FastQCFastQC Report
Mon 6 Feb 2017
SRR2077097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329034
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT160250.6880535020098462No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT83630.3590759087243896No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT71180.3056202700347011No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC61100.2623405240112424No Hit
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC53840.23116880217291808No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT49890.21420898106253491No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT45020.19329902440239172No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC44510.1911092753476334No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC43170.18535581704689583No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC41650.17882950613859652No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG40800.17517992438066599No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT37040.15903589213382027No Hit
CTACAAGAATGACTTCAAGAAAATGGTCACTACTGAGTGTCCTCAGTTTG36100.15499988407210885No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC34180.1467561229247834No Hit
ATATCATACTTGGCATCTTCATAAATGTTCCTGATGTGAGGGGGCACATC33200.14254836983916938No Hit
CTCTAGAGTAGCTCTAGCTCCTAAAACATCCATACCTTTGTCCATTTGCT33140.14229075230331545No Hit
CTGCTACTCCTTGTGGCTGTCTTTGTGAGATGCCACACCCACTTTTATCA31700.13610793144282135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30750.13202898712513428No Hit
CTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCCCA27790.11931985535634088No Hit
TTTATATTCTGCACAAACTGAGGACACTCAGTAGTGACCATTTTCTTGAA26820.11515503852670247No Hit
ACTCAGTAGTGACCATTTTCTTGAAGTCATTCTTGTAGAGGGCATGGTGA26470.11365226956755461No Hit
CTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCTGCTACTCC25640.11008856032157538No Hit
TTCTTGTAGAGGGCATGGTGATTTCCTTGTATATTGGAATAATTGTGGTA25220.10828523757059794No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25180.10811349254669532No Hit
GCCTAAAGACTGCGACTTCCTGGAGGATGGGGAGGAGAGAAATTGCACAG24370.1046356558126674No Hit
GAATATAAATATCGAAAACTTGTTCAGAGAATTGGACATCAATAGTGACA23300.10004147642327249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT1100.028.00150325
CGCGAAT508.344077E-526.40028428
CCGCGAT1254.0017767E-1122.88172122
CGCGATC1254.0017767E-1122.8812323
TTCCGCG703.21567E-522.0011825
TATCATA7500.020.8277852
TCATACT9100.020.0683044
TACACCG1352.5975169E-919.5578655
TCTTATA14750.019.5400312
TATAGGC13400.019.3754225
ATAGGCC13450.019.1393976
GCGATCG1504.656613E-1019.0676924
CGACGGT708.117614E-418.860187
CTTATAG14800.018.4334223
TAGGCCT14200.017.9747267
CTCTTAT15550.017.9688111
TTATAGG14450.017.967524
CCGATCG1509.589712E-917.60283518
TACGACG750.001290335617.602085
TTTCTAG7150.017.22873531
Produced by FastQC (version 0.11.5)