708209043 39.0 35.0 39.0 27.0 39.0 12 34.54397489047457 39.0 35.0 39.0 27.0 39.0 13 34.51225231335691 39.0 35.0 39.0 27.0 39.0 14 35.56840061735658 40.0 36.0 41.0 26.0 41.0 15 35.57030528698083 40.0 36.0 41.0 27.0 41.0 16 35.532457937392294 40.0 36.0 41.0 26.0 41.0 17 35.506231356454215 40.0 36.0 41.0 26.0 41.0 18 35.42850567871158 40.0 36.0 41.0 26.0 41.0 19 35.48024908815326 40.0 36.0 41.0 26.0 41.0 20 35.445601680428 40.0 35.0 41.0 25.0 41.0 21 35.41105670406334 40.0 35.0 41.0 25.0 41.0 22 35.31840684629067 39.0 35.0 41.0 25.0 41.0 23 35.266535397648234 39.0 35.0 41.0 25.0 41.0 24 35.25180569878259 39.0 35.0 41.0 25.0 41.0 25 35.179115768200596 39.0 35.0 41.0 25.0 41.0 26 35.116246444316786 39.0 35.0 41.0 24.0 41.0 27 35.01051547890122 39.0 35.0 41.0 24.0 41.0 28 34.97447452019905 39.0 35.0 41.0 24.0 41.0 29 34.934029705094574 39.0 35.0 41.0 24.0 41.0 30 34.85959844138227 39.0 35.0 41.0 23.0 41.0 31 34.78032452746614 39.0 34.0 41.0 23.0 41.0 32 34.71518302684671 39.0 34.0 41.0 23.0 41.0 33 34.594946604192764 39.0 34.0 41.0 22.0 41.0 34 34.55033567033713 39.0 34.0 41.0 22.0 41.0 35 34.48361548097753 39.0 34.0 41.0 21.0 41.0 36 34.408166132593244 39.0 34.0 41.0 20.0 41.0 37 34.3270481081343 39.0 34.0 41.0 19.0 41.0 38 34.1573003107546 39.0 34.0 41.0 18.0 41.0 39 34.00875061424211 39.0 33.0 41.0 16.0 41.0 40 33.987147632658996 39.0 33.0 41.0 16.0 41.0 41 33.87932305328507 38.0 33.0 41.0 15.0 41.0 42 34.01107712112508 39.0 33.0 41.0 15.0 41.0 43 33.999241800300375 39.0 34.0 41.0 15.0 41.0 44 33.92165800383425 39.0 33.0 41.0 14.0 41.0 45 33.78172153896198 39.0 33.0 41.0 9.0 41.0 46 33.6754427733983 39.0 33.0 41.0 8.0 41.0 47 33.54184736343062 38.0 33.0 41.0 2.0 41.0 48 33.39160962577948 38.0 33.0 41.0 2.0 41.0 49 33.14271007080222 38.0 33.0 41.0 2.0 41.0 50 33.01240561434593 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6022655279172398 1101 2 0.7364338078315988 1101 3 0.6763794990521212 1101 4 0.7051474679839629 1101 5 1.0478785428081352 1101 6 1.0804838727567088 1101 7 1.165401035369804 1101 8 1.0763473711543483 1101 9 1.3373961453981806 1101 10 1.4282913558119503 1101 11 1.463690490765238 1101 12 1.3957145499275327 1101 13 1.4284037832787106 1101 14 1.6194006085210688 1101 15 1.6144351632042842 1101 16 1.5904441750662883 1101 17 1.5030441454518453 1101 18 1.7365389844360166 1101 19 1.5931565573073527 1101 20 1.5629276212241408 1101 21 1.5314455674487562 1101 22 1.8403708979981772 1101 23 1.7776704172875384 1101 24 1.7840150498899447 1101 25 1.6768890533069865 1101 26 1.762520438427444 1101 27 1.8941668672332597 1101 28 1.838163812541275 1101 29 1.795129445227225 1101 30 1.8219828255931603 1101 31 1.8978145653723288 1101 32 1.7820002742138996 1101 33 1.842502254324991 1101 34 1.9235956074087 1101 35 1.8202872969767014 1101 36 1.9008875917029329 1101 37 1.7827460573000167 1101 38 2.0381499604857893 1101 39 2.126587803096875 1101 40 2.2180952770197635 1101 41 2.206707872584282 1101 42 2.0481805730148466 1101 43 2.1185415309113083 1101 44 2.163611701708824 1101 45 2.025737454830818 1101 46 2.03526426301989 1101 47 2.0245413812471966 1101 48 1.914068018716911 1101 49 2.254755351630976 1101 50 2.0680611415239127 1102 1 0.2560467229236778 1102 2 0.3559861233189672 1102 3 0.40452380216187933 1102 4 0.33801339293878385 1102 5 0.4325795549849687 1102 6 0.49869921721571586 1102 7 0.5037878711656703 1102 8 0.5423945667040755 1102 9 0.5536190250825967 1102 10 0.5961095655463282 1102 11 0.5469544362470558 1102 12 0.5967547771653656 1102 13 0.6350570448029984 1102 14 0.6661650677682545 1102 15 0.6906053081215475 1102 16 0.7142970610103774 1102 17 0.727062085902304 1102 18 0.6283470842127912 1102 19 0.6407787685062516 1102 20 0.7177496340033187 1102 21 0.7889305973888767 1102 22 0.8454937883122255 1102 23 0.8392248232684736 1102 24 0.7650706900888409 1102 25 0.7433640913964936 1102 26 0.8343339120718696 1102 27 0.6753751607054852 1102 28 0.7599961833027109 1102 29 0.7528899597012355 1102 30 0.7677466234412549 1102 31 0.7969334872918026 1102 32 0.8780064866411195 1102 33 0.8327244726103089 1102 34 0.868774775699336 1102 35 0.8561180024296959 1102 36 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42 0.056290054957869984 1107 43 0.10428823481689875 1107 44 0.04981314213473098 1107 45 0.025853983951130033 1107 46 -0.010817230098169262 1107 47 0.07159363542429276 1107 48 0.05344062593314902 1107 49 0.09339747873463722 1107 50 0.10782224212990599 1108 1 0.08348048451844647 1108 2 0.08806537607805964 1108 3 0.08386649827867743 1108 4 0.12459140588208584 1108 5 0.07653956270597462 1108 6 -0.006867386028382327 1108 7 0.053489371459448876 1108 8 0.04694497704173983 1108 9 0.06448512497632919 1108 10 0.02310456598116417 1108 11 0.05117495376674697 1108 12 0.04792305697132804 1108 13 -0.03558758517191052 1108 14 0.11322217565662385 1108 15 0.05340447068908105 1108 16 0.042387426506799386 1108 17 0.007460216315230639 1108 18 0.1265137689092839 1108 19 0.17352533700581318 1108 20 0.15406833560118827 1108 21 0.1486612953977442 1108 22 0.16270697087377073 1108 23 0.22513398263306073 1108 24 0.1999463160950583 1108 25 0.02306627046025511 1108 26 0.1573945389583784 1108 27 0.14834786566750324 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77040.0 3 12957.0 4 21330.0 5 12892.0 6 9873.0 7 7746.0 8 6554.0 9 5876.0 10 5466.0 11 5466.0 12 5584.0 13 5963.0 14 6160.0 15 6671.0 16 7234.0 17 7655.0 18 8256.0 19 9227.0 20 10441.0 21 12723.0 22 15675.0 23 18732.0 24 21533.0 25 25210.0 26 27971.0 27 32513.0 28 37168.0 29 43394.0 30 51408.0 31 61163.0 32 75031.0 33 93032.0 34 118731.0 35 154468.0 36 200562.0 37 272806.0 38 425753.0 39 969476.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.654980996251155 16.814969617879825 13.316362672436139 27.213686713432878 2 16.049229755470897 18.59592340745991 37.25386506600514 28.100981771064053 3 19.15111998081486 22.3092274487263 27.772423580415794 30.767228990043037 4 13.171478880063345 14.847355612833923 34.23088795926899 37.75027754783375 5 15.077002968643166 36.30527691468829 33.138435706805375 15.479284409863164 6 35.549522518543334 33.84822223483752 16.21585041822031 14.386404828398828 7 30.991319734701015 29.991446769727727 19.720439866568224 19.29679362900303 8 28.072161950719682 33.81711310657094 18.490054625135873 19.620670317573506 9 27.141181206947817 14.991150277053618 17.66527935042722 40.20238916557134 10 15.322494378871829 28.088054821361496 32.39919616568957 24.190254634077103 11 37.4105745420153 21.499810114246486 21.505141455366118 19.584473888372102 12 24.493504155747946 23.337960446833776 28.78733855585595 23.38119684156233 13 29.50432297011521 19.985652420184664 24.745143496571675 25.764881113128453 14 22.989191812948913 19.469900975153426 25.525331623485386 32.01557558841227 15 24.99717828854025 27.388847072933103 22.30086853317398 25.313106105352666 16 25.165605792428735 26.316536619550558 23.919736415685428 24.59812117233528 17 23.993004569327788 26.51003005015977 25.149930526966717 24.347034853545722 18 24.06858308571951 25.96365114460947 25.827720330139453 24.140045439531562 19 25.029247378988213 25.643797218633985 25.820406724664014 23.50654867771379 20 25.08012346127813 24.980585835189924 25.747008245177618 24.192282458354324 21 25.284861619847533 24.810207656781216 25.726452524217393 24.178478199153865 22 24.902221799077353 24.592329729870084 25.71172871028329 24.793719760769278 23 24.758129765349953 24.58994145260278 25.835428186048542 24.816500595998725 24 24.43534486190741 24.796289327489074 25.731608397757615 25.0367574128459 25 24.73079044184468 24.642926258131677 25.780279302861054 24.846003997162587 26 24.37471568543956 25.055374263551723 26.03296629491997 24.536943756088743 27 24.586495396811053 25.101668974059915 25.61064565121284 24.701189977916187 28 24.52828882298182 25.0320313205905 25.660090999761476 24.7795888566662 29 24.64866526553965 25.15721136719971 25.601090068049633 24.593033299211008 30 24.658318609069408 25.14588099102143 25.69118648949546 24.5046139104137 31 24.70681252825601 24.67209182052129 25.719771002566493 24.90132464865621 32 24.458315895942654 25.123965568985085 25.52456690367672 24.89315163139554 33 24.63938775616164 24.928549053406922 25.50746007763811 24.924603112793328 34 24.763284321869637 24.98726255745694 25.5900066456222 24.659446475051226 35 24.806154595906488 24.958932671733425 25.553188852946086 24.681723879414 36 24.640378907028776 25.080438827725228 25.48007166116563 24.799110604080365 37 24.679586467609756 24.733546844287105 25.698291923907217 24.888574764195923 38 24.84481572916966 24.972078079622552 25.50916188553172 24.67394430567607 39 25.042950509660923 25.0381051661053 25.38326667524985 24.53567764898393 40 24.869784186107037 24.731487204760516 25.701261908041506 24.69746670109094 41 24.514031140629104 25.06854904436247 25.56440703129948 24.853012783708948 42 24.91457147306674 24.853523905106332 25.476527486978938 24.755377134847986 43 24.56886169520619 24.681370174005732 25.610715818325282 25.139052312462795 44 24.6825314976789 24.829507976597277 25.457454341083714 25.03050618464011 45 24.649905213302944 25.007734511424584 25.346772580447567 24.995587694824902 46 24.712212090968112 24.825237020128544 25.37392356386358 25.088627325039763 47 24.549681815869516 24.848296298654628 25.367177461579644 25.23484442389622 48 24.51437462192993 25.12728070551854 25.493238688351223 24.865105984200305 49 24.79428044440061 24.90259487461653 25.35703100803361 24.94609367294925 50 24.857753104653987 24.83567464236698 25.495848349340793 24.81072390363824 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 571.0 1 407.0 2 243.0 3 343.5 4 444.0 5 493.5 6 543.0 7 493.0 8 443.0 9 524.5 10 606.0 11 942.0 12 1278.0 13 2236.5 14 3195.0 15 4151.5 16 5108.0 17 5715.0 18 6322.0 19 6384.5 20 6447.0 21 6462.5 22 6478.0 23 7395.0 24 8312.0 25 10362.0 26 12412.0 27 15797.0 28 19182.0 29 24520.0 30 29858.0 31 37805.5 32 45753.0 33 55643.0 34 65533.0 35 76962.0 36 88391.0 37 97961.5 38 107532.0 39 119046.5 40 130561.0 41 142855.0 42 155149.0 43 163491.0 44 171833.0 45 183360.5 46 194888.0 47 206124.0 48 217360.0 49 222327.5 50 227295.0 51 229335.5 52 231376.0 53 227201.5 54 223027.0 55 217010.0 56 210993.0 57 200894.5 58 190796.0 59 174328.5 60 157861.0 61 140216.0 62 122571.0 63 105326.0 64 88081.0 65 74113.0 66 60145.0 67 50585.0 68 41025.0 69 32716.5 70 24408.0 71 19436.0 72 14464.0 73 11921.5 74 9379.0 75 6987.5 76 4596.0 77 3462.0 78 2328.0 79 1864.5 80 1401.0 81 1086.0 82 771.0 83 578.0 84 385.0 85 305.0 86 225.0 87 157.0 88 89.0 89 67.0 90 45.0 91 33.0 92 21.0 93 14.0 94 7.0 95 6.0 96 5.0 97 3.5 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001626443901527473 2 0.009274190757646017 3 1.0381556818260465E-4 4 0.00512156803034183 5 0.042045305113954885 6 0.028791517575975693 7 0.026749811401717803 8 0.03322098181843349 9 0.03284032473509728 10 0.037546630492708684 11 0.040176624886668004 12 0.03356703371237551 13 0.05010831424280385 14 0.04754753022763293 15 0.04920857931855461 16 0.053845674697377624 17 0.05429554215950224 18 0.05211541522766754 19 0.05007370905340965 20 0.04792818731096916 21 0.04567885000034605 22 0.04550582405337505 23 0.045367403295798234 24 0.04176846359880128 25 0.04121478056849405 26 0.04201069992456069 27 0.041491622083647664 28 0.040176624886668004 29 0.03851557579574633 30 0.03751202530331448 31 0.033705454469952316 32 0.028826122765369895 33 0.02405060662897008 34 0.021455217424404965 35 0.020140220227425305 36 0.020347851363790515 37 0.020174825416819506 38 0.014984047007689273 39 0.01273470969706617 40 0.010589187954625675 41 0.007024853447022915 42 0.006574985984898296 43 0.00622893409095628 44 0.005606040681860652 45 0.003252887803054946 46 0.003875781212150574 47 0.0021109165530462945 48 0.0022147321212288994 49 1.7302594697100776E-4 50 0.0015918387121332713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2889740.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.628461452851454 #Duplication Level Percentage of deduplicated Percentage of total 1 76.45282032774236 43.29405588891336 2 12.60123436243902 14.271770287034503 3 4.511741505576271 7.664789398012675 4 2.1145627742887374 4.789777462137775 5 1.1663521013066633 3.302436250464833 6 0.7235248311290139 2.458325880586213 7 0.4854981919379761 1.9245110953312126 8 0.32892540068765547 1.4901231498963692 9 0.24767336706392598 1.2622825547709715 >10 1.2518403944133156 12.618376377771812 >50 0.07944437006937617 3.080128717925722 >100 0.0351531284208558 3.3164733535072917 >500 0.0010448581860635203 0.3747467746057995 >1k 1.8438673866246353E-4 0.15220280904149444 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.384207575768062E-4 2 0.0 0.0 0.0 0.0 1.384207575768062E-4 3 0.0 0.0 0.0 0.0 1.7302594697100778E-4 4 0.0 0.0 0.0 0.0 1.7302594697100778E-4 5 0.0 0.0 0.0 0.0 1.7302594697100778E-4 6 0.0 0.0 0.0 0.0 1.7302594697100778E-4 7 0.0 0.0 0.0 3.460518939420155E-5 1.7302594697100778E-4 8 0.0 0.0 0.0 3.460518939420155E-5 1.7302594697100778E-4 9 0.0 0.0 0.0 3.460518939420155E-5 1.7302594697100778E-4 10 0.0 0.0 0.0 3.460518939420155E-5 1.7302594697100778E-4 11 0.0 0.0 0.0 3.460518939420155E-5 1.7302594697100778E-4 12 0.0 0.0 0.0 3.460518939420155E-5 3.4605189394201557E-4 13 0.0 0.0 0.0 3.460518939420155E-5 4.1526227273041866E-4 14 0.0 0.0 0.0 3.460518939420155E-5 4.1526227273041866E-4 15 0.0 0.0 0.0 3.460518939420155E-5 4.1526227273041866E-4 16 0.0 0.0 0.0 3.460518939420155E-5 4.498674621246202E-4 17 0.0 0.0 0.0 3.460518939420155E-5 4.8447265151882175E-4 18 0.0 0.0 0.0 6.92103787884031E-5 5.190778409130233E-4 19 0.0 0.0 0.0 6.92103787884031E-5 5.190778409130233E-4 20 0.0 0.0 0.0 6.92103787884031E-5 5.882882197014264E-4 21 0.0 0.0 0.0 6.92103787884031E-5 6.574985984898295E-4 22 0.0 0.0 0.0 1.0381556818260467E-4 6.574985984898295E-4 23 0.0 0.0 0.0 1.7302594697100778E-4 6.574985984898295E-4 24 0.0 0.0 0.0 4.498674621246202E-4 6.574985984898295E-4 25 0.0 0.0 0.0 5.190778409130233E-4 6.574985984898295E-4 26 0.0 0.0 0.0 8.651297348550389E-4 6.574985984898295E-4 27 0.0 0.0 0.0 0.001314997196979659 6.921037878840311E-4 28 0.0 0.0 0.0 0.0023185476894115042 6.921037878840311E-4 29 0.0 0.0 0.0 0.003737360454573768 6.921037878840311E-4 30 0.0 0.0 0.0 0.006263539280350481 6.921037878840311E-4 31 0.0 0.0 0.0 0.012734709697066172 6.921037878840311E-4 32 0.0 0.0 0.0 0.02048627212136732 6.921037878840311E-4 33 0.0 0.0 0.0 0.03072940818205098 6.921037878840311E-4 34 0.0 0.0 0.0 0.04187227916698388 6.921037878840311E-4 35 0.0 0.0 0.0 0.05633724833376013 6.921037878840311E-4 36 0.0 0.0 0.0 0.07557773363693619 6.921037878840311E-4 37 0.0 0.0 0.0 0.10063189075833812 6.921037878840311E-4 38 0.0 0.0 0.0 0.1291811720085544 6.921037878840311E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2275 0.0 21.663105 1 GTATCAA 4515 0.0 21.10009 1 CGTGCCG 295 0.0 20.139841 10 CGTTACG 215 0.0 17.396006 24 GCGATAC 190 3.274181E-11 17.372686 14 TGCGATA 220 1.8189894E-11 16.00476 13 TATCAAC 5955 0.0 15.739447 2 TTACGCG 85 0.0029881047 15.524086 43 ATCAACG 6095 0.0 15.269623 3 TCAACGC 6085 0.0 15.222927 4 CAACGCA 6175 0.0 14.930051 5 GTACATG 8895 0.0 14.717198 1 GCACCGT 420 0.0 14.667728 6 GCCGTAC 180 9.057294E-8 14.667727 1 GTTACGG 345 0.0 14.66722 25 GTGCCGA 350 0.0 14.460942 11 AACGCAG 6395 0.0 14.415429 6 TACATGG 9170 0.0 14.132129 2 GTATTAG 375 0.0 14.081018 1 CCGATCG 190 1.7524144E-7 13.896705 9 >>END_MODULE