FastQCFastQC Report
Mon 6 Feb 2017
SRR2077100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118137
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.025.6701951
GTATCAA47850.023.0836321
CGCGATC803.6182919E-622.000223
CGCGTTG1408.399002E-817.283137
ATCAACG65350.016.56543
TATCAAC66200.016.5188872
TCAACGC66600.016.1556324
CAACGCA68600.015.6202225
CGTATAC1301.3685416E-515.2328623
AACGCAG70850.015.1855476
GCCGTAC2051.8098945E-915.0264561
GTACCGA3300.014.6679776
GCACCGT4700.014.5119356
CGTGCCG3650.013.86603210
CGTACAC2703.274181E-1113.8537563
CGCACGA2703.274181E-1113.850235
GCGCGTT1751.0557469E-613.82670136
ACCGTGC6700.013.7924998
GTACATG79900.013.7415631
CGAATCG3051.8189894E-1213.70284537
Produced by FastQC (version 0.11.5)