FastQCFastQC Report
Mon 6 Feb 2017
SRR2077103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3843085
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30050.025.1138821
GTATCAA66050.023.3178981
TATCAAC86950.017.4346732
TCAACGC89250.017.0106934
ATCAACG89550.017.0024033
CAACGCA92150.016.4278165
GGACCGT3000.016.1352946
AACGCAG93700.016.1321646
TGGACCG3350.015.7635225
CGTCGAG950.00623430913.89769310
GTACATG89300.013.7728731
TACATGG89400.013.4867482
GTTCGAC1150.001422430313.39432711
ACGCAGA112450.013.3444417
CGCAGAG114850.013.0276098
TCTACAC11450.012.6825993
ACATGGG92350.012.5081263
ACCGTGC5900.012.3069028
GAGTACT74050.012.18418612
AGAGTAC113600.012.18408811
Produced by FastQC (version 0.11.5)