FastQCFastQC Report
Mon 6 Feb 2017
SRR2077096_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077096_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584687
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44200.018.8218671
CGTGCCG2850.018.52318610
TACACCG2400.017.4201325
GCGCACT3600.017.10424642
GTGCCGA3350.017.07240311
CGCACGA2109.094947E-1216.75777435
TGCGATA2950.016.4052813
CGACAGG4300.016.3680634
TTGCGCG1906.166374E-1016.21249618
GCACCGT5000.015.8388576
GCGATAC3100.015.61268314
CGCACTC4300.015.34178943
TACCGAT2750.015.1989037
AGCACCG4700.014.9817045
GTACATG67650.014.8350921
TATCAAC54900.014.8319192
CAACGCA54350.014.656095
TATACTG5000.014.5228895
ATCAACG55250.014.4989663
TCAACGC55100.014.4180574
Produced by FastQC (version 0.11.5)