FastQCFastQC Report
Mon 6 Feb 2017
SRR2077102_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077102_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334306
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50800.018.150871
TAGATCG1452.2830009E-615.1758515
GTACATG101350.014.2221321
TATCAAC62850.014.2156892
TCAACGC62850.014.2154754
ATCAACG63650.014.1409493
CAACGCA63150.014.0776155
TACATGG102250.013.7310742
GTATTAG4500.013.6928121
CTTAGAC4350.013.659293
AACGCAG66000.013.5646496
CCTAGAC5100.013.3765833
GTCTTAG7200.013.1426541
ACATGGG107900.012.9306833
GGTATCA30450.012.8641131
ATTATAC4850.012.2511183
TTATACT5750.012.2468034
GGACCGT2706.553819E-912.2204066
GTCCTAA4150.012.1962651
TATACTG5600.012.1813315
Produced by FastQC (version 0.11.5)