FastQCFastQC Report
Mon 6 Feb 2017
SRR2077101_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077101_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491326
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43290.12399300437713351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1509.598807E-917.602265
TACGGTA850.002977475815.5325194
GTATCAA62750.014.93823051
GCGATAC2950.014.91503114
GTATATA5500.014.4026751
ATACCGA1402.761831E-514.1416326
GTACATG98850.014.0015891
ATCGGCG1100.001019784613.99798441
GTCCTAT7450.013.5864151
TACATGG99150.013.5821422
CGTACAC3900.013.5413643
TATGCGA1302.3310816E-413.53942417
TGCGATA3300.013.3325613
GTGCGAT4300.013.30117312
CCCTATA2502.071829E-913.202642
CGATACT3700.013.08107215
CTATACA5800.012.8991324
CGTGCCG3650.012.65589410
ACATGGG104250.012.4957253
GGTGCGA4050.012.49238711
Produced by FastQC (version 0.11.5)