##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077096_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2584687 Sequences flagged as poor quality 0 Sequence length 50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.273026869404305 31.0 29.0 34.0 16.0 34.0 2 28.380162472283878 31.0 28.0 34.0 16.0 34.0 3 28.425330417183975 31.0 28.0 34.0 16.0 34.0 4 31.75823494295441 35.0 32.0 37.0 17.0 37.0 5 31.73411945044023 35.0 32.0 37.0 17.0 37.0 6 31.749660597201906 35.0 32.0 37.0 17.0 37.0 7 31.59300681281718 35.0 32.0 37.0 15.0 37.0 8 31.52402747411969 35.0 32.0 37.0 13.0 37.0 9 32.751711135623 38.0 33.0 39.0 11.0 39.0 10 32.707060468056675 37.0 32.0 39.0 11.0 39.0 11 32.73175784920959 38.0 33.0 39.0 10.0 39.0 12 32.622304751020145 37.0 32.0 39.0 10.0 39.0 13 32.55979350691206 37.0 32.0 39.0 10.0 39.0 14 33.418104784060894 38.0 32.0 41.0 10.0 41.0 15 33.470503391706615 38.0 33.0 41.0 10.0 41.0 16 33.42592584711418 38.0 32.0 41.0 10.0 41.0 17 33.266323156343496 38.0 32.0 41.0 10.0 41.0 18 33.237386964069536 38.0 32.0 41.0 10.0 41.0 19 33.379262556742844 39.0 32.0 41.0 10.0 41.0 20 33.36939327663272 39.0 32.0 41.0 8.0 41.0 21 33.32128648459175 39.0 32.0 41.0 8.0 41.0 22 33.242895948329526 39.0 32.0 41.0 8.0 41.0 23 33.203619239002634 38.0 32.0 40.0 8.0 41.0 24 33.21668929351987 38.0 32.0 41.0 8.0 41.0 25 33.121250658203486 38.0 32.0 40.0 2.0 41.0 26 33.065062423419164 38.0 32.0 40.0 2.0 41.0 27 33.01339736687653 38.0 32.0 40.0 2.0 41.0 28 32.896657119411365 38.0 32.0 40.0 2.0 41.0 29 32.88006362085622 38.0 32.0 40.0 2.0 41.0 30 32.71043263652427 38.0 32.0 40.0 2.0 41.0 31 32.702015756646745 38.0 32.0 40.0 2.0 41.0 32 32.563511945546985 38.0 31.0 40.0 2.0 41.0 33 32.51927448081722 38.0 31.0 40.0 2.0 41.0 34 32.432889939865056 38.0 31.0 40.0 2.0 41.0 35 32.359701580887744 38.0 31.0 40.0 2.0 41.0 36 32.26494194461457 38.0 31.0 40.0 2.0 41.0 37 32.15960307766472 38.0 31.0 40.0 2.0 41.0 38 32.06955194187923 38.0 31.0 40.0 2.0 41.0 39 31.951863030223777 38.0 30.0 40.0 2.0 41.0 40 31.87918653206365 38.0 30.0 40.0 2.0 41.0 41 31.762739550282102 38.0 30.0 40.0 2.0 41.0 42 31.612664899076755 38.0 30.0 40.0 2.0 41.0 43 31.50158336386572 38.0 30.0 40.0 2.0 41.0 44 31.34126762737616 38.0 30.0 40.0 2.0 41.0 45 31.18769119819924 37.0 30.0 40.0 2.0 41.0 46 31.03108848382802 37.0 30.0 40.0 2.0 41.0 47 30.85448063924181 37.0 30.0 40.0 2.0 41.0 48 30.675279056999937 37.0 30.0 40.0 2.0 41.0 49 30.488516404500817 37.0 29.0 40.0 2.0 41.0 50 30.265491721047848 37.0 29.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 2.072095402001594 1101 2 2.1754389947173394 1101 3 2.181308799106894 1101 4 2.544324993700947 1101 5 2.6084798432695955 1101 6 2.490295786788323 1101 7 2.744091501198003 1101 8 2.8599278750163712 1101 9 3.069930974750619 1101 10 3.1230440639715127 1101 11 3.2391133834191805 1101 12 3.1188619742598362 1101 13 3.105318102685416 1101 14 3.668444676379835 1101 15 3.6761490159894663 1101 16 3.427796363758489 1101 17 3.607017674940316 1101 18 3.5959761616934074 1101 19 3.476036792121505 1101 20 3.508910301090708 1101 21 3.363530774358864 1101 22 3.556426418381477 1101 23 3.5324261442635887 1101 24 3.6002086575436465 1101 25 3.4592817900675996 1101 26 3.492216034357341 1101 27 3.453499931108645 1101 28 3.5931048306134343 1101 29 3.5492061241252344 1101 30 3.7356456155127518 1101 31 3.8320374479223744 1101 32 3.9131381087038903 1101 33 3.881201781711553 1101 34 3.980939739772495 1101 35 3.8478930714007973 1101 36 3.830677273911533 1101 37 3.8513861322339125 1101 38 3.983238912025165 1101 39 3.8980719491800997 1101 40 3.8593699323990123 1101 41 3.878909268828778 1101 42 4.069691510779229 1101 43 3.941686698090262 1101 44 4.104854283917923 1101 45 4.06138060252654 1101 46 3.869142873101797 1101 47 4.04770176708044 1101 48 4.113568134234612 1101 49 4.067779903246269 1101 50 4.307144987921024 1102 1 1.116216384164236 1102 2 1.1077050189698383 1102 3 1.1380200431688898 1102 4 1.3146068809215166 1102 5 1.323583182958064 1102 6 1.347003320839221 1102 7 1.3635381956286459 1102 8 1.3849918871436309 1102 9 1.618322006455621 1102 10 1.6577548267425755 1102 11 1.5158904606897607 1102 12 1.6355840913971562 1102 13 1.5085877917648318 1102 14 1.787050988335757 1102 15 1.664824098847582 1102 16 1.7615056545068626 1102 17 1.8877708158544806 1102 18 1.8244211776688246 1102 19 1.8078196199092815 1102 20 1.7272588899800496 1102 21 1.6118387855431777 1102 22 1.6990084966026586 1102 23 1.6537677593425713 1102 24 1.604895686348783 1102 25 1.7483052242011183 1102 26 1.7978441500629145 1102 27 1.6632492339658 1102 28 1.7962136466824887 1102 29 1.561432126452587 1102 30 1.848071944003486 1102 31 1.839159370002612 1102 32 1.879521799206394 1102 33 1.8088207644380603 1102 34 1.7983786108435424 1102 35 1.805886362488124 1102 36 1.8297672385047505 1102 37 1.8881236148802287 1102 38 1.8839338967109 1102 39 1.9506400636124326 1102 40 1.9873588191113 1102 41 1.913369641940406 1102 42 1.7713553713590358 1102 43 1.470543294323985 1102 44 1.4085064217288092 1102 45 1.5413398299376624 1102 46 1.4111604810743081 1102 47 1.4293236135890552 1102 48 1.396557098178647 1102 49 1.2126961125734717 1102 50 1.1081118427973742 1103 1 0.12673024464961458 1103 2 0.17839925350628505 1103 3 0.17087844985241674 1103 4 0.3223196634847696 1103 5 0.34504319323892574 1103 6 0.34190601333519055 1103 7 0.2530666446130887 1103 8 0.39711737064478925 1103 9 0.4080708125103243 1103 10 0.410769447743526 1103 11 0.42894792145443006 1103 12 0.24896876630276665 1103 13 0.30095684837485237 1103 14 0.24503774182237237 1103 15 0.22778288503337052 1103 16 0.16814149855370175 1103 17 0.4292990287726113 1103 18 0.2502893699853246 1103 19 0.2607269561831842 1103 20 0.2891813222385693 1103 21 0.09154299844306024 1103 22 0.014622957546080784 1103 23 0.25111810028448645 1103 24 0.19915656642567114 1103 25 0.18673161698524154 1103 26 0.25998494366198344 1103 27 0.2036889188799904 1103 28 0.3074221636585932 1103 29 0.32726218322539324 1103 30 0.4773548868722983 1103 31 0.3077170048234166 1103 32 0.6059753960672793 1103 33 0.4317330529726817 1103 34 0.42738250793345856 1103 35 0.3409546834436554 1103 36 0.4898621810690713 1103 37 0.4560745763800327 1103 38 0.410271114019352 1103 39 0.444561926232673 1103 40 0.4587115446651069 1103 41 0.4219899825886628 1103 42 0.2529943016593137 1103 43 0.2198437197850467 1103 44 0.30158484620854153 1103 45 0.10342733755350864 1103 46 0.22618313688306202 1103 47 0.251199106284826 1103 48 0.31114000920132057 1103 49 0.31473948320128997 1103 50 0.34946619914745725 1104 1 0.20549826278640282 1104 2 0.20816905653190076 1104 3 0.2884461836992216 1104 4 0.14931918317531867 1104 5 0.2949360143942066 1104 6 0.17810421913472396 1104 7 0.21578458324464833 1104 8 0.32922674627576143 1104 9 0.2955001260193768 1104 10 0.0893714004318582 1104 11 0.10282065512782879 1104 12 0.21197543695063104 1104 13 0.21767221110349766 1104 14 0.3155147209372231 1104 15 0.16501544269782187 1104 16 0.32423838803929783 1104 17 0.3095526806209463 1104 18 0.17550314569744074 1104 19 0.30035889946960737 1104 20 0.20947073837515973 1104 21 0.28173995857357426 1104 22 0.23278856026318095 1104 23 0.2851425138770054 1104 24 0.2953205994667769 1104 25 0.3466020949620301 1104 26 0.09215539128093297 1104 27 0.3182953383036846 1104 28 0.1261016642317827 1104 29 0.22668129859135178 1104 30 0.2520728891650563 1104 31 0.222756747936252 1104 32 0.35388253433986705 1104 33 0.18168281288921406 1104 34 0.12503115325652203 1104 35 0.36316353206446195 1104 36 0.18012945356225885 1104 37 0.25652987268250627 1104 38 0.17228847072109232 1104 39 0.2835746456440589 1104 40 0.34355462219921407 1104 41 0.24548896804778053 1104 42 0.2955581041898938 1104 43 0.18357631806913943 1104 44 0.3080544149704707 1104 45 0.36542913526959353 1104 46 0.2783047886724894 1104 47 0.22573213480456644 1104 48 0.17140986614891673 1104 49 0.32966850809709314 1104 50 0.3402867539215002 1105 1 0.2557407723214844 1105 2 0.2673822007560247 1105 3 0.15285384939474866 1105 4 0.2546604119363707 1105 5 0.20233531391761161 1105 6 0.2390921849459744 1105 7 0.23566396715874305 1105 8 0.2709287113419947 1105 9 0.2583411156927937 1105 10 0.09519154010361319 1105 11 0.17233362749865933 1105 12 0.23824620720068168 1105 13 0.3386905661226578 1105 14 0.2990250311314071 1105 15 0.36166629874298906 1105 16 0.21803956477398856 1105 17 0.3104508475712109 1105 18 0.03854216011693268 1105 19 0.1716968739638105 1105 20 0.30130323748401366 1105 21 0.35748425675162565 1105 22 0.3759823206668358 1105 23 0.2494324080163608 1105 24 0.29142285808134716 1105 25 0.33249703952379406 1105 26 0.3301935975953896 1105 27 0.36954324196776156 1105 28 0.36321550984649065 1105 29 0.33436792682714156 1105 30 0.4372591311823655 1105 31 0.1542940891881983 1105 32 0.3460656507183586 1105 33 0.387373521999713 1105 34 0.3537505152079916 1105 35 0.2843963637336202 1105 36 0.31048047432710746 1105 37 0.21503351183028485 1105 38 0.3207741487591136 1105 39 0.3450116949304416 1105 40 0.32308225638198707 1105 41 0.3276127260460697 1105 42 0.45511716195502316 1105 43 0.5011644957042272 1105 44 0.43549882124511186 1105 45 0.5138364549847019 1105 46 0.46874204148987886 1105 47 0.20566660807501336 1105 48 0.39276555207627695 1105 49 0.45051052902400457 1105 50 0.5692589134211481 1106 1 -0.08843346229040705 1106 2 -0.16202787474979274 1106 3 -0.17931478849871496 1106 4 -0.19736355356686985 1106 5 -0.23773624973788543 1106 6 -0.3285449827736997 1106 7 -0.29797593932423183 1106 8 -0.23547222082638086 1106 9 -0.22351777795984162 1106 10 -0.176473340157159 1106 11 -0.32531597590157446 1106 12 -0.34894305529326886 1106 13 -0.302062289183759 1106 14 -0.22079204473109826 1106 15 -0.26759171889386835 1106 16 -0.3269501626563809 1106 17 -0.15938622603106722 1106 18 -0.48994005116514927 1106 19 -0.35007792764801593 1106 20 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-0.4571158502853443 1107 6 -0.29692622613882236 1107 7 -0.27534782586672435 1107 8 -0.22142322362509148 1107 9 -0.220262246336155 1107 10 -0.38490709227374253 1107 11 -0.2125075363370854 1107 12 -0.448790836941086 1107 13 -0.29080860435909983 1107 14 -0.4644265649581527 1107 15 -0.3630364640706958 1107 16 -0.4262008601410514 1107 17 -0.428998650470227 1107 18 -0.27173230162794226 1107 19 -0.4180130440270631 1107 20 -0.3334399571505102 1107 21 -0.2692048555953406 1107 22 -0.6212156231583066 1107 23 -0.5416147142102901 1107 24 -0.5913843829250851 1107 25 -0.4957401780303101 1107 26 -0.19873124043343182 1107 27 -0.32532848825876926 1107 28 -0.357277016268128 1107 29 -0.36941710983663256 1107 30 -0.4351174454366742 1107 31 -0.4326558651293766 1107 32 -0.41976329478226404 1107 33 -0.4015992291343977 1107 34 -0.25340323697130884 1107 35 -0.23948170393556012 1107 36 -0.37559633462281283 1107 37 -0.2807465504644284 1107 38 -0.40414680448258267 1107 39 -0.27088315712373046 1107 40 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0.03455424867168233 2215 16 0.004343580463938679 2215 17 0.3042394198074234 2215 18 0.08645600872706893 2215 19 0.03160298242401183 2215 20 -0.009502554771493976 2215 21 0.09416471887593758 2215 22 0.05371032817851784 2215 23 -0.012831471605004197 2215 24 -0.0901005018013592 2215 25 -0.060314785640557034 2215 26 -0.05968128909437809 2215 27 -0.04913579424515291 2215 28 0.028317952150949566 2215 29 -0.10252597403057706 2215 30 0.03165811714119826 2215 31 -0.022668101312760314 2215 32 -0.05775700586254828 2215 33 0.04754843917432794 2215 34 -0.11944006571314958 2215 35 -0.02477263185447498 2215 36 -0.1075796813942489 2215 37 -0.04729252462654898 2215 38 0.0474941739891932 2215 39 -0.07860931367676827 2215 40 -0.0870853251660968 2215 41 -0.19970145133453343 2215 42 0.03011369573149736 2215 43 -0.06338800979861503 2215 44 -0.13695992173536808 2215 45 -0.2366992697404804 2215 46 -0.1831424977644609 2215 47 -0.08035025423449227 2215 48 -0.22135170998567943 2215 49 -0.2758876245931994 2215 50 -0.27654285711911086 2216 1 0.2396231375425053 2216 2 0.2306339882410171 2216 3 0.2579930644670334 2216 4 0.383047891712998 2216 5 0.4440672172629512 2216 6 0.20126287019204625 2216 7 0.43332940737585446 2216 8 0.394890143417566 2216 9 0.46212236406187657 2216 10 0.39972340169747866 2216 11 0.28096744097394577 2216 12 0.3208856832196787 2216 13 0.41965554707354613 2216 14 0.4161513554359857 2216 15 0.13661671886720228 2216 16 0.2767350921472982 2216 17 0.3512434732170533 2216 18 0.38729887949483555 2216 19 0.3156969261531515 2216 20 0.20539253344977482 2216 21 -0.027898943498883 2216 22 0.24993827286139947 2216 23 0.19723171389809835 2216 24 0.20145886872796837 2216 25 -0.07653915378553222 2216 26 0.24341837709541636 2216 27 0.19443716712824255 2216 28 0.2255127542491806 2216 29 0.033390811854019375 2216 30 0.243328360345771 2216 31 -0.08554125343484031 2216 32 0.05552744811933508 2216 33 0.12160542534505225 2216 34 0.09453466008560696 2216 35 0.13032155031051929 2216 36 -0.036987168280276705 2216 37 -0.2366356338301756 2216 38 -0.21390306015482352 2216 39 -0.13700464033389537 2216 40 -0.1684706375170748 2216 41 -0.1522467541480772 2216 42 -0.01436055319554086 2216 43 -0.2285596836183572 2216 44 -0.44799473337103635 2216 45 -0.4426887785626086 2216 46 -0.6478659121659867 2216 47 -0.38740199481627613 2216 48 -0.46898761842630776 2216 49 -0.27659935563755056 2216 50 -0.4894326997514433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 175206.0 3 8397.0 4 15140.0 5 11696.0 6 9468.0 7 8144.0 8 7134.0 9 6462.0 10 6378.0 11 6208.0 12 6486.0 13 6788.0 14 7284.0 15 7651.0 16 7995.0 17 8759.0 18 9539.0 19 10830.0 20 13945.0 21 18248.0 22 22301.0 23 24531.0 24 26097.0 25 27010.0 26 29966.0 27 33630.0 28 38395.0 29 43813.0 30 51066.0 31 61563.0 32 74194.0 33 91397.0 34 115940.0 35 148604.0 36 192880.0 37 261153.0 38 401043.0 39 589346.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.36604858963666 16.956111961699513 12.614084040199403 25.063755408464427 2 16.42065070276906 18.931876225900705 38.837812247679864 25.809660823650372 3 20.087812823987456 23.44289474815488 27.49628884896419 28.973003578893476 4 13.288637546629653 15.442315557263072 35.45329134082713 35.81575555528015 5 14.990336048379008 36.69803820426701 33.24004357394656 15.071582173407421 6 34.20098195009575 35.280600582140096 16.422288865825575 14.096128601938581 7 30.192951909506228 30.8953764470619 20.072131482601193 18.83954016083068 8 27.261989882922332 34.72065383559729 18.70861894960314 19.308737331877236 9 26.73163759569882 16.32640420207626 18.077591920418225 38.864366281806696 10 15.344360166532741 28.847431175189158 32.12854110444835 23.67966755382974 11 36.43483797797128 22.735339827246175 21.844900897832158 18.98492129695039 12 24.32524084301054 24.2470825149097 28.798963153709828 22.628713488369932 13 28.971271279834543 21.1810683164994 24.6521892115463 25.19547119211976 14 22.769656097170106 20.59940867386715 25.64027427994547 30.990660949017286 15 24.478006196571602 28.50957124121881 22.39003098285801 24.622391579351575 16 24.60836307611313 27.53389128465582 24.040979227797436 23.816766411433616 17 23.722185533264327 27.4787692226698 25.29917415005276 23.499871094013113 18 23.635402198469215 26.90809878401333 25.987785938034698 23.46871307948276 19 24.524441398907864 26.67181568825578 25.7817318134041 23.02201109943225 20 24.390759490926435 25.945917902750722 25.986376490192377 23.67694611613047 21 24.73289063558748 25.82835459702244 25.91779761098097 23.520957156409114 22 24.534118079823433 25.303311617281203 25.95928482231881 24.203285480576557 23 24.35245736955368 25.58705537999736 25.937049128035156 24.123438122413805 24 23.999558603172634 25.779897859225386 25.877198756345077 24.3433447812569 25 24.334914215705254 25.561001562812198 25.972459500860403 24.131624720622153 26 24.052367503370643 26.0631752416369 26.132330517301593 23.752126737690865 27 24.13572541452037 25.802595998674278 25.949842493100363 24.111836093704987 28 24.078892591524042 26.056203032632457 25.777414614027716 24.08748976181578 29 24.226751430565034 25.929760786448718 25.794429218298532 24.049058564687716 30 24.13963339554406 26.026941632915413 25.938504309577098 23.894920661963432 31 24.273792984111882 25.514516398135527 25.74751737248916 24.464173245263428 32 23.944185369543348 26.03195014819979 25.728892513829983 24.294971968426886 33 24.16057032209913 25.80532524633774 25.795763617965335 24.238340813597798 34 24.560894179126144 25.67923743165433 25.755378139062273 24.004490250157257 35 24.40389297229542 25.81051006515514 25.770172796034714 24.015424166514723 36 24.29702458236459 25.90903495386016 25.648160406684024 24.145780057091226 37 24.25485097406607 25.50948631917499 26.03113806021429 24.20452464654465 38 24.410344903668282 25.801254694847948 25.840505036546556 23.947895364937214 39 24.50589152046093 25.821307643086005 25.664221076310085 24.008579760142982 40 24.407382503310764 25.683104531784274 26.017142018772528 23.892370946132438 41 24.046356118911703 25.852930293191882 25.91656332105572 24.18415026684069 42 24.51925663449648 25.76032790755732 25.837311870199386 23.883103587746817 43 24.18905612821521 25.597022204194364 25.75880844818351 24.45511321940692 44 24.397442774770507 25.8269220795607 25.615561639763783 24.16007350590501 45 24.29491069410603 25.845921403382754 25.510153533689795 24.349014368821422 46 24.264341082271354 25.830472792287956 25.539103950836108 24.366082174604585 47 24.257211734760027 25.77721325185893 25.54442371333952 24.421151300041526 48 24.100702162834907 25.975767951901226 25.73378028619018 24.18974959907369 49 24.443937051449005 25.68201035984698 25.523536047584862 24.35051654111915 50 24.455689697074774 25.7479477585948 25.69330388033455 24.103058663995878 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1881.0 1 1447.5 2 1014.0 3 944.5 4 875.0 5 831.0 6 787.0 7 773.5 8 760.0 9 838.0 10 916.0 11 1193.5 12 1471.0 13 2441.0 14 3411.0 15 4737.0 16 6063.0 17 6767.5 18 7472.0 19 7955.0 20 8438.0 21 9684.5 22 10931.0 23 13806.5 24 16682.0 25 20661.5 26 24641.0 27 29709.0 28 34777.0 29 40202.0 30 45627.0 31 51925.0 32 58223.0 33 65313.0 34 72403.0 35 81208.5 36 90014.0 37 96256.5 38 102499.0 39 109861.0 40 117223.0 41 126121.5 42 135020.0 43 140793.0 44 146566.0 45 154714.5 46 162863.0 47 173226.5 48 183590.0 49 185396.5 50 187203.0 51 189769.5 52 192336.0 53 188319.5 54 184303.0 55 179874.5 56 175446.0 57 165829.0 58 156212.0 59 142811.0 60 129410.0 61 115669.0 62 101928.0 63 89068.5 64 76209.0 65 64889.5 66 53570.0 67 45241.0 68 36912.0 69 29746.5 70 22581.0 71 18331.0 72 14081.0 73 11523.0 74 8965.0 75 6840.0 76 4715.0 77 3711.0 78 2707.0 79 2206.5 80 1706.0 81 1302.5 82 899.0 83 746.0 84 593.0 85 454.5 86 316.0 87 242.5 88 169.0 89 140.5 90 112.0 91 89.5 92 67.0 93 49.0 94 31.0 95 26.5 96 22.0 97 17.5 98 13.0 99 23.5 100 34.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08360780241476047 2 0.09293194882010858 3 0.10074720846276551 4 0.10210133760877042 5 0.09324146405348115 6 0.05648653009049065 7 0.05219200622744649 8 0.059194788382500474 9 0.061245326803593625 10 0.04530529228490723 11 0.048052239981088624 12 0.05683473472803477 13 0.050837877081441586 14 0.05188249099407395 15 0.051186081718985706 16 0.05520977975282887 17 0.05451337047774063 18 0.05551929498620143 19 0.06468868377486327 20 0.06960223810465252 21 0.07881031629748593 22 0.0817120216103536 23 0.057956727449010266 24 0.07648895204719179 25 0.08368518122310362 26 0.08097692293109379 27 0.07517351230535844 28 0.09447952498697135 29 0.08283401433132909 30 0.07997099842263299 31 0.055557984390372996 32 0.05223069563161806 33 0.055906189027917115 34 0.05076049827309844 35 0.056796045323863203 36 0.05621570426128967 37 0.05981381884924558 38 0.04913554329789255 39 0.05408778703185338 40 0.05586749962374555 41 0.04344820088467192 42 0.039927465105059144 43 0.03988877570088757 44 0.036716244558818924 45 0.02936525776622082 46 0.026192726624152167 47 0.0312610385706277 48 0.02596059019912276 49 0.025999279603294324 50 0.026424863049181586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2584687.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.150167768315 #Duplication Level Percentage of deduplicated Percentage of total 1 77.7469360470058 45.98744310652851 2 11.918150539251961 14.099212077695746 3 4.388910143195158 7.78814313870159 4 2.131233077544568 5.04251176360574 5 1.1229686786110737 3.3211892869204007 6 0.6425386304397172 2.280376067287957 7 0.4416099053503485 1.8284909992735976 8 0.30360367200191446 1.4366566507191776 9 0.2050525024177894 1.0916000927392553 >10 1.0018294162447137 10.40216932756729 >50 0.06194197717825275 2.5387960218047416 >100 0.03357790004205467 3.390787793425565 >500 0.0013180085731561712 0.5249465434329171 >1k 3.29502143284489E-4 0.2676771302974475 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.8689404171568936E-5 3 0.0 0.0 0.0 0.0 3.8689404171568936E-5 4 0.0 0.0 0.0 0.0 3.8689404171568936E-5 5 0.0 0.0 0.0 0.0 7.737880834313787E-5 6 0.0 0.0 0.0 0.0 7.737880834313787E-5 7 0.0 0.0 0.0 0.0 1.1606821251470681E-4 8 0.0 0.0 0.0 0.0 1.5475761668627574E-4 9 0.0 0.0 0.0 0.0 2.3213642502941363E-4 10 0.0 0.0 0.0 0.0 2.7082582920098255E-4 11 0.0 0.0 0.0 0.0 2.7082582920098255E-4 12 0.0 0.0 0.0 0.0 3.868940417156894E-4 13 0.0 0.0 0.0 0.0 3.868940417156894E-4 14 0.0 0.0 0.0 0.0 4.6427285005882726E-4 15 0.0 0.0 0.0 0.0 5.029622542303962E-4 16 0.0 0.0 0.0 3.8689404171568936E-5 5.029622542303962E-4 17 0.0 0.0 0.0 3.8689404171568936E-5 5.029622542303962E-4 18 0.0 0.0 0.0 3.8689404171568936E-5 5.029622542303962E-4 19 0.0 0.0 0.0 3.8689404171568936E-5 5.029622542303962E-4 20 0.0 0.0 0.0 3.8689404171568936E-5 5.416516584019651E-4 21 0.0 0.0 0.0 3.8689404171568936E-5 5.803410625735341E-4 22 0.0 0.0 0.0 3.8689404171568936E-5 5.803410625735341E-4 23 0.0 0.0 0.0 1.1606821251470681E-4 7.350986792598098E-4 24 0.0 0.0 0.0 2.3213642502941363E-4 7.737880834313787E-4 25 0.0 0.0 0.0 2.3213642502941363E-4 7.737880834313787E-4 26 0.0 0.0 0.0 3.4820463754412043E-4 7.737880834313787E-4 27 0.0 0.0 0.0 5.029622542303962E-4 7.737880834313787E-4 28 0.0 0.0 0.0 0.001315439741833344 7.737880834313787E-4 29 0.0 0.0 0.0 0.0022439854419509984 7.737880834313787E-4 30 0.0 0.0 0.0 0.003830251012985325 7.737880834313787E-4 31 0.0 0.0 0.0 0.008511668917745166 7.737880834313787E-4 32 0.0 0.0 0.0 0.014276390139308938 8.124774876029476E-4 33 0.0 0.0 0.0 0.020582763019274673 8.124774876029476E-4 34 3.8689404171568936E-5 0.0 0.0 0.03052593989136789 8.124774876029476E-4 35 3.8689404171568936E-5 0.0 0.0 0.040894700209348364 8.124774876029476E-4 36 3.8689404171568936E-5 0.0 0.0 0.0562930830696328 8.124774876029476E-4 37 3.8689404171568936E-5 0.0 0.0 0.07289083745923587 8.124774876029476E-4 38 3.8689404171568936E-5 0.0 0.0 0.09362835809519683 8.124774876029476E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4420 0.0 18.821867 1 CGTGCCG 285 0.0 18.523186 10 TACACCG 240 0.0 17.420132 5 GCGCACT 360 0.0 17.104246 42 GTGCCGA 335 0.0 17.072403 11 CGCACGA 210 9.094947E-12 16.757774 35 TGCGATA 295 0.0 16.40528 13 CGACAGG 430 0.0 16.36806 34 TTGCGCG 190 6.166374E-10 16.212496 18 GCACCGT 500 0.0 15.838857 6 GCGATAC 310 0.0 15.612683 14 CGCACTC 430 0.0 15.341789 43 TACCGAT 275 0.0 15.198903 7 AGCACCG 470 0.0 14.981704 5 GTACATG 6765 0.0 14.835092 1 TATCAAC 5490 0.0 14.831919 2 CAACGCA 5435 0.0 14.65609 5 TATACTG 500 0.0 14.522889 5 ATCAACG 5525 0.0 14.498966 3 TCAACGC 5510 0.0 14.418057 4 >>END_MODULE