##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077104_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2912213 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.32345710976498 31.0 28.0 31.0 10.0 34.0 2 27.478847872734583 31.0 28.0 34.0 10.0 34.0 3 27.58447819579131 31.0 28.0 34.0 10.0 34.0 4 30.830916557271053 35.0 32.0 37.0 10.0 37.0 5 30.792566340442818 35.0 32.0 37.0 10.0 37.0 6 30.83394998923499 35.0 32.0 37.0 8.0 37.0 7 30.63370639441552 35.0 31.0 37.0 8.0 37.0 8 30.518348760890774 35.0 31.0 37.0 8.0 37.0 9 31.698479815865117 37.0 31.0 39.0 7.0 39.0 10 31.646831807975584 37.0 31.0 39.0 2.0 39.0 11 31.64080958363966 37.0 31.0 39.0 2.0 39.0 12 31.550868016865525 37.0 31.0 39.0 2.0 39.0 13 31.476038325493363 37.0 31.0 39.0 2.0 39.0 14 32.25589577410718 38.0 31.0 40.0 2.0 41.0 15 32.294528250509146 38.0 31.0 40.0 2.0 41.0 16 32.256017674531364 38.0 31.0 40.0 2.0 41.0 17 32.09240120829074 38.0 31.0 40.0 2.0 41.0 18 32.06214380610209 38.0 31.0 40.0 2.0 41.0 19 32.2010532883412 38.0 31.0 40.0 2.0 41.0 20 32.181476766980985 38.0 31.0 40.0 2.0 41.0 21 32.132674018006234 38.0 31.0 40.0 2.0 41.0 22 32.06321069234977 38.0 31.0 40.0 2.0 41.0 23 32.01476162629588 38.0 31.0 40.0 2.0 41.0 24 32.01524236036306 38.0 31.0 40.0 2.0 41.0 25 31.919061895541294 38.0 30.0 40.0 2.0 41.0 26 31.865599803311092 38.0 30.0 40.0 2.0 41.0 27 31.791410518392713 38.0 30.0 40.0 2.0 41.0 28 31.686741663470357 38.0 30.0 40.0 2.0 41.0 29 31.669832872801543 38.0 30.0 40.0 2.0 41.0 30 31.487745230173754 38.0 30.0 40.0 2.0 41.0 31 31.47103697428725 38.0 30.0 40.0 2.0 41.0 32 31.344520816300182 38.0 30.0 40.0 2.0 41.0 33 31.287428495099775 38.0 30.0 40.0 2.0 41.0 34 31.207198443245737 38.0 30.0 40.0 2.0 41.0 35 31.12867018998954 38.0 30.0 40.0 2.0 41.0 36 31.0348127008567 38.0 30.0 40.0 2.0 41.0 37 30.943494861124513 37.0 30.0 40.0 2.0 41.0 38 30.851804109108777 37.0 29.0 40.0 2.0 41.0 39 30.74524013181728 37.0 29.0 40.0 2.0 41.0 40 30.684766189835702 37.0 29.0 40.0 2.0 41.0 41 30.563451917837053 37.0 29.0 40.0 2.0 41.0 42 30.41365483912063 37.0 28.0 40.0 2.0 41.0 43 30.325441854699502 37.0 28.0 40.0 2.0 41.0 44 30.17874276366461 37.0 28.0 40.0 2.0 41.0 45 30.031850005476933 37.0 28.0 40.0 2.0 41.0 46 29.880317133396492 37.0 27.0 40.0 2.0 41.0 47 29.72104993693799 37.0 27.0 40.0 2.0 41.0 48 29.521955296539094 36.0 27.0 40.0 2.0 41.0 49 29.336333571754537 36.0 26.0 40.0 2.0 41.0 50 29.12071507132205 36.0 26.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 2.108832113722798 1101 2 2.4301890260956895 1101 3 2.5355932972426096 1101 4 2.8773990617051943 1101 5 2.986338377400223 1101 6 2.9453200740725087 1101 7 3.0839071260537807 1101 8 3.02334083506015 1101 9 3.4840156965479245 1101 10 3.4953126459780393 1101 11 3.3337402998563164 1101 12 3.546304722644166 1101 13 3.518032962271839 1101 14 3.918039537946754 1101 15 3.9488374682548084 1101 16 4.053838677943453 1101 17 3.9821445107898086 1101 18 3.9364333773643025 1101 19 3.9545633139373706 1101 20 4.07342556235281 1101 21 3.9524844002278954 1101 22 3.9973502276711415 1101 23 4.043804785825344 1101 24 4.17079759238856 1101 25 4.058562356637957 1101 26 4.216472611727195 1101 27 4.155376043646228 1101 28 4.172326724475269 1101 29 4.248982848558708 1101 30 4.437023316001103 1101 31 4.361427396950834 1101 32 4.416119584570023 1101 33 4.48927873916459 1101 34 4.490001201616234 1101 35 4.363401771516084 1101 36 4.441617782330276 1101 37 4.3976882048004775 1101 38 4.434348909211501 1101 39 4.5941791178025575 1101 40 4.476846884226024 1101 41 4.57941851587967 1101 42 4.5841331104962535 1101 43 4.524445885689008 1101 44 4.4713602641026 1101 45 4.5929407974315275 1101 46 4.578260206454853 1101 47 4.5906849309319 1101 48 4.550708504146904 1101 49 4.648727257196285 1101 50 4.732696499933063 1102 1 0.857725165512619 1102 2 0.8791536448303674 1102 3 1.08285966181095 1102 4 1.2626010032825796 1102 5 1.3319782744123572 1102 6 1.2509614516285588 1102 7 1.3551256378611214 1102 8 1.3596895501611037 1102 9 1.3732970418483283 1102 10 1.5681859401948515 1102 11 1.3327501586532833 1102 12 1.4883246238782704 1102 13 1.474400630092255 1102 14 1.7964349314781813 1102 15 1.5200584403131643 1102 16 1.631796234659376 1102 17 1.9453786952748047 1102 18 1.875489444947398 1102 19 1.9166170172631638 1102 20 1.8856690894213912 1102 21 1.7840101446680023 1102 22 1.7980071805931388 1102 23 1.7750346631137361 1102 24 1.5744097900495149 1102 25 1.8027831281419253 1102 26 1.788730513691025 1102 27 1.6512614934800638 1102 28 1.7920550600429621 1102 29 1.711518497486157 1102 30 1.8442793211553266 1102 31 1.8824535368982573 1102 32 1.8709341091012526 1102 33 1.8895709159397711 1102 34 1.7801014810084972 1102 35 1.8948833193039327 1102 36 1.9009914595334862 1102 37 1.9397589365779773 1102 38 1.7888325299805032 1102 39 2.0237392754431447 1102 40 1.9342753156336734 1102 41 1.8020178545554053 1102 42 1.9372241632881853 1102 43 1.7149510301397903 1102 44 1.624098029241324 1102 45 1.6230766757773836 1102 46 1.664136476477065 1102 47 1.6515610966131078 1102 48 1.5447605178074504 1102 49 1.4831573059993204 1102 50 1.5335310522255874 1103 1 0.0890748438533926 1103 2 0.197889128360071 1103 3 0.04765775123310334 1103 4 0.13112618695569012 1103 5 0.2700113492757801 1103 6 0.26381779601461375 1103 7 0.0796432769955473 1103 8 0.22082061881753745 1103 9 0.23427560724913477 1103 10 0.24062585044615048 1103 11 0.34567571605535363 1103 12 0.2830089042799244 1103 13 0.31236410801538383 1103 14 0.25704963264272607 1103 15 0.32169718910819967 1103 16 0.2286329225488828 1103 17 0.37126376604858535 1103 18 0.11800886698352286 1103 19 0.1917728291995644 1103 20 0.19791283996061537 1103 21 0.1650426748921774 1103 22 0.1467493326742897 1103 23 0.28161853793138647 1103 24 0.22038499984547144 1103 25 0.26460132527829217 1103 26 0.26956741316546484 1103 27 0.10867730555083455 1103 28 0.2835233027330233 1103 29 0.2726583857364595 1103 30 0.4183692419805425 1103 31 0.3030839640278806 1103 32 0.35021894135140386 1103 33 0.3748428873664231 1103 34 0.41541484693771125 1103 35 0.414356438685509 1103 36 0.41611002441599254 1103 37 0.4003549214338733 1103 38 0.3845009149571581 1103 39 0.3700277247932391 1103 40 0.3031654939867643 1103 41 0.24614519413398028 1103 42 0.402547962359705 1103 43 0.3423677668282039 1103 44 0.33857380889822153 1103 45 0.27777352543338196 1103 46 0.34170070940861663 1103 47 0.31632566731271083 1103 48 0.25724932934744515 1103 49 0.22501157922052784 1103 50 0.2123647414531753 1104 1 0.16937889770952097 1104 2 0.20283683107879114 1104 3 0.11412480387829405 1104 4 0.20485104498532536 1104 5 0.19340125143216014 1104 6 0.06238720883122184 1104 7 0.17773408485097875 1104 8 0.2556201567970042 1104 9 0.34230197468705015 1104 10 0.19558583375356164 1104 11 0.26138842509012505 1104 12 0.27782983076359 1104 13 0.2598022870812393 1104 14 0.3862832923618029 1104 15 0.35356520410088166 1104 16 0.3628826506859397 1104 17 0.33790238640443704 1104 18 0.1941492379429306 1104 19 0.292381766133218 1104 20 0.2630511103760256 1104 21 0.22814673902343685 1104 22 0.25690098555139684 1104 23 0.2429426152310441 1104 24 0.3344164624883703 1104 25 0.3980107456840827 1104 26 0.2724537345725828 1104 27 0.417203459148773 1104 28 0.17678470711860683 1104 29 0.21906391375896206 1104 30 0.2532759928792778 1104 31 0.2686399997907607 1104 32 0.41952296771703956 1104 33 0.3272876709103443 1104 34 0.24489245097457513 1104 35 0.23189708518076202 1104 36 0.3771573490728457 1104 37 0.415305242509298 1104 38 0.35109829957483285 1104 39 0.3688664092334868 1104 40 0.3458662951318132 1104 41 0.3671877237279446 1104 42 0.3106154303366644 1104 43 0.46076129457489756 1104 44 0.46086634468881726 1104 45 0.3878620853777548 1104 46 0.39193751070508753 1104 47 0.2672063770541264 1104 48 0.3100956092754643 1104 49 0.4209113948539631 1104 50 0.3386164038392856 1105 1 0.15483450460776282 1105 2 0.19510926657191519 1105 3 0.1031369046886752 1105 4 0.14639410388523544 1105 5 0.09022413753592673 1105 6 0.05428065178235997 1105 7 0.13994418440163514 1105 8 0.04052103020626063 1105 9 -0.06229635047847282 1105 10 -0.2195804119298721 1105 11 -0.04211231563084539 1105 12 0.06353492781073555 1105 13 0.0721882782631944 1105 14 0.15852517768933438 1105 15 -0.036223006962181614 1105 16 -0.08422883890586519 1105 17 -0.009346957795962396 1105 18 -0.03458277088975592 1105 19 -0.1425292031241696 1105 20 -0.04617072575953074 1105 21 0.030927424051483854 1105 22 -0.021002711681653352 1105 23 -0.09085337630824597 1105 24 0.0827646396603896 1105 25 -0.1099813774017413 1105 26 -0.06740105742496283 1105 27 0.07357182973915855 1105 28 0.02071258186293079 1105 29 0.004333274861835434 1105 30 0.028482785555333123 1105 31 -0.06415953799591279 1105 32 0.054120298366182595 1105 33 0.12252850101723922 1105 34 0.13495404641133746 1105 35 0.12132300813286534 1105 36 0.01880704940482758 1105 37 0.05168788075030761 1105 38 0.02135804056143442 1105 39 0.07607678216852065 1105 40 0.08167766766344187 1105 41 0.07946225158021392 1105 42 0.09226259917239332 1105 43 0.1750074188670574 1105 44 0.15849130179760706 1105 45 0.21777042196514884 1105 46 0.2486220169578388 1105 47 0.1305936654126434 1105 48 0.14565465018280932 1105 49 0.18822723818878728 1105 50 0.12701130175497966 1106 1 -0.3725055231409513 1106 2 -0.45703165124878 1106 3 -0.5035332315023844 1106 4 -0.6203324464617275 1106 5 -0.5265776112562932 1106 6 -0.5059254514327414 1106 7 -0.511805138691507 1106 8 -0.5032633433125255 1106 9 -0.446139936676488 1106 10 -0.5124153203749131 1106 11 -0.5778637100801163 1106 12 -0.5155051424337529 1106 13 -0.5427609810215444 1106 14 -0.4640171262377244 1106 15 -0.5467863535057802 1106 16 -0.5536116384840319 1106 17 -0.45557344430862656 1106 18 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4 -0.4951539305957269 1107 5 -0.500430186176196 1107 6 -0.5151691031651637 1107 7 -0.45731004769507066 1107 8 -0.4421944050130122 1107 9 -0.43754657892011295 1107 10 -0.587027384306424 1107 11 -0.577609793137146 1107 12 -0.7420011195809693 1107 13 -0.5756052773460993 1107 14 -0.7355732341850647 1107 15 -0.5800000656571065 1107 16 -0.5207120007360011 1107 17 -0.58685211585302 1107 18 -0.541206640491616 1107 19 -0.5299090943248927 1107 20 -0.49296548232891624 1107 21 -0.5363359764084983 1107 22 -0.8277435563648723 1107 23 -0.8307900914078381 1107 24 -0.7747087531558847 1107 25 -0.7460734171272243 1107 26 -0.3488849884016503 1107 27 -0.5072387825049027 1107 28 -0.5803739101159451 1107 29 -0.445947114015933 1107 30 -0.5566109155949093 1107 31 -0.5732267695901996 1107 32 -0.6999567752341491 1107 33 -0.6272342744045893 1107 34 -0.5377267720970202 1107 35 -0.49863145020548316 1107 36 -0.6849533438797586 1107 37 -0.5599352367592552 1107 38 -0.5333100309204184 1107 39 -0.5150791922241886 1107 40 -0.4820903795672713 1107 41 -0.4761804701275558 1107 42 -0.6103153378844723 1107 43 -0.7130433864174393 1107 44 -0.47601716580665965 1107 45 -0.4153721709830549 1107 46 -0.5858249018683779 1107 47 -0.45589490261384924 1107 48 -0.5446507947949009 1107 49 -0.5071195275243987 1107 50 -0.4998807390619753 1108 1 -0.4221580956270685 1108 2 -0.31792401424592853 1108 3 -0.32446711775824966 1108 4 -0.5458695938665521 1108 5 -0.5730366810827725 1108 6 -0.6752449881937643 1108 7 -0.5975503418076293 1108 8 -0.42819084153318343 1108 9 -0.6119329316198368 1108 10 -0.635320115289133 1108 11 -0.5926235970433389 1108 12 -0.5898873635925241 1108 13 -0.5633142731657586 1108 14 -0.6649657707499017 1108 15 -0.4892301837762183 1108 16 -0.5959621954760372 1108 17 -0.750463333692938 1108 18 -0.6429502971550143 1108 19 -0.7082529850493664 1108 20 -0.8126334119939358 1108 21 -0.7326145172265868 1108 22 -0.8155612403539365 1108 23 -1.0348732397225966 1108 24 -0.8507892848156793 1108 25 -0.6083236250929644 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18 0.4025869226974237 2215 19 0.26845154077953737 2215 20 0.2925569073409733 2215 21 0.3570287537368415 2215 22 0.2629102656882907 2215 23 0.17724383045464265 2215 24 0.1649258487261278 2215 25 0.23670862151089977 2215 26 0.11031914421834088 2215 27 0.08672073997107077 2215 28 0.16902765651814633 2215 29 0.1467948192928894 2215 30 0.12777740396392545 2215 31 0.1744998309145096 2215 32 0.15495282370356733 2215 33 0.14221101162345562 2215 34 0.17735057517952768 2215 35 0.27314342413384907 2215 36 0.22042720723813147 2215 37 0.2451235547226993 2215 38 0.22799951911055416 2215 39 0.18298224867281832 2215 40 0.16082753711325282 2215 41 0.19622758474149649 2215 42 0.341930956125978 2215 43 0.16378488527751145 2215 44 0.16600537085317413 2215 45 0.24480249981791147 2215 46 0.1974815357516988 2215 47 0.12428069417880394 2215 48 0.15627070178747005 2215 49 0.05464919751091202 2215 50 0.1079075252969659 2216 1 0.4591061037826947 2216 2 0.44843803845710184 2216 3 0.4100166437444557 2216 4 0.5819995459666956 2216 5 0.5004711670013471 2216 6 0.4784537557342894 2216 7 0.6389727562309204 2216 8 0.46633815887816965 2216 9 0.7271404576031024 2216 10 0.6608918482209347 2216 11 0.5178361326586653 2216 12 0.3968208434546696 2216 13 0.5225569841910414 2216 14 0.5816689504610935 2216 15 0.46781287286803064 2216 16 0.5093503384506519 2216 17 0.49249241444725556 2216 18 0.5430856011440923 2216 19 0.40456968579922403 2216 20 0.39610939056741046 2216 21 0.272020528684628 2216 22 0.33403940824970135 2216 23 0.31250620037867805 2216 24 0.22549365457793158 2216 25 -0.03217295622046379 2216 26 0.2346579054700264 2216 27 0.16815799853344515 2216 28 0.20233819382661267 2216 29 0.052589322370351965 2216 30 0.15062086920614703 2216 31 0.2062725607076814 2216 32 0.2508717191437455 2216 33 0.31821973572378326 2216 34 0.14343563515771862 2216 35 0.10813224175948832 2216 36 -0.0505448275843392 2216 37 0.07747665898678946 2216 38 -0.04819218929748459 2216 39 -0.01685694693555817 2216 40 -0.1353870905414034 2216 41 -0.06540116042975086 2216 42 -0.014821904159031618 2216 43 -0.06436512488828328 2216 44 -0.24472231522707943 2216 45 -0.37930337231487243 2216 46 -0.3467174920286453 2216 47 -0.2788448256629792 2216 48 -0.4120415914310236 2216 49 -0.4363753297796258 2216 50 -0.5508895839206396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 247407.0 3 11548.0 4 20512.0 5 15736.0 6 12478.0 7 10623.0 8 9346.0 9 8523.0 10 8308.0 11 8326.0 12 8303.0 13 8541.0 14 9100.0 15 9785.0 16 10313.0 17 10955.0 18 12018.0 19 13800.0 20 17608.0 21 23143.0 22 27994.0 23 30685.0 24 32507.0 25 34694.0 26 38577.0 27 42959.0 28 48205.0 29 54593.0 30 63132.0 31 74439.0 32 89670.0 33 108827.0 34 134591.0 35 169402.0 36 216263.0 37 286736.0 38 431133.0 39 551433.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.798297545917386 17.121767945775517 13.135009255306937 24.944925253000154 2 15.804490438175531 19.57212906383555 38.547430347750826 26.075950150238093 3 19.418127169442155 23.79939487645566 28.006488475359536 28.77598947874265 4 13.043584374084775 16.15789813812856 36.122794690895795 34.67572279689088 5 14.605260896614066 37.08138132048299 33.70032893493138 14.613028847971568 6 33.40908122638748 35.448158618424394 17.012525545158066 14.130234610030056 7 29.27144427239423 31.556192450549688 20.77430153893513 18.398061738120948 8 26.97286163160056 34.2844675488884 19.568830303165658 19.173840516345383 9 26.172911957987992 16.764362085564695 18.39392854467727 38.668797411770036 10 14.81291389989072 29.638057782546095 32.54439638336049 23.004631934202692 11 34.99263086653451 23.246266477485495 22.702840122440985 19.058262533539004 12 23.981685753169277 24.84582657194365 29.05469501852074 22.117792656366333 13 28.118157828466316 21.739523285843315 25.177576521523736 24.964742364166632 14 22.18071616106993 21.090904343512147 26.348393471504654 30.379986023913265 15 23.624420797455443 28.79626534390726 22.921596065253414 24.657717793383892 16 23.48838335894653 27.884164448058808 24.560564472933326 24.066887720061334 17 23.136184166223458 27.783217686598032 25.959050198791445 23.12154794838706 18 23.08766379562992 27.408889620324317 26.745100518353322 22.758346065692443 19 23.85195070796144 26.763753088998904 26.968059957503073 22.416236245536584 20 24.13543089116886 26.43590873135595 26.24726047685056 23.181399900624637 21 24.40552838923478 26.1600500093991 26.24232220665443 23.19209939471169 22 23.776721494351985 26.21817503229622 26.38894378034376 23.616159693008036 23 23.921179767810777 26.469358395943477 26.143912789373264 23.465549046872482 24 23.44831164939409 26.403247384542 26.684286907743914 23.46415405832 25 23.79360829773414 26.18247179272161 26.2694220386224 23.754497870921846 26 23.47225210203017 26.346567434392533 26.53300598046743 23.64817448310987 27 23.540418582929462 26.494272603653453 26.328565915488216 23.636742897928873 28 22.745086716725822 26.525817723360273 26.795567956916955 23.93352760299695 29 23.332826989022408 26.79075768125465 26.221635840379193 23.654779489343746 30 23.595811827083047 26.535347804137245 26.349700135369524 23.519140233410187 31 23.662766987072338 26.41050017161522 26.581565673657597 23.345167167654843 32 23.71275802987706 26.497761161468137 26.033502355650484 23.755978453004317 33 23.262865206042914 26.75560188457089 26.116829514911615 23.864703394474578 34 23.639160194329857 26.503502053271337 26.185711375291554 23.671626377107252 35 23.784272226268847 26.4686035485068 26.4770556464137 23.27006857881066 36 23.87355521342866 26.653406863511407 25.99896033431471 23.474077588745224 37 23.789850979292677 26.094291364825178 26.37146328356814 23.744394372314005 38 23.859043259490637 26.30495300674709 26.088306844623887 23.747696889138386 39 23.75424956065684 26.425455963471382 26.099062219236963 23.72123225663481 40 23.74685668403228 26.133595094520228 26.67775509776361 23.44179312368388 41 23.59106205744171 26.391452037777803 26.38372251208898 23.63376339269151 42 23.519098171665068 26.578185845450324 26.28485667096292 23.617859311921688 43 23.53577085546866 25.83021850033888 27.04317414449871 23.590836499693754 44 23.855290986843166 26.24485978033816 26.36814604458573 23.531703188232946 45 23.55711053551206 26.458260271907008 26.451253244614293 23.53337594796664 46 23.430932163512104 26.66637357021023 26.04740194034434 23.855292325933338 47 23.885906247745844 26.352991285650003 26.030728646064116 23.730373820540034 48 23.544320492841862 26.592742219402695 26.40403841259363 23.458898875161818 49 24.096825847154033 26.03743696448579 25.946314109719747 23.91942307864043 50 23.73243168885572 26.595981824682863 25.918821084274658 23.752765402186768 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2802.0 1 2025.0 2 1248.0 3 1168.0 4 1088.0 5 1045.0 6 1002.0 7 1022.5 8 1043.0 9 1172.5 10 1302.0 11 1600.0 12 1898.0 13 2988.5 14 4079.0 15 5567.5 16 7056.0 17 7845.5 18 8635.0 19 9166.0 20 9697.0 21 10971.5 22 12246.0 23 15679.5 24 19113.0 25 23884.0 26 28655.0 27 34195.5 28 39736.0 29 46766.0 30 53796.0 31 60528.5 32 67261.0 33 75104.5 34 82948.0 35 96138.0 36 109328.0 37 115686.5 38 122045.0 39 134023.5 40 146002.0 41 161165.5 42 176329.0 43 181639.0 44 186949.0 45 194644.5 46 202340.0 47 214571.5 48 226803.0 49 230875.0 50 234947.0 51 227509.0 52 220071.0 53 211086.0 54 202101.0 55 198126.0 56 194151.0 57 176725.5 58 159300.0 59 141308.5 60 123317.0 61 107885.5 62 92454.0 63 78496.0 64 64538.0 65 53864.0 66 43190.0 67 34351.0 68 25512.0 69 20430.0 70 15348.0 71 12338.5 72 9329.0 73 7715.0 74 6101.0 75 4778.5 76 3456.0 77 2740.0 78 2024.0 79 1633.5 80 1243.0 81 981.0 82 719.0 83 577.0 84 435.0 85 324.5 86 214.0 87 175.0 88 136.0 89 106.0 90 76.0 91 61.0 92 46.0 93 35.5 94 25.0 95 22.5 96 20.0 97 20.5 98 21.0 99 29.5 100 38.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08690985171757698 2 0.09721129601440555 3 0.10490304108937086 4 0.10634524329092687 5 0.09693659083315678 6 0.057722426209896045 7 0.05370486293413291 8 0.060366463579415386 9 0.062049032814564045 10 0.04580708897323101 11 0.04886318411462348 12 0.05810014583411309 13 0.05136986889351843 14 0.052434351470857385 15 0.05253736591382568 16 0.05507838884037672 17 0.055799489941154724 18 0.05665794363255709 19 0.06472740833173947 20 0.06987813048015375 21 0.0810380284683847 22 0.08344169880431136 23 0.05981705321691786 24 0.07904641590433117 25 0.08594838358320632 26 0.08265192140822117 27 0.07732950852152641 28 0.09803541155815182 29 0.08471221026758688 30 0.0824115543746285 31 0.056039856974747386 32 0.05329280516225977 33 0.055971180679435184 34 0.05123251630289405 35 0.05813448398176919 36 0.05707000140443024 37 0.05992006765988614 38 0.050683105940396524 39 0.05555912290756205 40 0.05741338288099119 41 0.04453657750995549 42 0.0394545316568534 43 0.03921416462326073 44 0.03784063871701692 45 0.02918742550768093 46 0.026474711842849406 47 0.03169411028657588 48 0.0265090499905055 49 0.026062654070976264 50 0.02719581294362741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2912213.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.71036330720328 #Duplication Level Percentage of deduplicated Percentage of total 1 80.70613601827442 46.57580430735182 2 10.98498314556837 12.678947365085108 3 3.6915330769191694 6.391191450886898 4 1.6093430767232673 3.715030945745283 5 0.8904142224577709 2.5693064135969443 6 0.4812387446227951 1.6663477673810354 7 0.31216539246773006 1.2610624747873882 8 0.20937813225882385 0.9666630464992307 9 0.1554305146311874 0.8072956321552262 >10 0.8013188005415651 8.653759215120772 >50 0.0836708179850818 3.3795207456504683 >100 0.06677447669184276 7.712640752990802 >500 0.005982099245584373 2.301039269202639 >1k 0.00163148161237511 1.3213906135463793 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.433814765609521E-5 0.0 0.0 0.0 3 0.0 3.433814765609521E-5 0.0 0.0 0.0 4 0.0 3.433814765609521E-5 0.0 0.0 0.0 5 0.0 3.433814765609521E-5 0.0 0.0 0.0 6 0.0 3.433814765609521E-5 0.0 0.0 0.0 7 0.0 3.433814765609521E-5 0.0 0.0 0.0 8 0.0 3.433814765609521E-5 0.0 0.0 0.0 9 0.0 3.433814765609521E-5 0.0 3.433814765609521E-5 0.0 10 0.0 3.433814765609521E-5 0.0 3.433814765609521E-5 0.0 11 0.0 3.433814765609521E-5 0.0 3.433814765609521E-5 0.0 12 0.0 3.433814765609521E-5 0.0 3.433814765609521E-5 6.867629531219042E-5 13 0.0 6.867629531219042E-5 0.0 3.433814765609521E-5 6.867629531219042E-5 14 0.0 6.867629531219042E-5 0.0 3.433814765609521E-5 6.867629531219042E-5 15 0.0 6.867629531219042E-5 0.0 3.433814765609521E-5 1.0301444296828563E-4 16 0.0 6.867629531219042E-5 0.0 3.433814765609521E-5 1.0301444296828563E-4 17 0.0 6.867629531219042E-5 0.0 3.433814765609521E-5 1.3735259062438085E-4 18 3.433814765609521E-5 6.867629531219042E-5 0.0 6.867629531219042E-5 2.0602888593657126E-4 19 3.433814765609521E-5 6.867629531219042E-5 0.0 6.867629531219042E-5 2.0602888593657126E-4 20 3.433814765609521E-5 6.867629531219042E-5 0.0 6.867629531219042E-5 2.747051812487617E-4 21 3.433814765609521E-5 6.867629531219042E-5 0.0 6.867629531219042E-5 2.747051812487617E-4 22 3.433814765609521E-5 6.867629531219042E-5 0.0 1.0301444296828563E-4 2.747051812487617E-4 23 3.433814765609521E-5 6.867629531219042E-5 0.0 1.3735259062438085E-4 2.747051812487617E-4 24 3.433814765609521E-5 6.867629531219042E-5 0.0 2.4036703359266647E-4 2.747051812487617E-4 25 3.433814765609521E-5 6.867629531219042E-5 0.0 3.777196242170473E-4 3.090433289048569E-4 26 3.433814765609521E-5 6.867629531219042E-5 0.0 6.180866578097138E-4 3.433814765609521E-4 27 3.433814765609521E-5 6.867629531219042E-5 0.0 7.897773960901898E-4 3.433814765609521E-4 28 3.433814765609521E-5 6.867629531219042E-5 0.0 0.001785583678116951 3.433814765609521E-4 29 3.433814765609521E-5 1.0301444296828563E-4 0.0 0.0032621240273290448 3.433814765609521E-4 30 3.433814765609521E-5 1.0301444296828563E-4 0.0 0.0056314562155996145 3.433814765609521E-4 31 3.433814765609521E-5 1.0301444296828563E-4 0.0 0.012293056860882084 3.777196242170473E-4 32 3.433814765609521E-5 1.0301444296828563E-4 0.0 0.019881787492879128 3.777196242170473E-4 33 3.433814765609521E-5 1.0301444296828563E-4 0.0 0.028741029588151692 3.777196242170473E-4 34 3.433814765609521E-5 1.0301444296828563E-4 0.0 0.03972923683810216 4.4639591952923773E-4 35 3.433814765609521E-5 1.0301444296828563E-4 0.0 0.05507838884037672 4.8073406718533295E-4 36 3.433814765609521E-5 1.0301444296828563E-4 0.0 0.0738270174606047 4.8073406718533295E-4 37 3.433814765609521E-5 1.7169073828047604E-4 0.0 0.09834445488705668 4.8073406718533295E-4 38 3.433814765609521E-5 1.7169073828047604E-4 0.0 0.1254715915353719 5.494103624975234E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4285 0.0 19.15636 1 GGTATCA 2440 0.0 18.218664 1 TAATCCG 80 0.0019852098 16.505028 5 GTATTAG 345 0.0 15.309011 1 GTAGCGA 175 6.434311E-8 15.082793 33 GTACATG 7360 0.0 14.920305 1 ATCATAC 995 0.0 14.819349 3 ATAACGC 135 1.9512689E-5 14.671892 3 ATCAACG 5480 0.0 14.417544 3 TCAACGC 5475 0.0 14.310121 4 TATCAAC 5655 0.0 14.165479 2 TATCATA 780 0.0 14.107104 2 CAACGCA 5560 0.0 14.011463 5 TACATGG 7385 0.0 14.00586 2 AACGCAG 5740 0.0 13.757863 6 TATAACG 130 2.3259265E-4 13.54282 2 TCATACT 900 0.0 13.449234 4 ACATGGG 7440 0.0 13.370354 3 GTCCTAC 430 0.0 13.306378 1 CGAACAT 135 3.215878E-4 13.034064 10 >>END_MODULE