FastQCFastQC Report
Mon 6 Feb 2017
SRR2077104_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077104_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912213
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42850.019.156361
GGTATCA24400.018.2186641
TAATCCG800.001985209816.5050285
GTATTAG3450.015.3090111
GTAGCGA1756.434311E-815.08279333
GTACATG73600.014.9203051
ATCATAC9950.014.8193493
ATAACGC1351.9512689E-514.6718923
ATCAACG54800.014.4175443
TCAACGC54750.014.3101214
TATCAAC56550.014.1654792
TATCATA7800.014.1071042
CAACGCA55600.014.0114635
TACATGG73850.014.005862
AACGCAG57400.013.7578636
TATAACG1302.3259265E-413.542822
TCATACT9000.013.4492344
ACATGGG74400.013.3703543
GTCCTAC4300.013.3063781
CGAACAT1353.215878E-413.03406410
Produced by FastQC (version 0.11.5)