##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077099_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2863246 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.76082844436 31.0 30.0 34.0 22.0 34.0 2 28.870828423404767 31.0 30.0 34.0 20.0 34.0 3 28.947269637327704 31.0 30.0 34.0 22.0 34.0 4 32.34043809019553 35.0 33.0 37.0 22.0 37.0 5 32.295614138638456 35.0 33.0 37.0 22.0 37.0 6 32.45099827258992 35.0 33.0 37.0 23.0 37.0 7 32.31587296376211 36.0 33.0 37.0 22.0 37.0 8 32.25341413207248 35.0 33.0 37.0 20.0 37.0 9 33.598333849065014 38.0 34.0 39.0 19.0 39.0 10 33.523737743805455 38.0 33.0 39.0 19.0 39.0 11 33.5696524154753 38.0 34.0 39.0 19.0 39.0 12 33.457716521737915 38.0 33.0 39.0 18.0 39.0 13 33.39603128756663 38.0 33.0 39.0 17.0 39.0 14 34.30343009297839 39.0 33.0 41.0 17.0 41.0 15 34.34210193605439 39.0 33.0 41.0 17.0 41.0 16 34.31038932735783 39.0 33.0 41.0 17.0 41.0 17 34.140643172120036 39.0 33.0 41.0 17.0 41.0 18 34.11501736141428 39.0 33.0 41.0 17.0 41.0 19 34.247269707178496 39.0 34.0 41.0 18.0 41.0 20 34.22980386596192 39.0 34.0 41.0 18.0 41.0 21 34.18470889333295 39.0 34.0 41.0 18.0 41.0 22 34.102483335347365 39.0 34.0 41.0 17.0 41.0 23 34.05763912706069 39.0 33.0 41.0 17.0 41.0 24 34.07176051236953 39.0 33.0 41.0 17.0 41.0 25 33.97701874026891 39.0 33.0 41.0 16.0 41.0 26 33.922101349307745 39.0 33.0 41.0 16.0 41.0 27 33.86402425778295 39.0 33.0 41.0 16.0 41.0 28 33.75081987366786 39.0 33.0 41.0 13.0 41.0 29 33.73458061235395 39.0 33.0 41.0 13.0 41.0 30 33.56324220831881 38.0 33.0 40.0 10.0 41.0 31 33.561935998513576 38.0 33.0 40.0 10.0 41.0 32 33.42898933587963 38.0 33.0 40.0 9.0 41.0 33 33.3930598348867 38.0 33.0 40.0 9.0 41.0 34 33.31577063235223 38.0 33.0 40.0 9.0 41.0 35 33.2399353740475 38.0 33.0 40.0 8.0 41.0 36 33.15116968643281 38.0 32.0 40.0 8.0 41.0 37 33.06168383715545 38.0 32.0 40.0 7.0 41.0 38 32.97150297250044 38.0 32.0 40.0 2.0 41.0 39 32.85305454019669 38.0 32.0 40.0 2.0 41.0 40 32.781623374310136 38.0 32.0 40.0 2.0 41.0 41 32.671829105847 38.0 32.0 40.0 2.0 41.0 42 32.52587517803221 38.0 32.0 40.0 2.0 41.0 43 32.43385758680882 38.0 31.0 40.0 2.0 41.0 44 32.28204806712382 38.0 31.0 40.0 2.0 41.0 45 32.13436463370594 38.0 31.0 40.0 2.0 41.0 46 31.990972483677616 38.0 31.0 40.0 2.0 41.0 47 31.834061760673027 38.0 31.0 40.0 2.0 41.0 48 31.663300673431483 38.0 31.0 40.0 2.0 41.0 49 31.47427744594771 37.0 31.0 40.0 2.0 41.0 50 31.252868597389117 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.6737326889052966 1101 2 1.959231256809698 1101 3 2.0174776811239425 1101 4 2.3308280127850054 1101 5 2.372680938177986 1101 6 2.561735197554917 1101 7 2.683295027398586 1101 8 2.7936081528200205 1101 9 3.2533340953328036 1101 10 3.0985048839239298 1101 11 3.08425154078067 1101 12 3.2416636888527677 1101 13 3.17480963960179 1101 14 3.6422877348083205 1101 15 3.700855317414657 1101 16 3.56783680447662 1101 17 3.7100841898420924 1101 18 3.6469239715674036 1101 19 3.590677372657197 1101 20 3.6308992212332356 1101 21 3.625105391925217 1101 22 3.671428666279013 1101 23 3.6777764961532426 1101 24 3.7324401891165166 1101 25 3.623364056862968 1101 26 3.6898929831851746 1101 27 3.6390857488067 1101 28 3.680086267804633 1101 29 3.7139999378920834 1101 30 3.830470259766379 1101 31 3.755319284228385 1101 32 3.876273644791574 1101 33 3.879303293048096 1101 34 3.9192564186856558 1101 35 3.750025592584329 1101 36 3.9052090591061415 1101 37 3.8859667741740864 1101 38 3.905730401310535 1101 39 3.8411147832323564 1101 40 3.874375256525333 1101 41 3.893300016882435 1101 42 4.0133739167113305 1101 43 3.914433655066489 1101 44 3.9775635230257507 1101 45 3.97250687602439 1101 46 3.948921217880965 1101 47 3.9410186727608156 1101 48 4.015302991902221 1101 49 4.000664040808829 1101 50 3.979912243889025 1102 1 0.9445442580387073 1102 2 1.0305781382688792 1102 3 1.0797868295499207 1102 4 1.3291826972656438 1102 5 1.3333480187994127 1102 6 1.389431941411516 1102 7 1.5463890595679786 1102 8 1.57766227908035 1102 9 1.7937323811580015 1102 10 1.878341717185478 1102 11 1.6970225413165423 1102 12 1.7801864193767258 1102 13 1.8905179275379993 1102 14 2.1303979088935705 1102 15 1.917197199712838 1102 16 2.1045247694507907 1102 17 2.2566292592310617 1102 18 2.2448417997431847 1102 19 2.1096723479784316 1102 20 2.099320414273542 1102 21 2.1316646159378934 1102 22 2.056046831675843 1102 23 2.026189214204429 1102 24 1.9991939512461556 1102 25 2.1785593566463746 1102 26 2.1065474387019165 1102 27 2.0436636348072668 1102 28 2.275340969838396 1102 29 2.0804464623346206 1102 30 2.29505247991969 1102 31 2.1790274670004948 1102 32 2.2983707290396183 1102 33 2.316771347520003 1102 34 2.374768042024975 1102 35 2.370556891662517 1102 36 2.3228698229222715 1102 37 2.247200039743589 1102 38 2.3610841818438857 1102 39 2.3977723279444945 1102 40 2.4774115219007484 1102 41 2.3801835986788404 1102 42 2.352083796801061 1102 43 2.2295803009890207 1102 44 2.3000388887223053 1102 45 2.2608363868489434 1102 46 2.2889244810610805 1102 47 2.3296864292162276 1102 48 2.2428084105597286 1102 49 2.3330102363815755 1102 50 2.30589224183214 1103 1 0.5749262805257302 1103 2 0.7016219923967313 1103 3 0.5966227299670486 1103 4 0.7285804757780951 1103 5 0.7731150256186226 1103 6 0.6690177056568416 1103 7 0.720621370923034 1103 8 0.8908839762928906 1103 9 0.9116858794771687 1103 10 0.8455383110383181 1103 11 0.9000629707356111 1103 12 0.7862395142440377 1103 13 0.8486166929604124 1103 14 0.9703683201450062 1103 15 0.8776675597124495 1103 16 0.8206884387873927 1103 17 1.0585566975272656 1103 18 0.9547387362294302 1103 19 0.9200656116791563 1103 20 0.9379940900444979 1103 21 0.8337577693841638 1103 22 0.8084875419515214 1103 23 0.9408414564589123 1103 24 0.7972592609155882 1103 25 0.8308455492717286 1103 26 0.8985738885637673 1103 27 0.9223956093126091 1103 28 0.9900767618056676 1103 29 0.99209729268415 1103 30 1.151222725405539 1103 31 0.9263301053231032 1103 32 1.070865892341807 1103 33 1.0228694285386837 1103 34 1.0082832908283166 1103 35 0.9020614606963662 1103 36 1.1270467929606625 1103 37 1.1326147022698336 1103 38 0.9750403464906228 1103 39 1.191547448411491 1103 40 1.1275045625874114 1103 41 0.9927720767206125 1103 42 1.0551552463207656 1103 43 0.872863266683261 1103 44 0.7917415021839957 1103 45 0.8495865200224131 1103 46 0.8115016500764654 1103 47 0.8295085750349109 1103 48 0.7507162944794281 1103 49 0.7825994344147347 1103 50 0.7980581672575795 1104 1 0.4873642553814648 1104 2 0.5645508468813745 1104 3 0.5859122961847874 1104 4 0.5735679040985033 1104 5 0.5585161499857207 1104 6 0.5622268745882693 1104 7 0.555748408496612 1104 8 0.5992176203309896 1104 9 0.6986673725457422 1104 10 0.4149133967645895 1104 11 0.6069027462779744 1104 12 0.6057336959301693 1104 13 0.632167101588017 1104 14 0.7483207554263345 1104 15 0.6332034260013941 1104 16 0.8105637569208213 1104 17 0.8168367986611003 1104 18 0.6411457531405773 1104 19 0.7072487125940299 1104 20 0.6300921787469775 1104 21 0.6705772553406462 1104 22 0.788958651718886 1104 23 0.7432875503125445 1104 24 0.7379727249166592 1104 25 0.7843953003350776 1104 26 0.6495894186046272 1104 27 0.7661074081621209 1104 28 0.6040259820270961 1104 29 0.6783379978099617 1104 30 0.8136181619547642 1104 31 0.8430051440708084 1104 32 0.974476778803961 1104 33 0.8246812327436146 1104 34 0.7762190113232847 1104 35 0.7141218671253142 1104 36 0.7902327076178608 1104 37 0.816237804513392 1104 38 0.8579549514899654 1104 39 0.8964083396754461 1104 40 0.8139998422476822 1104 41 0.9696074662415697 1104 42 0.840293390177294 1104 43 0.9155080488882561 1104 44 0.9413405348640396 1104 45 0.9323180464776257 1104 46 0.9445301085989612 1104 47 0.8828674968826071 1104 48 0.9359444636284557 1104 49 0.9222515309487846 1104 50 1.0070515222430814 1105 1 0.40973936526947696 1105 2 0.49640754017597644 1105 3 0.3310504684127409 1105 4 0.4971030491506738 1105 5 0.4270945495066698 1105 6 0.4543837373333659 1105 7 0.41214357215012143 1105 8 0.5106058403363392 1105 9 0.5326995299181121 1105 10 0.3801212602868702 1105 11 0.4686674521603251 1105 12 0.372352585201277 1105 13 0.5172968649943499 1105 14 0.5368089512244723 1105 15 0.5531852068878891 1105 16 0.40497889789598673 1105 17 0.4959224030133029 1105 18 0.41607345001530405 1105 19 0.3688446050120717 1105 20 0.5734887056488347 1105 21 0.4936027748401912 1105 22 0.520471347765465 1105 23 0.4841308531957509 1105 24 0.5293829862271551 1105 25 0.42368233418695667 1105 26 0.5861951850177505 1105 27 0.6130553881338372 1105 28 0.6326561085416458 1105 29 0.6357823530699349 1105 30 0.6563598200214997 1105 31 0.41594147909864887 1105 32 0.6124152160372063 1105 33 0.6675522082943246 1105 34 0.6392348479373311 1105 35 0.6417295573749016 1105 36 0.6612681393344104 1105 37 0.6683521135248469 1105 38 0.6005335293798595 1105 39 0.6979378533652607 1105 40 0.6937970112777379 1105 41 0.6632740946863223 1105 42 0.6245325829304207 1105 43 0.5481126176813405 1105 44 0.5951206824006334 1105 45 0.6954523710326583 1105 46 0.6568706913557847 1105 47 0.4987232218886568 1105 48 0.5762322746593007 1105 49 0.5228191264080237 1105 50 0.6070419667639655 1106 1 0.328310902041423 1106 2 0.3045243823078181 1106 3 0.2624183393511643 1106 4 0.3265622629657159 1106 5 0.30941043730994977 1106 6 0.16235919745606253 1106 7 0.3535483033980711 1106 8 0.3781624562569661 1106 9 0.40197797673575764 1106 10 0.27007303892303725 1106 11 0.2785856525693191 1106 12 0.2922988095442349 1106 13 0.2780141262228639 1106 14 0.4919113269471609 1106 15 0.384192048767261 1106 16 0.3532166721392187 1106 17 0.5804499093881148 1106 18 0.26066432582635457 1106 19 0.44570844908791685 1106 20 0.26050554525599523 1106 21 0.31284966317365104 1106 22 0.4074566288884398 1106 23 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1107 10 -0.1421259715981762 1107 11 -0.11924947028698796 1107 12 -0.18068949924043665 1107 13 -0.03829620505543829 1107 14 -0.2108469612871815 1107 15 -0.16677364084873858 1107 16 -0.22561360375237882 1107 17 -0.12521417248177613 1107 18 -0.04925406288741385 1107 19 -0.2235479102779223 1107 20 -0.043421236828940835 1107 21 -0.15579244604059994 1107 22 -0.4689859425512495 1107 23 -0.4690726572514734 1107 24 -0.39492867964374767 1107 25 -0.3724661777381115 1107 26 -0.09632192386522576 1107 27 -0.20531307240773344 1107 28 -0.22945084187101372 1107 29 -0.10739910340188175 1107 30 -0.23147525262019997 1107 31 -0.18545451480808595 1107 32 -0.15491420669430056 1107 33 -0.20274085948185672 1107 34 -0.07720445815820653 1107 35 -0.0015144232539796576 1107 36 -0.1338822596833964 1107 37 -0.16252799924599515 1107 38 -0.10958970004968904 1107 39 0.04554147294555122 1107 40 0.03243239312981672 1107 41 0.06246923826451933 1107 42 -0.07570273526069826 1107 43 -0.2138795156429012 1107 44 0.03711304308544783 1107 45 -0.0341374704970292 1107 46 -0.06837542646246675 1107 47 -0.12827129872157883 1107 48 -0.09343214113771126 1107 49 -0.18850684044280897 1107 50 -0.0578410562221876 1108 1 0.05202034513663989 1108 2 -0.014843048706758566 1108 3 0.08727235689963209 1108 4 -0.03160008568598016 1108 5 -0.0932818045022401 1108 6 -0.1846155117621393 1108 7 -0.19732608363896276 1108 8 -0.09617214729016865 1108 9 -0.15904224826756064 1108 10 -0.07479118054042289 1108 11 -0.191107959130548 1108 12 -0.30633980436898156 1108 13 -0.22964237747700622 1108 14 -0.15438932717316334 1108 15 -0.20158071907174957 1108 16 -0.17202088045655728 1108 17 -0.21851674886071493 1108 18 -0.20578377025591976 1108 19 -0.1337570978808884 1108 20 -0.22976896415376302 1108 21 -0.29925887079524927 1108 22 -0.3960947347800925 1108 23 -0.49287954400828937 1108 24 -0.28595181520453394 1108 25 -0.07575174844962618 1108 26 -0.27659867694897855 1108 27 -0.29193951734573886 1108 28 -0.42799788629557156 1108 29 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11 -0.3571953168205937 2216 12 -0.13960813719387488 2216 13 -0.005440387077044306 2216 14 -0.239908190873642 2216 15 -0.21287821070482948 2216 16 -0.1894299581111909 2216 17 -0.17585045253272824 2216 18 -0.1796820022836627 2216 19 -0.35547507940369627 2216 20 -0.5813575873893626 2216 21 -0.44515929891308303 2216 22 -0.37419214100413 2216 23 -0.40955620915418933 2216 24 -0.5703360656166723 2216 25 -0.510783563568971 2216 26 -0.39406024973761333 2216 27 -0.36533741798938024 2216 28 -0.3832925238097502 2216 29 -0.40453458451997903 2216 30 -0.46953001622156165 2216 31 -0.7079812188056422 2216 32 -0.5566120667605432 2216 33 -0.6428571589833609 2216 34 -0.7440433483805151 2216 35 -0.8093177952964865 2216 36 -0.7058383703421356 2216 37 -0.8846375658283421 2216 38 -0.853061654652457 2216 39 -0.7486098175970781 2216 40 -0.8763174732162327 2216 41 -0.7816538011347944 2216 42 -1.0109300416917648 2216 43 -0.947225547659535 2216 44 -1.2189302341269368 2216 45 -1.1476053774723773 2216 46 -1.2471332584595096 2216 47 -1.0761220185337912 2216 48 -1.2907276206806166 2216 49 -1.2145945966938854 2216 50 -1.4505714525831124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 152348.0 3 7317.0 4 13414.0 5 10980.0 6 8936.0 7 7911.0 8 7062.0 9 6479.0 10 6528.0 11 6366.0 12 6455.0 13 6986.0 14 7271.0 15 7697.0 16 8380.0 17 9163.0 18 9885.0 19 11221.0 20 13634.0 21 17467.0 22 21011.0 23 24213.0 24 26185.0 25 28534.0 26 31827.0 27 36129.0 28 41170.0 29 47416.0 30 56174.0 31 67317.0 32 81633.0 33 101501.0 34 127943.0 35 165001.0 36 217463.0 37 296034.0 38 455645.0 39 712550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.6622947003351 16.74764993556176 12.473718741687433 25.116336622415698 2 16.782044841751343 18.717482703761075 38.30142910927909 26.199043345208494 3 20.273075605339148 22.688209258909655 27.548192897447194 29.490522238304003 4 13.590046763736849 15.090709622925418 34.97389817140364 36.34534544193409 5 15.460794019682695 36.323946438908976 32.96473727043149 15.250522270976836 6 35.21731153957035 34.170127635038924 16.33742475997449 14.275136065416232 7 30.804706145454418 30.390524811394265 19.789851806037483 19.014917237113835 8 27.652251642761993 34.25607797475856 18.681953450534028 19.409716931945415 9 26.960255159826097 15.740156046714068 17.937020085763837 39.36256870769599 10 15.398946206202568 28.323014353398275 32.29556540090285 23.982474039496303 11 36.82560747128003 22.178021528009264 21.80138968380256 19.194981316908148 12 24.40910212317178 23.723975787496915 28.751264125532643 23.11565796379866 13 29.06797685849572 20.723365937877702 24.752443419008056 25.456213784618527 14 23.0083180024215 20.209478783939844 25.351090984375574 31.431112229263086 15 24.76471546067689 28.078546285030615 22.23261564007109 24.92412261422141 16 25.099746379672915 26.99267373411728 23.7533711795337 24.154208706676105 17 23.944256992860378 27.089087335975982 25.091796143373905 23.874859527789734 18 23.898344292873773 26.673895374069883 25.632045980449643 23.795714352606705 19 24.878386652166252 26.275227780402638 25.46898020917496 23.377405358256148 20 24.85806559614896 25.710842792423318 25.675929288096523 23.755162323331202 21 25.080373039956882 25.54272336504914 25.597633582823555 23.779270012170425 22 24.99291323528024 25.05834565725505 25.473240341464965 24.475500765999747 23 24.61214013520101 25.35163969227705 25.54743479757414 24.488785374947803 24 24.290573531848082 25.4566228399148 25.40192386601862 24.85087976221849 25 24.631791616565533 25.287219317133193 25.559618019630324 24.521371046670954 26 24.375559252838208 25.791622392483642 25.68557407303842 24.14724428163973 27 24.545522223476578 25.62267990287791 25.426149917113488 24.405647956532025 28 24.61492639370584 25.678041024877324 25.36027074447389 24.346761836942946 29 24.579826131576123 25.740615100358315 25.393835993511132 24.285722774554433 30 24.608275763237046 25.7749287400756 25.455705250427734 24.16109024625962 31 24.687218265810312 25.35915212423211 25.267599432513087 24.686030177444493 32 24.384121297931475 25.79607707752269 25.323165537209203 24.496636087336636 33 24.58974633146106 25.51099565792591 25.31202760812729 24.587230402485737 34 24.808990397281804 25.52952620952948 25.24551888292315 24.415964510265564 35 24.648878073190474 25.56257084989118 25.43872768621553 24.349823390702817 36 24.687163007903653 25.621253556435057 25.27355983162538 24.418023604035902 37 24.665820063516204 25.293538791850246 25.494018797665106 24.546622346968444 38 24.8109857810433 25.407116955758198 25.401491428583405 24.38040583461509 39 24.861642538411274 25.621062342100576 25.173964546664852 24.3433305728233 40 24.7982150532374 25.408689210297343 25.4875581383159 24.305537598149357 41 24.48684102029436 25.471441009952215 25.557672123311892 24.484045846441536 42 24.771862792401517 25.46548934509537 25.370911846574124 24.391736015928988 43 24.61402793456337 25.274012303170757 25.463936867900756 24.64802289436512 44 24.666813406423707 25.568703903626915 25.196858371677827 24.567624318271545 45 24.613183718956005 25.58660876795215 25.156700332230557 24.643507180861285 46 24.68673942456262 25.308561972477015 25.19031088682026 24.8143877161401 47 24.507870474045095 25.47625128082764 25.300350369461782 24.71552787566548 48 24.461540987512258 25.632448078944524 25.36621850248396 24.539792431059258 49 24.6991409416699 25.344054765099543 25.28288559198729 24.67391870124326 50 24.816727259769916 25.441767215585337 25.277821736452665 24.463683788192085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1686.0 1 1231.0 2 776.0 3 715.5 4 655.0 5 630.0 6 605.0 7 601.5 8 598.0 9 640.5 10 683.0 11 886.5 12 1090.0 13 1640.5 14 2191.0 15 3052.5 16 3914.0 17 4392.0 18 4870.0 19 5341.5 20 5813.0 21 6986.0 22 8159.0 23 10876.5 24 13594.0 25 17621.5 26 21649.0 27 27029.0 28 32409.0 29 38177.5 30 43946.0 31 50569.0 32 57192.0 33 65325.0 34 73458.0 35 82794.5 36 92131.0 37 99646.5 38 107162.0 39 116175.5 40 125189.0 41 136485.5 42 147782.0 43 157182.5 44 166583.0 45 176705.5 46 186828.0 47 195791.0 48 204754.0 49 209275.0 50 213796.0 51 217095.0 52 220394.0 53 217995.0 54 215596.0 55 209962.5 56 204329.0 57 193886.0 58 183443.0 59 168304.5 60 153166.0 61 135736.0 62 118306.0 63 102378.5 64 86451.0 65 73885.0 66 61319.0 67 50953.5 68 40588.0 69 32914.0 70 25240.0 71 20086.5 72 14933.0 73 12337.5 74 9742.0 75 7558.5 76 5375.0 77 4131.0 78 2887.0 79 2288.5 80 1690.0 81 1335.0 82 980.0 83 770.5 84 561.0 85 440.0 86 319.0 87 254.5 88 190.0 89 145.5 90 101.0 91 72.0 92 43.0 93 32.5 94 22.0 95 20.0 96 18.0 97 16.0 98 14.0 99 20.0 100 26.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08266841200511588 2 0.09265707522161909 3 0.10009618454020366 4 0.10163290195812724 5 0.0929714037843762 6 0.054867796899043954 7 0.05085137637492552 8 0.055950484170762836 9 0.05822063490178629 10 0.04212002740945067 11 0.045403014620469216 12 0.05532182704524864 13 0.04802241931011167 14 0.048231971685283065 15 0.04861615103976396 16 0.051968989042506304 17 0.05134033191699211 18 0.05266749695974429 19 0.060001830090743155 20 0.06597407278312796 21 0.07725497564652146 22 0.07959497716926874 23 0.055217050857662944 24 0.07446094397756951 25 0.08263348660925397 26 0.07983945494030202 27 0.07351795828929823 28 0.09391438947264748 29 0.0819699040878779 30 0.07826781212651655 31 0.0525976461680205 32 0.04889555420665916 33 0.05168958587561111 34 0.04707943362184039 35 0.05455346833628685 36 0.053505706460429875 37 0.05636958892110563 38 0.04512361145357402 39 0.05085137637492552 40 0.05364540804387747 41 0.0408627131584223 42 0.03642718788396107 43 0.03691614342602766 44 0.03618271011292778 45 0.027102107188833935 46 0.024203299332296282 47 0.029302407128133593 48 0.02371434379022969 49 0.024203299332296282 50 0.02476210566608667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2863246.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.886453473094086 #Duplication Level Percentage of deduplicated Percentage of total 1 79.51921018874401 49.21161901562894 2 11.637481541447455 14.404049199175583 3 3.7858234902778314 7.02873567865277 4 1.6926988682124011 4.1902051900634305 5 0.8896328672745033 2.752811152435942 6 0.5486923159941055 2.0373972890888066 7 0.35580604632164364 1.5413702031790968 8 0.26827758709905086 1.3282198729503483 9 0.207376923889423 1.1550440116508534 >10 1.0126677511558113 11.12852570531886 >50 0.05651911388135554 2.3829828444147534 >100 0.024789336706232963 2.3749615982202665 >500 9.67081829723884E-4 0.4238736155601065 >1k 5.688716643847028E-5 0.04020462366030048 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.4925395861899394E-5 2 0.0 0.0 0.0 0.0 3.4925395861899394E-5 3 0.0 0.0 0.0 0.0 3.4925395861899394E-5 4 0.0 0.0 0.0 0.0 3.4925395861899394E-5 5 0.0 0.0 0.0 0.0 3.4925395861899394E-5 6 0.0 0.0 0.0 0.0 6.985079172379879E-5 7 0.0 0.0 0.0 0.0 6.985079172379879E-5 8 0.0 0.0 0.0 0.0 6.985079172379879E-5 9 0.0 0.0 0.0 0.0 6.985079172379879E-5 10 0.0 0.0 0.0 0.0 6.985079172379879E-5 11 0.0 0.0 0.0 0.0 6.985079172379879E-5 12 0.0 0.0 0.0 0.0 6.985079172379879E-5 13 0.0 0.0 0.0 0.0 6.985079172379879E-5 14 0.0 0.0 0.0 0.0 6.985079172379879E-5 15 0.0 3.4925395861899394E-5 0.0 3.4925395861899394E-5 6.985079172379879E-5 16 0.0 3.4925395861899394E-5 0.0 3.4925395861899394E-5 1.047761875856982E-4 17 0.0 3.4925395861899394E-5 0.0 3.4925395861899394E-5 1.047761875856982E-4 18 0.0 3.4925395861899394E-5 0.0 3.4925395861899394E-5 1.3970158344759758E-4 19 0.0 3.4925395861899394E-5 0.0 3.4925395861899394E-5 1.3970158344759758E-4 20 0.0 3.4925395861899394E-5 0.0 3.4925395861899394E-5 2.095523751713964E-4 21 0.0 3.4925395861899394E-5 0.0 6.985079172379879E-5 2.095523751713964E-4 22 0.0 3.4925395861899394E-5 0.0 1.047761875856982E-4 2.444777710332958E-4 23 0.0 3.4925395861899394E-5 0.0 1.047761875856982E-4 2.7940316689519515E-4 24 0.0 3.4925395861899394E-5 0.0 2.095523751713964E-4 2.7940316689519515E-4 25 0.0 3.4925395861899394E-5 0.0 2.7940316689519515E-4 2.7940316689519515E-4 26 0.0 3.4925395861899394E-5 0.0 5.588063337903903E-4 2.7940316689519515E-4 27 0.0 3.4925395861899394E-5 0.0 9.080602924093843E-4 2.7940316689519515E-4 28 0.0 3.4925395861899394E-5 0.0 0.0019558221682663664 3.1432856275709456E-4 29 0.0 6.985079172379879E-5 0.0 0.003771942753085135 3.1432856275709456E-4 30 0.0 6.985079172379879E-5 0.0 0.006496123630313288 3.1432856275709456E-4 31 0.0 6.985079172379879E-5 0.0 0.01393523294889786 3.1432856275709456E-4 32 0.0 6.985079172379879E-5 0.0 0.02273643270609651 3.1432856275709456E-4 33 0.0 6.985079172379879E-5 0.0 0.03405226096535191 3.1432856275709456E-4 34 0.0 6.985079172379879E-5 0.0 0.049419435144587645 3.1432856275709456E-4 35 0.0 6.985079172379879E-5 0.0 0.06817437272242763 3.1432856275709456E-4 36 0.0 6.985079172379879E-5 0.0 0.08958364038577195 3.1432856275709456E-4 37 0.0 1.047761875856982E-4 0.0 0.11686037455391537 3.1432856275709456E-4 38 0.0 1.047761875856982E-4 0.0 0.14686128959928696 3.1432856275709456E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1510 0.0 22.443602 1 GTATCAA 3210 0.0 22.349169 1 TATCAAC 4280 0.0 16.658752 2 TCAACGC 4215 0.0 16.65373 4 CAACGCA 4230 0.0 16.541208 5 ATCAACG 4275 0.0 16.47262 3 GCACCGT 425 0.0 16.044987 6 AACGCAG 4520 0.0 15.573211 6 CGTGCCG 270 0.0 15.478671 10 GTCTACG 155 4.5578636E-6 14.197671 1 GTACATG 7485 0.0 14.024113 1 CGTTATA 95 0.006231309 13.898529 2 TACATGG 7490 0.0 13.779462 2 CGCACGA 290 7.2759576E-12 13.652694 35 ACCGTGC 670 0.0 13.461184 8 CGTACAA 315 1.8189894E-12 13.272767 25 CGTTAAT 200 3.2910611E-7 13.195991 41 GTATAGA 560 0.0 12.968051 1 ATTATAC 410 0.0 12.881789 3 ACATGGG 7890 0.0 12.774305 3 >>END_MODULE