FastQCFastQC Report
Mon 6 Feb 2017
SRR2077102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334306
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGT1355.549009E-817.92172243
GTATCAA53500.017.3960551
CACGTAA1105.6351266E-515.99696837
GTACATG98250.014.60088351
TACATGG101300.014.0315862
GGTATCA32700.013.9952191
TAGATCG950.006236443313.8968975
GTATAAT5050.013.506241
TATCAAC69850.013.3562072
ACATGGG106800.013.06195453
ATACCGT2401.6609192E-812.83455856
TCAACGC71850.012.8316944
CAACGCA72400.012.7644165
ATCAACG72350.012.7124113
CGCGATC1751.5988011E-512.57168623
TAACGTC1751.6032425E-512.56829344
GCCGTAC1953.5494177E-612.4114411
AACGCAG75750.012.1991846
TACACCG3255.4569682E-1112.186515
GCACCGT5600.012.1797346
Produced by FastQC (version 0.11.5)