FastQCFastQC Report
Mon 6 Feb 2017
SRR2077100_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077100_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118137
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17650.024.9348811
GTATCAA37350.021.6809961
ATCAACG48500.016.561023
TCGATCG1206.4275027E-616.49609641
TATCAAC49200.016.4587842
CAACGCA49000.016.3460715
TCAACGC48950.016.3183384
AACGCAG50850.015.7045756
ACCGTAC1004.9478625E-415.3995688
GTACATG82000.013.9812471
TACATGG81200.013.7122752
ATACCGC1453.8406244E-513.6547896
GCACCGT3900.013.5380816
GCGATAC2309.169526E-913.39071414
GTGTAGC10450.013.26619151
TAAGACG1000.00875132913.2030194
CGCGATC1000.00875504113.20217123
CGTTGCG1000.00876339913.20026416
ACGCAGA60300.013.1702317
GTCTAGG5600.012.9672511
Produced by FastQC (version 0.11.5)