FastQCFastQC Report
Mon 6 Feb 2017
SRR2077099_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077099_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863246
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.022.4436021
GTATCAA32100.022.3491691
TATCAAC42800.016.6587522
TCAACGC42150.016.653734
CAACGCA42300.016.5412085
ATCAACG42750.016.472623
GCACCGT4250.016.0449876
AACGCAG45200.015.5732116
CGTGCCG2700.015.47867110
GTCTACG1554.5578636E-614.1976711
GTACATG74850.014.0241131
CGTTATA950.00623130913.8985292
TACATGG74900.013.7794622
CGCACGA2907.2759576E-1213.65269435
ACCGTGC6700.013.4611848
CGTACAA3151.8189894E-1213.27276725
CGTTAAT2003.2910611E-713.19599141
GTATAGA5600.012.9680511
ATTATAC4100.012.8817893
ACATGGG78900.012.7743053
Produced by FastQC (version 0.11.5)