FastQCFastQC Report
Mon 6 Feb 2017
SRR2077104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912213
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.11094655507684363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32050.022.5186181
GTATCAA59800.019.9431631
ATAGGCC8400.015.9780626
GCACCGT2101.5097612E-1015.7161266
AATATCG8750.015.08203933
ATATCGA8700.014.91590634
TATAGGC9350.014.8278135
ATCAACG80150.014.6879483
TCGCACG1057.1483734E-414.67065213
TTACGCA1503.2664266E-614.66133342
TCGAACG1057.18382E-414.6610844
TCAACGC81300.014.4806814
TATCAAC82300.014.4109412
TATCATA7550.014.2807992
CAACGCA82600.014.2801555
GTACATG74350.014.2646781
TACATGG73450.014.1101792
TATCGAA9300.013.95335135
AACGCAG85000.013.8745666
ACATGGG74850.013.5525183
Produced by FastQC (version 0.11.5)