FastQCFastQC Report
Mon 6 Feb 2017
SRR2077103_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077103_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3843085
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27700.020.0976221
GTATCAA50000.019.3196131
TATCAAC64450.014.9195852
TCAACGC63950.014.7603974
ATCAACG64450.014.6809823
GTACATG96650.014.2520181
CAACGCA66850.014.21845155
TACATGG92600.014.1622922
GTCTACG2358.203642E-1014.0451811
AACGCAG67200.014.0099656
GCACCGT3350.013.7891346
TATACTG8000.013.2014385
TATTCCG2054.421563E-712.8794525
ACATGGG96600.012.7789543
TAGTACT5700.012.3525444
CATGGGG67500.012.3216624
GTATAGG6650.012.2428911
ACCGTGC5750.012.2416248
ACGCAGA77850.011.9521037
CTACACG3154.2200554E-1011.8746184
Produced by FastQC (version 0.11.5)