FastQCFastQC Report
Mon 6 Feb 2017
SRR2077101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491326
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41510.11889465492480507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70900.014.8328681
TATAGCG1057.159462E-414.66778855
TATACTG7200.014.66778855
GTACCGA3250.014.2170846
GTACATG91350.013.8003321
TATACCG1606.3453845E-613.7510525
TAGGACA7250.013.6558274
GTATAAC3600.013.4450881
CTAACGC1150.001424446913.3917543
GTATTAG3800.013.3164261
TACATGG93700.013.3131472
CTGTACG2857.8216544E-1113.1247529
ACCGCGT1353.205953E-413.038978
GGACCGT3051.8189894E-1112.9851596
GTATAAG5800.012.517841
ACTATAC3357.2759576E-1212.478033
ACCGTCG1609.6486634E-512.3768348
ACATGGG98700.012.3712353
GACCGTG5200.012.2708747
TACCGAT3052.4556357E-1012.2639377
Produced by FastQC (version 0.11.5)