FastQCFastQC Report
Mon 6 Feb 2017
SRR2077096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584687
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27580.10670537670518714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCG2000.020.9044210
GGTATCA30750.020.0348191
GTATCAA54650.019.4057041
TACCGTA1105.6187633E-516.0021487
CGCACGA2500.015.83660835
GTACATG69200.014.88032151
GTATAGG3700.014.8665721
GTATGCG900.00435466414.6697711
TATCAAC73350.014.3689412
ATCAACG74200.014.2932993
ATACCGT1554.566664E-614.1949036
TACATGG70850.014.1616672
TCAACGC75050.014.0434614
CAACGCA75150.013.99603755
ACCGTGC5600.013.7518448
GCACCGT3550.013.6351056
AACGCAG78600.013.4363986
CGTCGTA1150.00142236213.39413810
ACATGGG74850.013.1990863
TACCGCG1353.2053067E-413.0390417
Produced by FastQC (version 0.11.5)