FastQCFastQC Report
Mon 6 Feb 2017
SRR2077097_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077097_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329034
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT145640.6253236320294165No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT77010.330652107268507No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT72640.31188896340714645No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57430.24658291806817761No Hit
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC47780.20514943105167205No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT46260.19862312014337274No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG45420.19501647464141786No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC39970.17161621513468672No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38820.1666785456974866No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT33180.14246249732721808No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33100.14211900727941282No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC32820.14091679211209454No Hit
CTACAAGAATGACTTCAAGAAAATGGTCACTACTGAGTGTCCTCAGTTTG32680.1403156845284354No Hit
ATATCATACTTGGCATCTTCATAAATGTTCCTGATGTGAGGGGGCACATC31020.13318826603647693No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29400.12623259256842106No Hit
CTCTAGAGTAGCTCTAGCTCCTAAAACATCCATACCTTTGTCCATTTGCT28440.12211071199475834No Hit
CTGCTACTCCTTGTGGCTGTCTTTGTGAGATGCCACACCCACTTTTATCA24910.1069542136353527No Hit
GCCTAAAGACTGCGACTTCCTGGAGGATGGGGAGGAGAGAAATTGCACAG24240.10407748448498391No Hit
CTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCTGCTACTCC23890.10257471552583602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTT1750.020.11307739
TCTTATA14950.019.7255082
ATAGGCC13250.019.5904356
CTTATAG14800.019.1819423
CTCTTAT14300.018.9288541
TATAGGC14000.018.70375
TATCATA8500.018.3824772
CGTTTCA1801.4551915E-1118.33026341
TCATACT8950.017.704114
TTATAGG15100.017.63494
GGTACGT1002.4858977E-517.59894215
GGAACGT1903.274181E-1117.36625937
TAGGCCT15050.017.2470237
CGACGGT1053.7807975E-516.7598197
GTATAAA2007.2759576E-1116.5050371
ACGTTTC2151.2732926E-1116.37110940
CATACTT9900.016.225435
GTACATG92400.016.0525171
CCGCGAT1105.615322E-516.00316422
ATCATAC13800.015.7877663
Produced by FastQC (version 0.11.5)