FastQCFastQC Report
Mon 6 Feb 2017
SRR2077099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863246
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21000.022.8383661
GTATCAA38200.020.5604531
CGTGCCG3250.020.3125510
GCACCGT5050.017.863166
CAACGCG1154.2679876E-617.22120714
TATCTCG2850.016.979136
ACCGTGC6900.016.5816468
CTCGTAT2750.015.99460239
ACCGTCG1704.490539E-815.5312258
TACCGTC1901.0766598E-815.0541277
TATCAAC52500.014.8774072
TATGCGC1057.1500917E-414.67017410
CGTCGTA1503.2460084E-614.67017410
TCGTATG3450.014.66171740
AGCACCG5500.014.4024365
ATCAACG53850.014.3818633
TCAACGC53750.014.2860694
CGCACGA2950.014.1666935
TACACCG2652.3646862E-1114.1155955
GTATAGG3900.014.1027321
Produced by FastQC (version 0.11.5)