FastQCFastQC Report
Mon 6 Feb 2017
SRR2077095_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077095_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511254
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102750.6798989448497738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59050.3907351113710865No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT35240.23318383276404892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.21750149213831693No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT30440.20142213023092082No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT27790.18388702362408968No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA25360.16780766171669356No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA25310.16747681064864015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24670.16324191697755638No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG23200.1535148955767859No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG23020.1523238317317936No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC23000.1521914913045722No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA21600.14292766139907653No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT21440.1418689379813056No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA20530.1358474485427334No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT19810.13108319316276418No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC19520.12916425696805434No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA19370.12817170376389408No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA18490.12234872496615394No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC18190.12036361855783342No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC17670.11692276745007789No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG16830.11136446950678046No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG16740.11076893758428433No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC16710.11057042694345226No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC16330.10805595882624629No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC15760.10428425665043731No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT15380.10176978853323135No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT15270.10104191618351382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG300.005739545429.3395182
AGTTTAC1350.026.0726614
GTATGGT1800.025.674631
CCCTATA4150.023.8604492
GCCTATC1550.022.7061927
TAGTCGG4100.022.53387340
CATTCCG2750.022.3958199
TATCTGC3650.022.30754926
CAATTTA4250.022.26056715
TACTCGC1004.6335117E-821.9966227
CGTACGC500.00258402821.99516510
TTAGTCG4150.021.73448239
TCTGCAT3750.021.7126828
CTGAGTT4200.021.47289335
AGTCGGC4200.021.47004941
CTTTAGG3100.021.2948112
ACTACCT1551.8189894E-1221.28705434
CCTATAC4550.021.2799153
TAGGCAT3000.021.2697415
CGTACTT1451.2732926E-1121.2409339
Produced by FastQC (version 0.11.5)